Next Article in Journal
Human Cytomegalovirus (HCMV) Reactivation in the Mammary Gland Induces a Proinflammatory Cytokine Shift in Breast Milk
Next Article in Special Issue
Extensive Therapeutic Drug Monitoring of Colistin in Critically Ill Patients Reveals Undetected Risks
Previous Article in Journal
pH and Phosphate Induced Shifts in Carbon Flow and Microbial Community during Thermophilic Anaerobic Digestion
Previous Article in Special Issue
Gut Microbiota, Antibiotic Therapy and Antimicrobial Resistance: A Narrative Review
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 8
Issue 2
10.3390/microorganisms8020287
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Colistin-Resistant Acinetobacter Baumannii Bacteremia: A Serious Threat for Critically Ill Patients

by
Georgios Papathanakos
1,*,
Ioannis Andrianopoulos
1,
Athanasios Papathanasiou
1,
Efthalia Priavali
2,
Despoina Koulenti
3,4 and
Vasilios Koulouras
1
1
University Hospital of Ioannina, Intensive Care Unit, Stavros Niarchos Avenue, 45500 Ioannina, Greece
2
Department of Microbiology, Medical School, University of Ioannina, 45110, Ioannina, Greece
3
Burns, Trauma and Critical Care Research Centre, UQ Centre for Clinical Research (UQCCR), Faculty of Medicine, The University of Queensland, Brisbane, Herston Campus, Brisbane QLD 4029, Australia
4
2nd Critical Care Department, Attikon University Hospital, Rimini Street, 12463, Athens, Greece
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(2), 287; https://doi.org/10.3390/microorganisms8020287
Submission received: 28 January 2020 / Revised: 17 February 2020 / Accepted: 18 February 2020 / Published: 20 February 2020
(This article belongs to the Special Issue Multidrug-Resistant Pathogens)
Browse Figures
Versions Notes

Abstract

:
The prevalence of acinetobacter baumannii (AB) as a cause of hospital infections has been rising. Unfortunately, emerging colistin resistance limits therapeutic options and affects the outcome. The aim of the study was to confirm our clinically-driven hypothesis that intensive care unit (ICU) patients with AB resistant-to-colistin (ABCoR) bloodstream infection (BSI) develop fulminant septic shock and die. We conducted a 28-month retrospective observational study including all patients developing AB infection on ICU admission or during ICU stay. From 622 screened patients, 31 patients with BSI sepsis were identified. Thirteen (41.9%) patients had ABCoR BSI and 18/31 (58.1%) had colistin-susceptible (ABCoS) BSI. All ABCoR BSI patients died; of them, 69% (9/13) presented with fulminant septic shock and died within the first 3 days from its onset. ABCoR BSI patients compared to ABCoS BSI patients had higher mortality (100% vs. 50%, respectively (p = 0.001)), died sooner (p = 0.006), had lower pH (p = 0.004) and higher lactate on ICU admission (p = 0.0001), and had higher APACHE II (p = 0.01) and Charlson Comorbidity Index scores (p = 0.044). In conclusion, we documented that critically ill patients with ABCoR BSI exhibit fulminant septic shock with excessive mortality. Our results highlight the emerging clinical problem of AB colistin resistance among ICU patients.

1. Introduction

Acinetobacter baumannii (AB) is an opportunistic gram-negative with a tendency to colonize the hospital environment: bed rails, floors, ventilator pads, supply carts and infusion pumps in the Intensive Care Unit (ICU) [1,2]. It has the remarkable capacities to survive in dry conditions for a long period of time, to be resistant to disinfectants and form biofilm on abiotic surfaces [3], and also to develop resistance to antibiotics. It predominantly infects critically ill hospitalized patients and it is a frequent cause of nosocomial infections, mainly ventilator-associated pneumonia and bloodstream infection [4].
Although once thought to be benign and previously regarded as an organism of questionable pathogenicity, over the past several years AB has been shown to have increasing resistance to antibiotics affecting both empirical and guided treatment [3]. Today, most AB strains isolated in ICUs are not only resistant to b-lactams and fluroquinolones, but also to carbapenems, aminoglycosides, and possibly to all standard antimicrobial agents, except colistin or tigecycline [5,6,7,8,9,10]. The clinical manifestations of bacteremia of this strain of AB, known as extensively drug-resistant (XDR AB), ranges from benign transient bacteremia to fulminant septic shock [11]. XDR AB bacteremia is accompanied with excess mortality [12,13], excess length of ICU hospitalization, prolonged hospital stay [14], and higher overall costs [15,16]. XDR AB has been reported worldwide as a major cause of healthcare-associated infections and nosocomial outbreaks [17], and is now recognized as one of the most difficult hospital pathogens to be treated and controlled and is considered a global threat in the health-care setting [18].
This increasing worldwide prevalence of AB drug resistance has made colistin an important therapeutic option for XDR AB. Unfortunately, resistance to colistin has been reported among AB clinical strains [19,20,21,22,23,24].
Despite the potential magnitude of the problem, data regarding the clinical, microbiological, and molecular characteristics of ABCoR infections remain very limited [25]. Most studies include patients hospitalized in several wards and not especially in an ICU [25,26,27], while in a recent prospective observational study mortality rate for patients with ABCoR infection in an ICU setting was found to be as high as 85% [28].
Over the last year, a colistin resistant XDR AB strain has emerged in our ICU. Our clinically based observation suggested that patients with bloodstream infection (BSI) of this strain have a high mortality by developing a fulminant course of septic shock. The aim of our study was to confirm the above-mentioned observation by investigating the impact of XDR AB BSIs on the mortality of ICU patients and to compare the characteristics of ABCoR BSIs with the colistin-sensitive (ABCoS) ones.

