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Article

Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini

1
Division of Genetics and Molecular Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
2
Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
3
Central European Institute of Technology, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(2), 204; https://doi.org/10.3390/microorganisms8020204
Received: 29 November 2019 / Revised: 23 January 2020 / Accepted: 30 January 2020 / Published: 1 February 2020
(This article belongs to the Special Issue Biology and Pathogenesis of Staphylococcus Infection)
Members of the genus Staphylococcus are widespread in nature and occupy a variety of niches, however, staphylococcal colonization of animals in the Antarctic environment has not been adequately studied. Here, we describe the first isolation and characterization of two Staphylococcus intermedius group (SIG) members, Staphylococcus delphini and Staphylococcus pseudintermedius, in Antarctic wildlife. Staphylococcus delphini were found exclusively in Adélie penguins. The report of S. pseudintermedius from Weddell seals confirmed its occurrence in all families of the suborder Caniformia. Partial RNA polymerase beta-subunit (rpoB) gene sequencing, repetitive PCR fingerprinting with the (GTG)5 primer, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry gave consistent identification results and proved to be suitable for identifying SIG members. Comparative genomics of S. delphini isolates revealed variable genomic elements, including new prophages, a novel phage-inducible chromosomal island, and numerous putative virulence factors. Surface and extracellular protein distribution were compared between genomes and showed strain-specific profiles. The pathogenic potential of S. delphini was enhanced by a novel type of exfoliative toxin, trypsin-like serine protease cluster, and enterotoxin C. Detailed analysis of phenotypic characteristics performed on six Antarctic isolates of S. delphini and eight reference strains from different animal sources enabled us to emend the species description of S. delphini. View Full-Text
Keywords: Staphylococcus delphini; Staphylococcus pseudintermedius; Antarctica; mobile genetic elements; surface proteins; exfoliative toxin; Adélie penguin; Weddell seal Staphylococcus delphini; Staphylococcus pseudintermedius; Antarctica; mobile genetic elements; surface proteins; exfoliative toxin; Adélie penguin; Weddell seal
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MDPI and ACS Style

Vrbovská, V.; Sedláček, I.; Zeman, M.; Švec, P.; Kovařovic, V.; Šedo, O.; Laichmanová, M.; Doškař, J.; Pantůček, R. Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini. Microorganisms 2020, 8, 204. https://doi.org/10.3390/microorganisms8020204

AMA Style

Vrbovská V, Sedláček I, Zeman M, Švec P, Kovařovic V, Šedo O, Laichmanová M, Doškař J, Pantůček R. Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini. Microorganisms. 2020; 8(2):204. https://doi.org/10.3390/microorganisms8020204

Chicago/Turabian Style

Vrbovská, Veronika, Ivo Sedláček, Michal Zeman, Pavel Švec, Vojtěch Kovařovic, Ondrej Šedo, Monika Laichmanová, Jiří Doškař, and Roman Pantůček. 2020. "Characterization of Staphylococcus intermedius Group Isolates Associated with Animals from Antarctica and Emended Description of Staphylococcus delphini" Microorganisms 8, no. 2: 204. https://doi.org/10.3390/microorganisms8020204

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