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Article

Coupling an Electroactive Pseudomonas putida KT2440 with Bioelectrochemical Rhamnolipid Production

1
Institute of Applied Microbiology—iAMB, Aachen Biology and Biotechnology—ABBt, RWTH Aachen University, 52074 Aachen, Germany
2
Leibniz Institute for Natural Product Research and Infection Biology—Hans Knöll Institute, 07745 Jena, Germany
3
Department of Environmental Microbiology, Helmholtz Centre for Environmental Research—UFZ, 04318 Leipzig, Germany
4
Faculty of Biological Sciences, Friedrich Schiller University Jena, 07745 Jena, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Microorganisms 2020, 8(12), 1959; https://doi.org/10.3390/microorganisms8121959
Received: 20 November 2020 / Revised: 7 December 2020 / Accepted: 8 December 2020 / Published: 10 December 2020
Sufficient supply of oxygen is a major bottleneck in industrial biotechnological synthesis. One example is the heterologous production of rhamnolipids using Pseudomonas putida KT2440. Typically, the synthesis is accompanied by strong foam formation in the reactor vessel hampering the process. It is caused by the extensive bubbling needed to sustain the high respirative oxygen demand in the presence of the produced surfactants. One way to reduce the oxygen requirement is to enable the cells to use the anode of a bioelectrochemical system (BES) as an alternative sink for their metabolically derived electrons. We here used a P. putida KT2440 strain that interacts with the anode using mediated extracellular electron transfer via intrinsically produced phenazines, to perform heterologous rhamnolipid production under oxygen limitation. The strain P. putida RL-PCA successfully produced 30.4 ± 4.7 mg/L mono-rhamnolipids together with 11.2 ± 0.8 mg/L of phenazine-1-carboxylic acid (PCA) in 500-mL benchtop BES reactors and 30.5 ± 0.5 mg/L rhamnolipids accompanied by 25.7 ± 8.0 mg/L PCA in electrode containing standard 1-L bioreactors. Hence, this study marks a first proof of concept to produce glycolipid surfactants in oxygen-limited BES with an industrially relevant strain. View Full-Text
Keywords: Pseudomonas putida; rhamnolipid; bioelectrochemical system; phenazines; redox mediator; microbial electrosynthesis; electrobiotechnology; metabolic engineering Pseudomonas putida; rhamnolipid; bioelectrochemical system; phenazines; redox mediator; microbial electrosynthesis; electrobiotechnology; metabolic engineering
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MDPI and ACS Style

Askitosari, T.D.; Berger, C.; Tiso, T.; Harnisch, F.; Blank, L.M.; Rosenbaum, M.A. Coupling an Electroactive Pseudomonas putida KT2440 with Bioelectrochemical Rhamnolipid Production. Microorganisms 2020, 8, 1959. https://doi.org/10.3390/microorganisms8121959

AMA Style

Askitosari TD, Berger C, Tiso T, Harnisch F, Blank LM, Rosenbaum MA. Coupling an Electroactive Pseudomonas putida KT2440 with Bioelectrochemical Rhamnolipid Production. Microorganisms. 2020; 8(12):1959. https://doi.org/10.3390/microorganisms8121959

Chicago/Turabian Style

Askitosari, Theresia D., Carola Berger, Till Tiso, Falk Harnisch, Lars M. Blank, and Miriam A. Rosenbaum 2020. "Coupling an Electroactive Pseudomonas putida KT2440 with Bioelectrochemical Rhamnolipid Production" Microorganisms 8, no. 12: 1959. https://doi.org/10.3390/microorganisms8121959

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