2. Materials and Methods

The study was conducted in accordance with the Declaration of Helsinki, and the protocol was approved by the Ethics Committee of the University Hospital of Ioannina (reference number 1092). Informed consent was waived because of the observational retrospective nature of the study.
This is a retrospective observational study of 28 months duration conducted in a 14-bed ICU of a university hospital. During the study period (June 2017 to October 2019), all cultures obtained from the ICU patients were screened and all patients that were admitted to ICU with or developed AB infection were included in the study.
For every patient with established AB infection demographic characteristics, medical history, cause of ICU admission, and APACHE II score were recorded [29]. Sequential (sepsis-related) organ failure assessment (SOFA) score was calculated at ICU admission, 24 h before and on the day that infection was documented (infection onset) [30]. Patients with cancer under treatment, on chronic corticosteroid treatment, with autoimmune disease and/or immunosuppressant treatment, cirrhosis, and end-stage renal disease, were considered to be patients with an impaired immune response. For patients with positive blood cultures, Charlson Comorbidity Index score was calculated as well [31]. During the study period, for all patients with AB infection, we recorded baseline biochemical parameters, risk factors, empirical antibiotic therapy, days from hospital admission to sepsis, days from ICU admission to sepsis, days from admission to positive blood cultures, days from hospital admission to shock, and time from sepsis to shock development. Clinical complications were recorded as well and defined according to international guidelines. These included the development of acute respiratory distress syndrome (ARDS), acute kidney injury (AKI) [32], need for renal replacement therapy (RRT), septic myocardiopathy, thrombocytopenia (count of platelets less than 100,000/μL), or liver dysfunction [33]. For all documented infection by AB, antibiograms were also obtained and analyzed to identify the susceptibility of AB to the antibiotics.
Susceptibility of antibiotics to AB was assessed by VITEK 2 system and susceptibility to colistin was assessed by broth microdilution with resistant defined as having a colistin minimum inhibitory concentration (MIC) breakpoint >2 mg/L. Vitek 2 system (bioMerieux, Marcy I ’Etoile, France) is a fully automated system utilizing a fluorogenic methodology for organism identification and a turbidimetric method for susceptibility testing, using a 64 well card that is barcoded with information on card type, expiration date, lot number, and unique card identification number. VITEK 2 antimicrobial susceptibility testing has shown to have a high degree of agreement with standard methods for determining the MIC of antimicrobials, with a gain-of-time of hours to days and improved reproducibility [34,35]. XDR AB were defined as those isolates being non-susceptible to at least one agent in all but two or fewer antibiotic classes. Appropriate treatment was defined by the utilization of at least one susceptible antibiotic against AB during the infection period.
Definitions: Definitions of sepsis and septic shock were based on recent SEPSIS-3 international guidelines [36]. Sepsis is a clinical syndrome of life-threatening organ dysfunction caused by dysregulated host response to infection and defined as suspected or documented infection and an acute increase of ≥2 SOFA points. Septic shock was identified when a clinical construct of sepsis with persisting hypotension, requiring vasopressor therapy to elevate mean arterial pressure ≥65 mm Hg and lactate >2 mmol/L despite adequate fluid resuscitation was present [36]. AB infection was defined as the clinical manifestation of infection, which could be microbiologically confirmed by the isolation of the specific pathogen in cultured material. Ventilator associated pneumonia was defined as pneumonia occurring later than 48 h post endotracheal intubation [37].
Mortality: Sepsis related deaths were categorized as deaths directly linked to AB infection and its implications (sepsis, septic shock, and multiple organ dysfunction syndrome-MODS—due to septic shock).
Statistical analysis: Numerical variables were presented as the mean ± standard deviation, or median, min-max values when appropriate. Comparisons of continuous variables were performed by Mann-Whitney test for non-normally distributed variables, whereas w2 tests were used for categorical variables. The assumption concerning the proportionality of hazards was graphically assessed and met for all covariates of Kaplan-Meier survival plots of matched groups were constructed and compared by using the log-rank test. Significance was defined as a p-value < 0.05 in all cases. SPSS® 21.0 (Chicago, IL, USA) was used for all analyses.

3. Results

During the study period, 44 (mean age 65.3 ± 17.6 years) out of a total of 622 patients were identified with infection by AΒ. The flowchart of the study population is shown in Figure 1.

3.1. Patient Characteristics

The baseline characteristics of these patients and causes of ICU admission are shown in Table 1, while AΒ infection characteristics are shown in Table 2. Out of 44 patients, 39 (88.6%) developed AB sepsis. Of these, in 28/39 (71,8%) it occurred during ICU hospitalization, while in the other 11/39 (28.2%) patients, AB sepsis itself was the cause of ICU admission. Twenty-nine patients (65.9%) fulfilled the criteria for septic shock (Table 2).
Twenty-one out of 44 infections were due to a colistin-resistant AΒ strain. Six of the above AB infections were pan-resistant (PDR), featuring intermediate sensitivity to tigecycline only. Mortality was high in this group, as 18/21 (85.7%) died during their admission. Data on previous antibiotic usage were available in 18/21 of these patients. Only 6/18 (33%) of these patients had at some point prior to their infection received colistin.
In addition, 15/40 (37.5%) patients that developed sepsis died in less than 72 h, featuring septic shock and MODS. This was irrespective of appropriate antibiotic treatment and almost all of these patients had a high SOFA score 24 h before the onset of sepsis (10.5 ± 3.8 h), indicating that there was already significant organ dysfunction in place.

3.2. Patients with Positive Blood Cultures Due to AΒ

Among the 44 patients, 31 AΒ were identified with sepsis due to BSI by (31/44, 70.5%). Of these, 26 were primary- or catheter-related BSI, and in the other five positive blood cultures were associated with pneumonia (three patients) and central nervous system infections (two patients) (Figure 1).
Between the 31 septic patients with positive blood cultures, AΒ was colistin-resistant in 13 patients. All 13 patients developed septic shock. In the other 18 patients, AΒ was susceptible to colistin. In these 18 patients, septic shock was evident in 14 out of 18 (78%). Univariate analysis showed that patients with AB resistant to colistin tend to be older, with higher APACHE II and Charlson Comorbidity Index scores, lower levels of pH and higher level of lactate on admission and maximum levels of lactate during hospitalization (Table 3). Additionally, the duration of shock and the duration from the onset of sepsis to death were statistically significant between patients with AB resistant to colistin and AB susceptible to colistin (Table 3). Multivariate analysis did not reveal any statistical significance for any of the above-mentioned parameters probably due to the small number of cases.

3.3. Patients’ Outcome

A total of 29 deaths were recorded (mortality of all causes 29/44, 65.9%). Of these, 16 deaths (16/29, 55.1% of all deaths) were directly related to sepsis from AB (sepsis related mortality 16/44, 36.4%).
Patients with BSI by AB and septic shock (n = 27) were further divided into two groups based on the susceptibility of AB to colistin: Group A, patients infected by ABCoR (n = 13) and group B, patients infected by ABCoS (n = 14).
Septic shock was documented in all patients of group A within the first hours of the onset of sepsis. All group A patients died (13/13, 100%, sepsis related deaths 9/13, 70%). In group B, patients’ mortality rate was significantly lower compared to group A (6/14, 42.8%, sepsis related deaths 4/6, 66.6%, p = 0.026). Time from the onset of sepsis till death for group A patients was shorter compared to group B patients (3 days—min 1 to 9 days and 6 days—min 3 to 25 days), (p = 0.005). The estimated 28-day Kaplan-Meier survival curve for total mortality is shown in Figure 2. The Kaplan-Meier survival curve for total mortality diverged at the 3rd day, suggesting that the significant difference in mortality is evident within the first three days from the onset of septic shock.

