Expression of Bacillus cereus Virulence-Related Genes in an Ocular Infection-Related Environment
Abstract
1. Introduction
2. Materials and Methods
2.1. Bacterial Strain, Growth Environments, and Growth Curves
2.2. RNA Preparation and Quantitative PCR Analysis
2.3. RNA Sequencing
2.4. Statistics
2.5. Data Availability
3. Results
3.1. Growth of B. cereus in BHI, LB, and Rabbit Vitreous
3.2. rRNA Depletion and RNA-Seq Analysis of Depleted RNA Samples
3.3. Virulence Factor Expression in Ex Vivo Vitreous
3.4. Virulence-Related Transcriptional Regulatory Gene Expression in Ex Vivo Vitreous
3.5. Expression of Genes Associated with Motility and Chemotaxis in Ex Vivo Vitreous
3.6. Expression of Superoxide Dismutase in Ex Vivo Vitreous
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Media | RiboZero Treated Sample (Mean Threshold Cycle (CT)) | Untreated Sample (Mean Threshold Cycle (CT)) | rRNA Fold Depletion |
---|---|---|---|
LB | 19.536 | 7.225 | 5,078 |
BHI | 17.296 | 4.1825 | 8,856 |
Vitreous | 25.2 | 5.8 | 691,802 |
Environment | Mean Number of Reads | Percentage of Reads Mapped |
---|---|---|
BHI | 9,609,820 | 84% |
LB | 8,565,599 | 87% |
Vitreous | 8,350,233 | 94% |
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Coburn, P.S.; Miller, F.C.; Enty, M.A.; Land, C.; LaGrow, A.L.; Mursalin, M.H.; Callegan, M.C. Expression of Bacillus cereus Virulence-Related Genes in an Ocular Infection-Related Environment. Microorganisms 2020, 8, 607. https://doi.org/10.3390/microorganisms8040607
Coburn PS, Miller FC, Enty MA, Land C, LaGrow AL, Mursalin MH, Callegan MC. Expression of Bacillus cereus Virulence-Related Genes in an Ocular Infection-Related Environment. Microorganisms. 2020; 8(4):607. https://doi.org/10.3390/microorganisms8040607
Chicago/Turabian StyleCoburn, Phillip S., Frederick C. Miller, Morgan A. Enty, Craig Land, Austin L. LaGrow, Md Huzzatul Mursalin, and Michelle C. Callegan. 2020. "Expression of Bacillus cereus Virulence-Related Genes in an Ocular Infection-Related Environment" Microorganisms 8, no. 4: 607. https://doi.org/10.3390/microorganisms8040607
APA StyleCoburn, P. S., Miller, F. C., Enty, M. A., Land, C., LaGrow, A. L., Mursalin, M. H., & Callegan, M. C. (2020). Expression of Bacillus cereus Virulence-Related Genes in an Ocular Infection-Related Environment. Microorganisms, 8(4), 607. https://doi.org/10.3390/microorganisms8040607