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Open AccessArticle

Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models

1
Department of Biology, University of Naples Federico II, via Cinthia, 80100 Naples, Italy
2
Department of Sciences and Technologies, University of Naples Parthenope, Business District, Block C4, 80143 Naples, Italy
3
Department of Chemical Sciences, University of Naples Federico II, via Cinthia; 80100 Naples, Italy
*
Authors to whom correspondence should be addressed.
Microorganisms 2020, 8(3), 336; https://doi.org/10.3390/microorganisms8030336
Received: 30 January 2020 / Revised: 24 February 2020 / Accepted: 26 February 2020 / Published: 27 February 2020
(This article belongs to the Special Issue Biofilm Implant Related Infections)
The present study assesses the in vitro antibiofilm potential activity of extracts of wild Allium ursinum and Allium oschaninii. The active ingredients of the extracts were obtained with a technique named Naviglio (rapid solid–liquid dynamic extraction, RSLDE) which is based on an innovative and green solid–liquid extraction methodology. The extracts were tested against models of mono- and polymicrobial biofilm structures of clinically antibiotic-resistant pathogens, Klebsiella pneumoniae ATCC 10031 and Candida albicans ATCC 90028. Biofilms were studied using a static and a dynamic model (microtiter plates and a CDC reactor) on three different surfaces reproducing what happens on implantable medical devices. Antimicrobic activities were determined through minimum inhibitory concentration (MIC), while antibiofilm activity was assessed by minimum biofilm eradication concentration (MBEC) using a crystal violet (CV) biofilm assay and colony forming unit (CFU) counts. Results showed that both Allium extracts eradicated biofilms of the tested microorganisms well; biofilms on Teflon were more susceptible to extracts than those on polypropylene and polycarbonate, suggesting that when grown on a complex substrate, biofilms may be more tolerant to antibiotics. Our data provide significant advances on antibiotic susceptibility testing of biofilms grown on biologically relevant materials for future in vitro and in vivo applications. View Full-Text
Keywords: polymicrobial biofilm; CDC biofilm reactor; Allium extracts; Klebsiella pneumoniae; Candida albicans polymicrobial biofilm; CDC biofilm reactor; Allium extracts; Klebsiella pneumoniae; Candida albicans
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MDPI and ACS Style

Galdiero, E.; Di Onofrio, V.; Maione, A.; Gambino, E.; Gesuele, R.; Menale, B.; Ciaravolo, M.; Carraturo, F.; Guida, M. Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models. Microorganisms 2020, 8, 336. https://doi.org/10.3390/microorganisms8030336

AMA Style

Galdiero E, Di Onofrio V, Maione A, Gambino E, Gesuele R, Menale B, Ciaravolo M, Carraturo F, Guida M. Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models. Microorganisms. 2020; 8(3):336. https://doi.org/10.3390/microorganisms8030336

Chicago/Turabian Style

Galdiero, Emilia; Di Onofrio, Valeria; Maione, Angela; Gambino, Edvige; Gesuele, Renato; Menale, Bruno; Ciaravolo, Martina; Carraturo, Federica; Guida, Marco. 2020. "Allium ursinum and Allium oschaninii against Klebsiella pneumoniae and Candida albicans Mono- and Polymicrobic Biofilms in In Vitro Static and Dynamic Models" Microorganisms 8, no. 3: 336. https://doi.org/10.3390/microorganisms8030336

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