4. Discussion

Our study showed that septic patients with BSIs from XDR ABCoR have a significantly higher mortality when compared to septic patients with XDR ABCoS BSIs. Interestingly, patients with ABCoR BSIs had a fulminant course, with rapid progress to septic shock and very high sepsis-related mortality, dying in just a few days from the onset of sepsis.
Our patients with AB BSIs had a high overall mortality rate of 64.5%, and colistin resistance itself seemed to impact mortality, as all patient with ABCoR died. This is in accordance with previous studies that reported mortality from AB BSIs ranging from 52.3–84.7% depending on the study population [27,38,39,40,41]. In our study, all the patients with ABCoR BSI eventually died. In the literature we found only three studies demonstrating that ABCoR infection, in general, has a high mortality rate and that colistin resistance is an independent risk factor for mortality [25,42,43]. In a recent retrospective multi-centric study by Aydin et al., where healthcare-associated infections were investigated in 20 tertiary care centers, ABCoR was present in only 2.1% of the cases (n = 9 patients). Nevertheless, CoR was significantly associated with increased fatality rate (55,7%) [42]. In the retrospective study by Lertsrisatit et al., among 19 patients with ABCoR isolates related to pneumonia, septicemia of unknown origin, and cholangitis, the 30-day mortality was as high as 70.6% [43]. On the other hand, the single-center retrospective US study of Qureshi et al. reported a 30-day all-cause mortality rate due to ABCoR infection (mostly VAP) of only 30% [25]. However, this study had only 20 patients with ABCoR infection or colonization and lacked a comparison group with colistin-susceptible AB cases.
To our knowledge there are only two studies investigating the impact of either ABCoR infection or bacteremia on the mortality of patients pooled exclusively from an ICU population and our findings are in full agreement with the results of these studies: In the recently published prospective observational study from Mantzarlis et al., in ICU patients who required mechanical ventilation for >48 h during a 36-month period, the mortality rate among 20 patients with ABCoR infection was 85% vs. 39% of 57 patients for the ABCoS group [28]. Additionally, in the retrospective study of Katsiari et al., 16 out of 29 patients (55.1%) of an ICU population with ABCoR BSI died [44].
Our results are in contrast with those of a secondary analysis of a randomized controlled trial of patients with severe infections due to carbapenem-resistant AB, where colistin resistance was associated with a significantly lower 28-day mortality of 42.3% [45]. This different result may be explained by the different patient populations chosen for the conduction of the study (i.e., secondary analysis, fewer patients on mechanical ventilation with higher baseline functional capacity).
Our study also revealed that almost three out of four patients with ABCoR BSI presented with fulminant septic shock with a significantly increased mortality within the first 3 days from its onset. We consider this a very important finding since it is first one to reach to the above conclusion with a statistical significance. There are only two other studies that have addressed this [28,44]. The first study was that of Mantzarlis et al., where patients infected with ABCoR died sooner after the onset of the infection in comparison to patients infected with ABCoS (no statement about statistical significance) [28]. In the study of Katsiari et al., authors divided patients with AB bacteremia into three groups, according to their ICU outcome; those who survived, those that died early (within 48 h, i.e., fulminant sepsis) and those that died later (>48 h) after the onset of bacteremia. In this study, ABCoR was higher in the patient group with fulminant sepsis, though this did not reach statistical significance probably due to the small patient sample size [44]. Authors of both studies assume that the fact that ABCoR patients died sooner in comparison to patients infected with ABCoS may be explained by the virulence of ABCoR strains and perhaps the lack of appropriate therapy.
According to the extensive review by Wong et al., in the case of AB infection, antibiotic resistance itself drives the outcome of the patients [46]. Inappropriate antimicrobial therapy seems to be an independent risk factor for mortality not only in XDR AB bacteremia, as already stated [27,41,47], but also in multidrug-resistant AB or carbapenem-resistant AB bacteremia [16,48,49,50,51,52,53,54,55]. Nevertheless, our study failed to demonstrate any statistically significant difference between the two compared groups, possibly due to the small number of patients.
Virulence is probably an important factor. Although most of the experimental studies report a decreased virulence of ABCoR [56], our study supports the hypothesis that in vitro results do not necessarily correlate with the in vivo outcome [57]. On the other hand, it has been experimentally shown that even ABCoR clinical strains recovered during colistin therapy progressively increase their virulence under oxidative stress [57], and when macrophages-, complement-, or neutrophil-related host defense mechanisms are depleted, even avirulent AB strains trigger a lethal lipopolysaccharide Toll-like receptor 4 (TLR-4) mediated sepsis response [46,58]. We assume that this is in part the case with the majority of our patients exhibiting septic shock: in those patients, AB strains escaped innate immune clearance and initiated a TLR4 mediated sepsis. Moreover, it has already been mentioned that the presence of septic shock is indeed a proven independent risk factor of mortality due to XDR AB bacteremia. This is also the case for carbapenem-resistant AB, as confirmed by a recent systemic review and meta-analysis [48]. Unfortunately, we cannot be sure if infection with ABCoR is per se a mortality factor or which host factors, such as underlying diseases, determined the clinical outcome of our patients.
On the other hand, taking into consideration that the presence of septic shock is an important factor in the outcome of XDR AB BSI, one could speculate that in our patients, once host dysfunction became apparent, shifting the balance against the infection was very difficult despite presumed appropriate antibiotic coverage. In our study, a significant proportion of patients that developed sepsis died in less than 72 h, due to septic shock and MODS. This was irrespective of appropriate antibiotic treatment and almost all of these patients had a high SOFA score 24 h before the onset of sepsis, indicating that there was already significant organ dysfunction in place. Our hypothesis is that once organ dysfunction is established in such infections, the Rubicon is crossed in terms of the outcome and appropriate antibiotic treatment by itself fails to prevent mortality. Katsiari et al. [44], reached a similar conclusion, as patients with fulminant sepsis had more severe organ dysfunction and more resistant pathogens, though they failed to show statistical significance probably due to small patient numbers.
Finally, it must be pointed out that patients with ABCoR sepsis had higher Charlson Comorbidity Index score as well as APACHE II score on admission which could affect the outcome, though there was no difference in SOFA score and SOFA change among the two groups. It could be expected that sicker patients have higher mortality, and that APACHE II and Charlson Comorbidity Index scores are risk factors for multidrug resistant infections.
Our study has some limitations that are noteworthy. The most important of these involves the retrospective observational single-center design, and that risk factors for mortality might have been unequally distributed between the two groups. Unmeasured confounders might have also been in place, which in combination with the relatively small patients’ number, renders difficult to differentiate mortality directly attributable to ABCoR BSI from those as a result of comorbidities. Moreover, identification of AB involved only conventional cultures and no molecular typing was performed to characterize the particular AB strains.
On the other hand, to the best of our knowledge, this is the first cohort study investigating the impact of ABCoR BSI on the mortality of patients pooled exclusively from an ICU population. Colistin resistance is associated with higher comorbidity and mortality. It remains debatable whether the cause for high mortality is due to the fact that the more severely ill patients have a higher chance of acquiring an ABCoR BSI or the bacteremia itself. What is undebatable, though, is the fact that when patients develop sepsis due to this XDR pathogen, they have a very poor survival.

