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Open AccessArticle

Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.

by Jiaqi You 1,2,3, Kang Zhou 1,2, Xiaolin Liu 1,2, Mingde Wu 1,2,*, Long Yang 1,2, Jing Zhang 1,2, Weidong Chen 4 and Guoqing Li 1,2
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
U.S. Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA 99164, USA
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(11), 507;
Received: 8 September 2019 / Revised: 20 October 2019 / Accepted: 25 October 2019 / Published: 29 October 2019
(This article belongs to the Special Issue Viral Ecology in Terrestrial Ecosystems)
Trichoderma species are a group of fungi which is widely distributed in major terrestrial ecosystems; they are also commonly used as biocontrol agents for many plant diseases. A virus, namely Trichoderma harzianum hypovirus 1 (ThHV1), was identified in T. harzianum isolate T-70, and also infected isolate T-70D, together with its defective RNA (ThHV1-S). The ThHV1 genome possessed two Open Reading Frames (ORFs), namely ORF1 and ORF2. The start codon of ORF2 overlapped with the stop codon of ORF1 in a 43 nt long region. The polypeptide encoded by ORF2 of ThHV1 shared sequence similarities with those of betahypoviruses, indicating that ThHV1 is a novel member of Hypoviridea. Isolate T-70D, carrying both ThHV1 and ThHV1-S, showed abnormal biological properties, notably a decreased mycoparasitism ability when compared with isolate T-70. Both ThHV1 and ThHV1-S could be vertically transmitted to conidia and horizontally transmitted to T. harzianum isolate T-68 and T. koningiopsis T-51. The derivative strains carrying both ThHV1 and ThHV1-S showed decreased mycoparasitism ability, whereas strains carrying ThHV1 alone were normal, indicating that ThHV1-S is closely associated with the decreased mycoparasitism ability of T. harzianum isolate T-70D. ThHV1 was widely detected in isolates of T. harzianum, T. koningiopsis and T. atroviride originating from soil of China. Therefore, viruses in fungal biocontrol agents may also be a factor associated with the stability of their application. View Full-Text
Keywords: biological control; Trichoderma spp.; hypovirus; Botrytis cinerea biological control; Trichoderma spp.; hypovirus; Botrytis cinerea
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You, J.; Zhou, K.; Liu, X.; Wu, M.; Yang, L.; Zhang, J.; Chen, W.; Li, G. Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.. Microorganisms 2019, 7, 507.

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