Uncovered Microbial Diversity in Antarctic Cryptoendolithic Communities Sampling Three Representative Locations of the Victoria Land
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Metagenomics DNA Extraction and Amplicon Sequencing
2.3. Raw Data Processing
2.4. Accumulation and Extrapolation
2.5. Downstream Analysis
3. Results
3.1. Estimating and Determining Sampling Effort Across Sampling Areas
3.2. Differences in Community’ Composition by Site
3.3. Differences in Alpha Diversity and Taxonomic Composition
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Site | Samples | N° of Species (Computed) | |
---|---|---|---|
Fungi | |||
Trio Nunatak | 23 | 150 | |
Mt New Zealand | 28 | 190 | |
Bacteria | |||
Battleship Promontory | 12 | 335 | |
Trio Nunatak | 32 | 350 | |
Mt New Zealand | 34 | 465 |
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Coleine, C.; Pombubpa, N.; Zucconi, L.; Onofri, S.; Turchetti, B.; Buzzini, P.; Stajich, J.E.; Selbmann, L. Uncovered Microbial Diversity in Antarctic Cryptoendolithic Communities Sampling Three Representative Locations of the Victoria Land. Microorganisms 2020, 8, 942. https://doi.org/10.3390/microorganisms8060942
Coleine C, Pombubpa N, Zucconi L, Onofri S, Turchetti B, Buzzini P, Stajich JE, Selbmann L. Uncovered Microbial Diversity in Antarctic Cryptoendolithic Communities Sampling Three Representative Locations of the Victoria Land. Microorganisms. 2020; 8(6):942. https://doi.org/10.3390/microorganisms8060942
Chicago/Turabian StyleColeine, Claudia, Nuttapon Pombubpa, Laura Zucconi, Silvano Onofri, Benedetta Turchetti, Pietro Buzzini, Jason E. Stajich, and Laura Selbmann. 2020. "Uncovered Microbial Diversity in Antarctic Cryptoendolithic Communities Sampling Three Representative Locations of the Victoria Land" Microorganisms 8, no. 6: 942. https://doi.org/10.3390/microorganisms8060942