5. Conclusions

ICU patients with ABCoR BSI infections have excessive mortality and they often develop a fulminant form of sepsis that rapidly progresses to shock and eventually death.

Author Contributions

Conceptualization, G.P., I.A., A.P, D.K. and V.K.; methodology, I.A., A.P. and V.K.; software, A.P.; investigation, I.A. and E.P.; writing—original draft preparation, G.P., I.A., A.P, D.K. and V.K.; writing—review and editing, G.P., I.A., A.P, D.K. and V.K.; visualization, G.P., I.A., A.P, D.K. and V.K.; supervision, D.K. and V.K. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Acknowledgments

Vassiliki Stamati for technical support. Antigoni Manou for administrative support.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Thom, K.A.; Johnson, J.K.; Lee, M.S.; Harris, A.D. Environmental contamination because of multidrug-resistant Acinetobacter baumannii surrounding colonized or infected patients. Am. J. Infect. Control. 2011, 39, 711–715. [Google Scholar] [CrossRef] [Green Version]
  2. Rosa, R.M.; DePascale, D.; Cleary, T.; Fajardo-Aquino, Y.; Kett, D.H.; Munoz-Price, L.S. Differential environmental contamination with Acinetobacter baumannii based on the anatomic source of colonization. Am. J. Infect. Control. 2014, 42, 755–757. [Google Scholar] [CrossRef] [PubMed]
  3. Harding, C.M.; Hennon, S.W.; Feldman, M.F. Uncovering the mechanisms of Acinetobacter baumannii virulence. Nat. Rev. Genet. 2017, 16, 91–102. [Google Scholar] [CrossRef] [PubMed]
  4. Maraki, S.; Mantadakis, E.; Mavromanolaki, V.E.; Kofteridis, D.; Samonis, G. A 5-year Surveillance Study on Antimicrobial Resistance of Acinetobacter baumannii Clinical Isolates from a Tertiary Greek Hospital. Infect. Chemother. 2016, 48, 190–198. [Google Scholar] [CrossRef] [PubMed]
  5. Kuo, S.-C.; Chang, S.-C.; Wang, H.-Y.; Lai, J.-F.; Chen, P.-C.; Shiau, Y.-R.; Huang, I.-W.; Lauderdale, T.-L. Emergence of extensively drug-resistant Acinetobacter baumannii complex over 10 years: Nationwide data from the Taiwan Surveillance of Antimicrobial Resistance (TSAR) program. BMC Infect. Dis. 2012, 12, 200. [Google Scholar] [CrossRef] [Green Version]
  6. Su, C.-H.; Wang, J.-T.; Hsiung, C.A.; Chien, L.-J.; Chi, C.-L.; Yu, H.-T.; Chang, F.-Y.; Chang, S.-C. Increase of Carbapenem-Resistant Acinetobacter baumannii Infection in Acute Care Hospitals in Taiwan: Association with Hospital Antimicrobial Usage. PLoS ONE 2012, 7, 37788. [Google Scholar] [CrossRef] [Green Version]
  7. Perez, F.; Hujer, A.M.; Hujer, K.M.; Decker, B.K.; Rather, P.N.; Bonomo, R.A. Global Challenge of Multidrug-Resistant Acinetobacter baumannii. Antimicrob. Agents Chemother 2007, 51, 3471–3484. [Google Scholar] [CrossRef] [Green Version]
  8. Falagas, M.E.; Karveli, E.A.; Siempos, I.I.; Vardakas, K.Z. Acinetobacter infections: A growing threat for critically ill patients. Epidemiol. Infect. 2007, 136, 1009–1019. [Google Scholar] [CrossRef]
  9. Maragakis, L.L.; Perl, T.M.; Eliopoulos, G.M. Antimicrobial Resistance: Acinetobacter baumannii: Epidemiology, Antimicrobial Resistance, and Treatment Options. Clin. Infect. Dis. 2008, 46, 1254–1263. [Google Scholar] [CrossRef] [Green Version]
  10. Jean, S.-S.; Hsueh, P.-R.; Lee, W.-S.; Chang, H.-T.; Chou, M.-Y.; Chen, I.-S.; Wang, J.-H.; Lin, C.-F.; Shyr, J.-M.; Ko, W.-C.; et al. Nationwide surveillance of antimicrobial resistance among non-fermentative Gram-negative bacteria in Intensive Care Units in Taiwan: SMART programme data 2005. Int. J. Antimicrob. Agents 2009, 33, 266–271. [Google Scholar] [CrossRef]
  11. Ballouz, T.; Aridi, J.; Afif, C.; Irani, J.; Lakis, C.; Nasreddine, R.; Azar, E. Risk Factors, Clinical Presentation, and Outcome of Acinetobacter baumannii Bacteremia. Front. Microbiol. 2017, 7, 28. [Google Scholar] [CrossRef] [PubMed]
  12. Lee, H.-Y.; Chen, C.-L.; Wu, S.-R.; Huang, C.-W.; Chiu, C.-H. Risk Factors and Outcome Analysis of Acinetobacter baumannii Complex Bacteremia in Critical Patients. Crit. Care Med. 2014, 42, 1081–1088. [Google Scholar] [CrossRef] [PubMed]
  13. Liu, C.P.; Shih, S.-C.; Wang, N.-Y.; Wu, A.Y.; Sun, F.-J.; Chow, S.-F.; Chen, T.-L.; Yan, T.-R. Risk factors of mortality in patients with carbapenem-resistant Acinetobacter baumannii bacteremia. J. Microbiol. Immunol. Infect. 2016, 49, 934–940. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Sunenshine, R.H.; Wright, M.-O.; Maragakis, L.L.; Harris, A.D.; Song, X.; Hebden, J.; Cosgrove, S.E.; Anderson, A.; Carnell, J.; Jernigan, D.B.; et al. Multidrug-resistant Acinetobacter Infection Mortality Rate and Length of Hospitalization. Emerg. Infect. Dis. 2007, 13, 97–103. [Google Scholar] [CrossRef] [PubMed]
  15. Kim, S.Y.; Jung, J.Y.; Kang, Y.A.; Lim, J.E.; Kim, E.Y.; Lee, S.K.; Park, S.C.; Chung, K.S.; Park, B.H.; Kim, Y.S.; et al. Risk Factors for Occurrence and 30-Day Mortality for Carbapenem-Resistant Acinetobacter baumannii Bacteremia in an Intensive Care Unit. J. Korean Med Sci. 2012, 27, 939–947. [Google Scholar] [CrossRef] [Green Version]
