Next Article in Journal
Uncovering Bifidobacteria via Targeted Sequencing of the Mammalian Gut Microbiota
Previous Article in Journal
Replenishment of Hepatitis B Virus cccDNA Pool Is Restricted by Baseline Expression of Host Restriction Factors In Vitro
Open AccessArticle

Determining the Virulence Properties of Escherichia coli ST131 Containing Bacteriocin-Encoding Plasmids Using Short- and Long-Read Sequencing and Comparing Them with Those of Other E. coli Lineages

1
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20550-170, Brazil
2
Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713GZ Groningen, The Netherlands
3
Department of biology and Biological Engineering, Chalmers University of Technology, Chalmersplatsen 4, 412 96 Göteborg, Sweden
4
Departamento de Doenças Infecciosas e Parasitárias, Universidade Federal do Estado do Rio de Janeiro, Rua Voluntário da Patria, 21, Rio de Janeiro 941-901107, Brazil
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(11), 534; https://doi.org/10.3390/microorganisms7110534
Received: 19 September 2019 / Revised: 29 October 2019 / Accepted: 1 November 2019 / Published: 6 November 2019
(This article belongs to the Section Molecular Microbiology)
Escherichia coli ST131 is a clinical challenge due to its multidrug resistant profile and successful global spread. They are often associated with complicated infections, particularly urinary tract infections (UTIs). Bacteriocins play an important role to outcompete other microorganisms present in the human gut. Here, we characterized bacteriocin-encoding plasmids found in ST131 isolates of patients suffering from a UTI using both short- and long-read sequencing. Colicins Ia, Ib and E1, and microcin V, were identified among plasmids that also contained resistance and virulence genes. To investigate if the potential transmission range of the colicin E1 plasmid is influenced by the presence of a resistance gene, we constructed a strain containing a plasmid which had both the colicin E1 and blaCMY-2 genes. No difference in transmission range was found between transformant and wild-type strains. However, a statistically significantly difference was found in adhesion and invasion ability. Bacteriocin-producing isolates from both ST131 and non-ST131 lineages were able to inhibit the growth of other E. coli isolates, including other ST131. In summary, plasmids harboring bacteriocins give additional advantages for highly virulent and resistant ST131 isolates, improving the ability of these isolates to compete with other microbiota for a niche and thereby increasing the risk of infection.
Keywords: ST131; E. coli; bacteriocins; plasmids; AmpC-beta-lactamase; UTIs; virulence ST131; E. coli; bacteriocins; plasmids; AmpC-beta-lactamase; UTIs; virulence
MDPI and ACS Style

da Cruz Campos, A.C.; Cavallo, F.M.; L. Andrade, N.; van Dijl, J.M.; Couto, N.; Zrimec, J.; Lo Ten Foe, J.R.; P. Rosa, A.C.; V. Damasco, P.; Friedrich, A.W.; Chlebowicz-Flissikowska, M.A.; Rossen, J.W.A. Determining the Virulence Properties of Escherichia coli ST131 Containing Bacteriocin-Encoding Plasmids Using Short- and Long-Read Sequencing and Comparing Them with Those of Other E. coli Lineages. Microorganisms 2019, 7, 534.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop