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Article

Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite

1
Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, China
2
College of Life Sciences, Sichuan Normal University, Chengdu 610101, China
*
Authors to whom correspondence should be addressed.
Microorganisms 2019, 7(8), 225; https://doi.org/10.3390/microorganisms7080225
Received: 17 June 2019 / Revised: 18 July 2019 / Accepted: 29 July 2019 / Published: 30 July 2019
(This article belongs to the Special Issue Recent Advances in Applied Microbiology)
Oxidative stress response protects organisms from deleterious effects of reactive oxygen species (ROS), which can damage cellular components and cause disturbance of the cellular homeostasis. Although the defensive biochemical mechanisms have been extensively studied in yeast and other filamentous fungi, little information is available about Aspergillus oryzae. We investigated the effect of two oxidant agents (menadione sodium bisulfite, MSB, and hydrogen peroxide, H2O2) on cellular growth and antioxidant enzyme induction in A. oryzae. Results indicated severe inhibition of biomass and conidia production when high concentration of oxidants was used. Transcriptomic analysis showed an up-regulated expression of genes involved in oxidoreduction, such as catalase, glutathione peroxidase, and superoxide dismutase. In addition, it was observed that oxidative stress stimuli enhanced the expression of Yap1 and Skn7 transcription factors. Further, metabolomic analysis showed that glutathione content was increased in the oxidative treatments when compared with the control. Moreover, the content of unsaturated fatty acid decreased with oxidative treatment accompanying with the down-regulated expression of genes involved in linoleic acid biosynthesis. This study provided a global transcriptome characterization of oxidative stress response in A. oryzae, and can offer multiple target genes for oxidative tolerance improvement via genetic engineering. View Full-Text
Keywords: Aspergillus oryzae; oxidative stress; antioxidant enzymes; glutathione; fatty acids Aspergillus oryzae; oxidative stress; antioxidant enzymes; glutathione; fatty acids
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MDPI and ACS Style

Shao, H.; Tu, Y.; Wang, Y.; Jiang, C.; Ma, L.; Hu, Z.; Wang, J.; Zeng, B.; He, B. Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite. Microorganisms 2019, 7, 225. https://doi.org/10.3390/microorganisms7080225

AMA Style

Shao H, Tu Y, Wang Y, Jiang C, Ma L, Hu Z, Wang J, Zeng B, He B. Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite. Microorganisms. 2019; 7(8):225. https://doi.org/10.3390/microorganisms7080225

Chicago/Turabian Style

Shao, Huanhuan, Yayi Tu, Yijing Wang, Chunmiao Jiang, Long Ma, Zhihong Hu, Jiangfan Wang, Bin Zeng, and Bin He. 2019. "Oxidative Stress Response of Aspergillus oryzae Induced by Hydrogen Peroxide and Menadione Sodium Bisulfite" Microorganisms 7, no. 8: 225. https://doi.org/10.3390/microorganisms7080225

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