  16. Kim, Y.J.; Kim, S.I.; Hong, K.-W.; Kim, Y.R.; Park, Y.J.; Kang, M.-W. Risk Factors for Mortality in Patients with Carbapenem-Resistant Acinetobacter baumannii Bacteremia: Impact of Appropriate Antimicrobial Therapy. J. Korean Med Sci. 2012, 27, 471–475. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  17. Nowak, J.; Zander, E.; Stefanik, D.; Higgins, P.G.; Roca, I.; Vila, J.; McConnell, M.J.; Cisneros, J.M.; Seifert, H. MagicBullet Working Group WP4 High incidence of pandrug-resistant Acinetobacter baumannii isolates collected from patients with ventilator-associated pneumonia in Greece, Italy and Spain as part of the MagicBullet clinical trial. J. Antimicrob. Chemother. 2017, 72, 3277–3282. [Google Scholar] [CrossRef]
  18. Mavroidi, A.; Katsiari, M.; Palla, E.; Likousi, S.; Roussou, Z.; Nikolaou, C.; Platsouka, E.D. Investigation of Extensively Drug-Resistant blaOXA-23-Producing Acinetobacter baumannii Spread in a Greek Hospital. Microb. Drug Resist. 2017, 23, 488–493. [Google Scholar] [CrossRef]
  19. Lesho, E.; Yoon, E.-J.; Mc Gann, P.; Snesrud, E.; Kwak, Y.; Milillo, M.; Onmus-Leone, F.; Preston, L.; Clair, K.S.; Nikolich, M.; et al. Emergence of Colistin-Resistance in Extremely Drug-Resistant Acinetobacter baumannii Containing a Novel pmrCAB Operon During Colistin Therapy of Wound Infections. J. Infect. Dis. 2013, 208, 1142–1151. [Google Scholar] [CrossRef] [Green Version]
  20. López-Rojas, R.; McConnell, M.J.; Jiménez-Mejías, M.E.; Dominguez-Herrera, J.; Fernández-Cuenca, F.; Pachón, J. Colistin Resistance in a Clinical Acinetobacter baumannii Strain Appearing after Colistin Treatment: Effect on Virulence and Bacterial Fitness. Antimicrob. Agents Chemother. 2013, 57, 4587–4589. [Google Scholar] [CrossRef] [Green Version]
  21. Pelletier, M.R.; Casella, L.G.; Jones, J.W.; Adams, M.D.; Zurawski, D.; Hazlett, K.R.O.; Doi, Y.; Ernst, R.K. Unique Structural Modifications Are Present in the Lipopolysaccharide from Colistin-Resistant Strains of Acinetobacter baumannii. Antimicrob. Agents Chemother. 2013, 57, 4831–4840. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  22. O’Hara, J.A.; Ambe, L.A.; Casella, L.G.; Townsend, B.M.; Pelletier, M.R.; Ernst, R.K.; Shanks, R.M.Q.; Doi, Y. Activities of Vancomycin-Containing Regimens against Colistin-Resistant Acinetobacter baumannii Clinical Strains. Antimicrob. Agents Chemother. 2013, 57, 2103–2108. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Pogue, J.M.; Cohen, D.A.; Marchaim, D. Editorial commentary: Polymyxin-resistant Acinetobacter baumannii: Urgent action needed. Clin. Infect. Dis. 2015, 60, 1304–1307. [Google Scholar] [PubMed] [Green Version]
  24. Cai, Y.; Chai, D.; Wang, R.; Liang, B.; Bai, N. Colistin resistance of Acinetobacter baumannii: Clinical reports, mechanisms and antimicrobial strategies. J. Antimicrob. Chemother. 2012, 67, 1607–1615. [Google Scholar] [CrossRef] [PubMed]
  25. Qureshi, Z.A.; Hittle, L.E.; O’Hara, J.A.; Rivera, J.I.; Syed, A.; Shields, R.K.; Pasculle, A.W.; Ernst, R.K.; Doi, Y. Colistin-resistant Acinetobacter baumannii: Beyond carbapenem resistance. Clin. Infect. Dis. 2015, 60, 1295–1303. [Google Scholar] [CrossRef] [Green Version]
  26. Matthaiou, D.; Michalopoulos, A.; Rafailidis, P.I.; Karageorgopoulos, D.; Papaioannou, V.; Ntani, G.; Samonis, G.; Falagas, M.E. Risk factors associated with the isolation of colistin-resistant Gram-negative bacteria: A matched case-control study. Crit. Care Med. 2008, 36, 807–811. [Google Scholar] [CrossRef] [PubMed]
  27. Ng, T.M.; Teng, C.B.; Lye, D.; Apisarnthanarak, A. A Multicenter Case-Case Control Study for Risk Factors and Outcomes of Extensively Drug-Resistant Acinetobacter baumannii Bacteremia. Infect. Control. Hosp. Epidemiol. 2014, 35, 49–55. [Google Scholar] [CrossRef] [PubMed]
  28. Mantzarlis, K.; Makris, D.; Zakynthinos, E. Risk factors for the first episode of Acinetobacter baumannii resistant to colistin infection and outcome in critically ill patients. J. Med Microbiol. 2020, 69, 35–40. [Google Scholar] [CrossRef]
  29. A Knaus, W.; A Draper, E.; Wagner, D.P.; E Zimmerman, J. APACHE II: A severity of disease classification system. Crit. Care Med. 1985, 13, 818–829. [Google Scholar] [CrossRef]
  30. Vincent, J.-L.; De Mendonça, A.; Cantraine, F.; Moreno, R.; Takala, J.; Suter, P.M.; Sprung, C.L.; Colardyn, F.; Blecher, S. Use of the SOFA score to assess the incidence of organ dysfunction/failure in intensive care units. Crit. Care Med. 1998, 26, 1793–1800. [Google Scholar] [CrossRef]
  31. Charlson, M.E.; Pompei, P.; Ales, K.L.; MacKenzie, C. A new method of classifying prognostic comorbidity in longitudinal studies: Development and validation. J. Chronic Dis. 1987, 40, 373–383. [Google Scholar] [CrossRef]
