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Article

Isoprene Oxidation by the Gram-Negative Model bacterium Variovorax sp. WS11

1
School of Environmental Sciences, Norwich Research Park, University of East Anglia, Norwich NR4 7TJ, UK
2
School of Biological Sciences, Norwich Research Park, University of East Anglia, Norwich NR4 7TJ, UK
3
School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore 54000, Pakistan
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(3), 349; https://doi.org/10.3390/microorganisms8030349
Received: 11 February 2020 / Revised: 27 February 2020 / Accepted: 28 February 2020 / Published: 29 February 2020
(This article belongs to the Special Issue Microbial Cycling of Atmospheric Trace Gases)
Plant-produced isoprene (2-methyl-1,3-butadiene) represents a significant portion of global volatile organic compound production, equaled only by methane. A metabolic pathway for the degradation of isoprene was first described for the Gram-positive bacterium Rhodococcus sp. AD45, and an alternative model organism has yet to be characterised. Here, we report the characterisation of a novel Gram-negative isoprene-degrading bacterium, Variovorax sp. WS11. Isoprene metabolism in this bacterium involves a plasmid-encoded iso metabolic gene cluster which differs from that found in Rhodococcus sp. AD45 in terms of organisation and regulation. Expression of iso metabolic genes is significantly upregulated by both isoprene and epoxyisoprene. The enzyme responsible for the initial oxidation of isoprene, isoprene monooxygenase, oxidises a wide range of alkene substrates in a manner which is strongly influenced by the presence of alkyl side-chains and differs from other well-characterised soluble diiron monooxygenases according to its response to alkyne inhibitors. This study presents Variovorax sp. WS11 as both a comparative and contrasting model organism for the study of isoprene metabolism in bacteria, aiding our understanding of the conservation of this biochemical pathway across diverse ecological niches. View Full-Text
Keywords: isoprene; microbiology; monooxygenase; mutagenesis; isoprene-fed fermentor isoprene; microbiology; monooxygenase; mutagenesis; isoprene-fed fermentor
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MDPI and ACS Style

Dawson, R.A.; Larke-Mejía, N.L.; Crombie, A.T.; Ul Haque, M.F.; Murrell, J.C. Isoprene Oxidation by the Gram-Negative Model bacterium Variovorax sp. WS11. Microorganisms 2020, 8, 349. https://doi.org/10.3390/microorganisms8030349

AMA Style

Dawson RA, Larke-Mejía NL, Crombie AT, Ul Haque MF, Murrell JC. Isoprene Oxidation by the Gram-Negative Model bacterium Variovorax sp. WS11. Microorganisms. 2020; 8(3):349. https://doi.org/10.3390/microorganisms8030349

Chicago/Turabian Style

Dawson, Robin A., Nasmille L. Larke-Mejía, Andrew T. Crombie, Muhammad F. Ul Haque, and J. C. Murrell 2020. "Isoprene Oxidation by the Gram-Negative Model bacterium Variovorax sp. WS11" Microorganisms 8, no. 3: 349. https://doi.org/10.3390/microorganisms8030349

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