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Open AccessArticle

Encapsulated Bdellovibrio Powder as a Potential Bio-Disinfectant against Whiteleg Shrimp-Pathogenic Vibrios

by Haipeng Cao 1,*,†, Huicong Wang 2,†, Jingjing Yu 1, Jian An 3 and Jun Chen 2
1
National Pathogen Collection Center for Aquatic Animals, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China
2
Department of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, Jiangsu, China
3
Lianyungang Marine and Fisheries Development Promotion Center, Lianyungang 222000, Jiangsu, China
*
Author to whom correspondence should be addressed.
The first two authors contributed equally to this work.
Microorganisms 2019, 7(8), 244; https://doi.org/10.3390/microorganisms7080244
Received: 12 May 2019 / Revised: 2 August 2019 / Accepted: 4 August 2019 / Published: 7 August 2019
(This article belongs to the Special Issue Recent Advances in Applied Microbiology)
Liquid preparations of bdellovibrios are currently commercialized as water quality improvers to control bacterial pathogens in whiteleg shrimp Penaeus vannamei. However, the efficacy of these liquid preparations is significantly impaired due to a dramatic loss of viable cells during long-term room temperature storage. Thus, new formulations of bdellovibrios are greatly needed for high-stablility room-temperature storage. In the present study, the encapsulated powder of Bdellovibrio sp. strain F16 was prepared using spray drying with 20 g L−1 gelatin as the coating material under a spray flow of 750 L h−1, a feed rate of 12 mL min−1, and an air inlet temperature of 140 °C. It was found to have a cell density of 5.4 × 107 PFU g−1 and to have spherical microparticles with a wrinkled surface and a diameter of 3 μm to 12 μm. In addition, the encapsulated Bdellovibrio powder presented good storage stability with its cell density still remaining at 3.5 × 107 PFU g−1 after 120 days of room-temperature storage; it was safe for freshwater-farmed whiteleg shrimp with an LD50 over 1200 mg L−1, and it exhibited significant antibacterial and protective effects at 0.8 mg L−1 against shrimp-pathogenic vibrios. To our knowledge, this is the first report on a promising Bdellovibrio powder against shrimp vibrios with high stable room-temperature storage. View Full-Text
Keywords: Bdellovibrio powder; characterization; Vibrio; Penaeus vannamei Bdellovibrio powder; characterization; Vibrio; Penaeus vannamei
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Cao, H.; Wang, H.; Yu, J.; An, J.; Chen, J. Encapsulated Bdellovibrio Powder as a Potential Bio-Disinfectant against Whiteleg Shrimp-Pathogenic Vibrios. Microorganisms 2019, 7, 244.

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