  32. Kellum, J.; Lameire, N.; Aspelin, P.; Barsoum, R.S.; Burdmann, E.A.; Goldstein, S.L.; Herzog, C.A.; Joannidis, M.; Kribben, A.; Levey, A.S.; et al. KDIGO clinical practice guideline for acute kidney injury. Kidney Int. Suppl. 2012, 2, 1–138. [Google Scholar]
  33. Dellinger, R.P.; Levy, M.M.; Rhodes, A.; Annane, D.; Gerlach, H.; Opal, S.M.; Sevransky, J.E.; Sprung, C.L.; Douglas, I.S.; The Surviving Sepsis Campaign Guidelines Committee including The Pediatric Subgroup; et al. Surviving Sepsis Campaign: International Guidelines for Management of Severe Sepsis and Septic Shock, 2012. Intensiv. Care Med. 2013, 39, 165–228. [Google Scholar] [CrossRef]
  34. Choi, S.-H.; Cho, E.B.; Chung, J.W.; Lee, M.-K. Changes in the early mortality of adult patients with carbapenem-resistant Acinetobacter baumannii bacteremia during 11 years at an academic medical center. J. Infect. Chemother. 2019, 25, 6–11. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Bitew, A.; Molalign, T.; Chanie, M. Species distribution and antibiotic susceptibility profile of bacterial uropathogens among patients complaining urinary tract infections. BMC Infect. Dis. 2017, 17, 654. [Google Scholar] [CrossRef] [Green Version]
  36. Singer, M.; Deutschman, C.S.; Seymour, C.W.; Shankar-Hari, M.; Annane, D.; Bauer, M.; Bellomo, R.; Bernard, G.R.; Chiche, J.-D.; Coopersmith, C.M.; et al. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA 2016, 315, 801–810. [Google Scholar] [CrossRef]
  37. Kalil, A.C.; Metersky, M.L.; Klompas, M.; Muscedere, J.; Sweeney, D.A.; Palmer, L.B.; Napolitano, L.M.; O’Grady, N.P.; Bartlett, J.G.; Carratalà, J.; et al. Management of Adults With Hospital-acquired and Ventilator-associated Pneumonia: 2016 Clinical Practice Guidelines by the Infectious Diseases Society of America and the American Thoracic Society. Clin. Infect. Dis. 2016, 63, e61–e111. [Google Scholar] [CrossRef]
  38. Fu, Q.; Ye, H.; Liu, S. Risk factors for extensive drug-resistance and mortality in geriatric inpatients with bacteremia caused by Acinetobacter baumannii. Am. J. Infect. Control. 2015, 43, 857–860. [Google Scholar] [CrossRef]
  39. Tseng, Y.-C.; Wang, J.-T.; Wu, F.-L.L.; Chen, Y.-C.; Chie, W.-C.; Chang, S.-C. Prognosis of adult patients with bacteremia caused by extensively resistant Acinetobacter baumannii. Diagn. Microbiol. Infect. Dis. 2007, 59, 181–190. [Google Scholar] [CrossRef]
  40. Batirel, A.; Balkan, I.I.; Karabay, O.; Agalar, C.; Akalin, S.; Alici, O.; Alp, E.; Altay, F.A.; Altin, N.; Arslan, F.; et al. Comparison of colistin–carbapenem, colistin–sulbactam, and colistin plus other antibacterial agents for the treatment of extremely drug-resistant Acinetobacter baumannii bloodstream infections. Eur. J. Clin. Microbiol. Infect. Dis. 2014, 33, 1311–1322. [Google Scholar] [CrossRef]
  41. Freire, M.; Garcia, D.D.O.; Garcia, C.; Bueno, M.C.; Camargo, C.; Magri, A.K.; Francisco, G.; Reghini, R.; Vieira, M.; Ibrahim, K.; et al. Bloodstream infection caused by extensively drug-resistant Acinetobacter baumannii in cancer patients: High mortality associated with delayed treatment rather than with the degree of neutropenia. Clin. Microbiol. Infect. 2016, 22, 352–358. [Google Scholar] [CrossRef]
  42. Aydın, M.; Ergonul, O.; Azap, A.; Bilgin, H.; Aydın, G.; Çavuş, S.A.; Demiroğlu, Y.Z.; Çalışkan, H.E.; Memikoğlu, O.; Menekşe, Ş.; et al. Rapid emergence of colistin resistance and its impact on fatality among healthcare-associated infections. J. Hosp. Infect. 2018, 98, 260–263. [Google Scholar] [CrossRef]
  43. Lertsrisatit, Y.; Santimaleeworagun, W.; Thunyaharn, S.; Traipattanakul, J. In vitro activity of colistin mono- and combination therapy against colistin-resistant Acinetobacter baumannii, mechanism of resistance, and clinical outcomes of patients infected with colistin-resistant A. baumannii at a Thai university hospital. Infect. Drug Resist. 2017, 10, 437–443. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Katsiari, M.; Mavroidi, A.; Platsouka, E.; Nikolaou, C. Extensively drug-resistant Acinetobacter baumannii bacteraemia in a multidisciplinary intensive care unit during a 6-year period: Risk factors for fulminant sepsis. J. Glob. Antimicrob. Resist. 2018, 14, 51–57. [Google Scholar] [CrossRef] [PubMed]
  45. Dickstein, Y.; Lellouche, J.; Amar, M.B.D.; Schwartz, D.; Nutman, A.; Daitch, V.; Yahav, D.; Leibovici, L.; Skiada, A.; Antoniadou, A.; et al. Treatment Outcomes of Colistin- and Carbapenem-resistant Acinetobacter baumannii Infections: An Exploratory Subgroup Analysis of a Randomized Clinical Trial. Clin. Infect. Dis. 2018, 69, 769–776. [Google Scholar] [CrossRef] [PubMed]
  46. Wong, D.; Nielsen, T.B.; Bonomo, R.A.; Pantapalangkoor, P.; Luna, B.; Spellberg, B. Clinical and Pathophysiological Overview ofAcinetobacterInfections: A Century of Challenges. Clin. Microbiol. Rev. 2016, 30, 409–447. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  47. Song, J.Y.; Cheong, H.J.; Choi, W.S.; Heo, J.Y.; Noh, J.Y.; Kim, W.J. Clinical and microbiological characterization of carbapenem-resistant Acinetobacter baumannii bloodstream infections. J. Med. Microbiol. 2011, 60, 605–611. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  48. Du, X.; Xu, X.; Yao, J.; Deng, K.; Chen, S.; Shen, Z.; Yang, L.; Feng, G. Predictors of mortality in patients infected with carbapenem-resistant Acinetobacter baumannii: A systematic review and meta-analysis. Am. J. Infect. Control. 2019, 47, 1140–1145. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  49. Shorr, A.F.; Zilberberg, M.D.; Micek, S.T.; Kollef, M.H. Predictors of hospital mortality among septic ICU patients with Acinetobacter spp.bacteremia: A cohort study. BMC Infect. Dis. 2014, 14, 572. [Google Scholar] [CrossRef]
  50. Huang, S.-T.; Chiang, M.-C.; Kuo, S.-C.; Lee, Y.-T.; Chiang, T.-H.; Yang, S.-P.; Yin, T.-; Chen, T.-L.; Fung, C.-P. Risk factors and clinical outcomes of patients with carbapenem-resistant Acinetobacter baumannii bacteremia. J. Microbiol. Immunol. Infect. 2012, 45, 356–362. [Google Scholar] [CrossRef] [Green Version]
  51. Hernandez-Torres, A.; García-Vázquez, E.; Gómez, J.; Canteras, M.; Ruiz, J.; Yagüe, G. Multidrug and carbapenem-resistant Acinetobacter baumannii infections: Factors associated with mortality. Med. Clínica 2012, 138, 650–655. [Google Scholar] [CrossRef]
  52. Esterly, J.S.; Griffith, M.; Qi, C.; Malczynski, M.; Postelnick, M.J.; Scheetz, M.H. Impact of Carbapenem Resistance and Receipt of Active Antimicrobial Therapy on Clinical Outcomes of Acinetobacter baumannii Bloodstream Infections. Antimicrob. Agents Chemother. 2011, 55, 4844–4849. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Park, K.-H.; Shin, J.-H.; Lee, S.Y.; Kim, S.H.; Jang, M.O.; Kang, S.J.; Jung, S.-I.; Chung, E.-K.; Ko, K.S.; Jang, H.-C. The Clinical Characteristics, Carbapenem Resistance, and Outcome of Acinetobacter Bacteremia According to Genospecies. PLoS ONE 2013, 8, e65026. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Park, S.Y.; Lee, E.J.; Kim, T.; Yu, S.N.; Park, K.-H.; Lee, M.S.; Park, S.Y.; Jeon, M.H.; Kim, T.H.; Choo, E.J.; et al. Early administration of appropriate antimicrobial agents to improve the outcome of carbapenem-resistant Acinetobacter baumannii complex bacteraemic pneumonia. Int. J. Antimicrob. Agents 2018, 51, 407–412. [Google Scholar] [CrossRef] [PubMed]
  55. Lemos, E.; De La Hoz, F.; Einarson, T.; McGhan, W.; Quevedo, E.; Castañeda, C.; Kawai, K. Carbapenem resistance and mortality in patients with Acinetobacter baumannii infection: Systematic review and meta-analysis. Clin. Microbiol. Infect. 2014, 20, 416–423. [Google Scholar] [CrossRef] [Green Version]
  56. Da Silva, G.J.; Domingues, S. Interplay between Colistin Resistance, Virulence and Fitness in Acinetobacter baumannii. Antibiot. 2017, 6, 28. [Google Scholar] [CrossRef] [Green Version]
  57. Jones, C.L.; Singh, S.S.; Alamneh, Y.; Casella, L.G.; Ernst, R.K.; Lesho, E.P.; Waterman, P.E.; Zurawski, D.V. In Vivo Fitness Adaptations of Colistin-Resistant Acinetobacter baumannii Isolates to Oxidative Stress. Antimicrob. Agents Chemother. 2017, 61, e00598-16. [Google Scholar] [CrossRef] [Green Version]
  58. Bruhn, K.W.; Pantapalangkoor, P.; Nielsen, T.; Tan, B.; Junus, J.; Hujer, K.M.; Wright, M.S.; Bonomo, R.A.; Adams, M.D.; Chen, W.; et al. Host fate is rapidly determined by innate effector-microbial interactions during Acinetobacter baumannii bacteremia. J. Infect. Dis. 2014, 211, 1296–1305. [Google Scholar] [CrossRef] [Green Version]
Microorganisms 08 00287 g001 550
Figure 1. Study population flowchart. CNS: Central Nervous System.
Figure 1. Study population flowchart. CNS: Central Nervous System.
Microorganisms 08 00287 g001
Microorganisms 08 00287 g002 550
Figure 2. Kaplan-Meier survival curve for total mortality of AB septic shock patients.
Figure 2. Kaplan-Meier survival curve for total mortality of AB septic shock patients.
Microorganisms 08 00287 g002
Table
Table 1. Characteristics of the total cohort of ICU patients with XDR-AB infection (total study population n = 44).
Table 1. Characteristics of the total cohort of ICU patients with XDR-AB infection (total study population n = 44).
Patients’ Characteristics
Age, years (mean ± SD)65.3 ± 17.6
Sex-males (n, %)29 (65.9%)
APACHE II score (median, min–max)24 (13–40)
Diabetes mellitus (n, %)10 (22.7%)
Chronic heart failure (n, %)5 (11.3%)
Cardiovascular disease (n, %)8 (18.2%)
Chronic kidney disease (n, %)9 (20.5%)
Cirrhosis (n, %)3 (6.8%)
Chronic corticosteroid intake (n, %)7 (15.9%)
Cancer under therapy (n, %)3 (6.8%)
Immuno-suppressive therapy (n, %)4 (9.1%)
Cause of admission (n, %)
Shock of any kind (n, %)10 (22.7%)
ARF, need for MV (n, %)16 (36.4%)
Coma (n, %)17 (38.6%)
Need for monitoring (n, %)1 (2.3%)
ARF: acute respiratory failure, MV: mechanical ventilation.
Table
Table 2. Characteristics of AB infection.
Table 2. Characteristics of AB infection.
Parameter
Blood culture positive (n, %)31 (70.5%)
Fulfil criteria for sepsis (n, %)39 (88.6%)
SOFA score 24 before (median, min–max)8 (3–18)
SOFA score on day 0 (median, min–max)11 (6–20)
Difference in SOFA (median, min–max)3 (2–10)
Fulfil criteria for septic shock (n, %)29 (65.9%)
Days from admission to sepsis, days (median, mix-max)21 (0–82)
Days from ICU admission to sepsis, days (median, min-max)12 (0–55)
Days from admission to positive blood cultures, days (median, mix-max)20 (3–82)
Days from admission to shock, days (median, mix-max)22 (0–82)
Duration of shock, days (median, mix-max)4 (1–14)
Lowest pH on day 0 (median, min-max)7.14 (6.8–7.44)
Levels of lactate on day 0 (median, min-max)3.4 (1–14)
PaO2/FiO2 ratio on day 0, (median, min-max)213 (41–463)
WBC count on day 0 (median, min-max)15,210 (900–46,100)
CRP on day 0 (median, min-max)185 (11–479)
Implications
ARDS (n, %)15 (34.1%)
AKI (n, %)27 (61.4%)
CRRT (n, %14 (31.8%)
Septic myopathy (n, %)3 (6.8%)
DIC/thrombopenia (n, %)24 (54.5%)
Liver dysfunction (n, %)18 (40.1%)
SOFA score: Sequential Organ Failure Assessment score; ICU: Intensive Care Unit; PaO2/FiO2 ratio: ratio of arterial oxygen partial pressure to fractional inspired oxygen; WBC: White Blood Cell; CRP: C-Reactive Protein; ARDS: Acute Respiratory Distress Syndrome; AKI: Acute Kidney Injury; CRRT: Continuous Renal Replacement Therapy; DIC: Disseminated Intravascular Coagulation.
Table
Table 3. Comparison between patients with colistin-resistant and colistin-sensitive A. baumannii bloodstream infection.
Table 3. Comparison between patients with colistin-resistant and colistin-sensitive A. baumannii bloodstream infection.
ParameterPatients with Colistin-Resistant A. baumannii Blood Stream Infection
n = 13		Patients with Colistin-Susceptible A. baumannii Blood Stream Infection
n = 18		p
Age, years (mean ± SD)67.6 ± 11.556.4 ± 19.90.07
APACHE II score (median, min–max)28 (14–40)18 (13–40)0.01
Charlson Comorbidity Index score (median, min–max)5 (3–10)4 (0–8)0.044
Diabetes mellitus (n, %)2 (15.4%)3 (16.7%)NS
Chronic heart failure (n, %)2 (15.4%)3 (16.7%)NS
Chronic kidney disease (n, %)2 (15.4%)3 (16.7%)NS
SOFA score 24 h before admission (median, min–max)9 (3–18)6 (3–12)NS
SOFA score on day 0 (median, min–max)14 (6–20)11 (7–16)NS
Difference in SOFA (median, min–max)2.5 (1–10)4 (4–4)NS
Fulfil criteria for septic shock (n, %)13 (100%)14 (77.8%)NS
Days from admission to sepsis, days (median, mix–max)14.5 (0–82)14 (3–38)NS
Days from ICU admission to sepsis, days (median, min–max)10 (0–55)10.5 (0–31)NS
Days from admission to positive blood cultures, days (median, mix–max)14 (5–82)15 (3–50)NS
Days from admission to shock, days (median, mix–max)18 (0–82)12.5 (0–20)NS
Duration of shock, days (median, mix–max)1 (0.5–14)5.5 (1–14)0.04
Days from sepsis to death (median, mix–max)3 (1–25)4 (2–14)0.006
Lowest pH on day 0 (median, min–max)7.03 (6.9–7.33)7.27 (7.11–7.44)0.03
Levels of lactate on day 0 (median, min–max)2.8 (1–14)1.5 (0.8–8.8)0.0001
Maximum levels of lactate during hospitalization (median, min–max)11.4 (1.4–26)1.7 (1–9.1)0.0001
PaO2/FiO2 ratio on day 0, (median, min–max)198 (41–463)212 (105–385)NS
WBC count on day 0 (median, min–max)14,500 (900–28,200)14,500 (2,700–46,100)NS
CRP on day 0 (median, min–max)86.5 (11–430)172 (28–451)NS
Implications
ARDS (n, %)5 (38.5%)4 (22.2%)NS
AKI (n, %)12 (92.3%)6 (33.3%)NS
Septic myopathy (n, %)02 (11.1)-
DIC/thrombopenia (n, %)12 (92.3%)3 (16.7%)0.02
Liver dysfunction (n, %)8 (61.5%)5 (27.8%)NS
Mortality, overall13 (100%)7 (50%)0.001
Sepsis-related mortality9 (69.2%)4 (22.2%)0.003
SOFA score: Sequential Organ Failure Assessment score; PaO2/FiO2 ratio: ratio of arterial oxygen partial pressure to fractional inspired oxygen; WBC: White Blood Cell; CRP: C-Reactive Protein; ARDS: Acute Respiratory Distress Syndrome; AKI: Acute Kidney Injury; DIC: Disseminated Intravascular Coagulation; NS: not significant.

Share and Cite

MDPI and ACS Style

Papathanakos, G.; Andrianopoulos, I.; Papathanasiou, A.; Priavali, E.; Koulenti, D.; Koulouras, V. Colistin-Resistant Acinetobacter Baumannii Bacteremia: A Serious Threat for Critically Ill Patients. Microorganisms 2020, 8, 287. https://doi.org/10.3390/microorganisms8020287

AMA Style

Papathanakos G, Andrianopoulos I, Papathanasiou A, Priavali E, Koulenti D, Koulouras V. Colistin-Resistant Acinetobacter Baumannii Bacteremia: A Serious Threat for Critically Ill Patients. Microorganisms. 2020; 8(2):287. https://doi.org/10.3390/microorganisms8020287

Chicago/Turabian Style

Papathanakos, Georgios, Ioannis Andrianopoulos, Athanasios Papathanasiou, Efthalia Priavali, Despoina Koulenti, and Vasilios Koulouras. 2020. "Colistin-Resistant Acinetobacter Baumannii Bacteremia: A Serious Threat for Critically Ill Patients" Microorganisms 8, no. 2: 287. https://doi.org/10.3390/microorganisms8020287

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop