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Open AccessArticle

Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.

by Jiaqi You 1,2,3, Kang Zhou 1,2, Xiaolin Liu 1,2, Mingde Wu 1,2,*, Long Yang 1,2, Jing Zhang 1,2, Weidong Chen 4 and Guoqing Li 1,2
1
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
2
The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
3
Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
4
U.S. Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA 99164, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(11), 507; https://doi.org/10.3390/microorganisms7110507
Received: 8 September 2019 / Revised: 20 October 2019 / Accepted: 25 October 2019 / Published: 29 October 2019
(This article belongs to the Special Issue Viral Ecology in Terrestrial Ecosystems)
Trichoderma species are a group of fungi which is widely distributed in major terrestrial ecosystems; they are also commonly used as biocontrol agents for many plant diseases. A virus, namely Trichoderma harzianum hypovirus 1 (ThHV1), was identified in T. harzianum isolate T-70, and also infected isolate T-70D, together with its defective RNA (ThHV1-S). The ThHV1 genome possessed two Open Reading Frames (ORFs), namely ORF1 and ORF2. The start codon of ORF2 overlapped with the stop codon of ORF1 in a 43 nt long region. The polypeptide encoded by ORF2 of ThHV1 shared sequence similarities with those of betahypoviruses, indicating that ThHV1 is a novel member of Hypoviridea. Isolate T-70D, carrying both ThHV1 and ThHV1-S, showed abnormal biological properties, notably a decreased mycoparasitism ability when compared with isolate T-70. Both ThHV1 and ThHV1-S could be vertically transmitted to conidia and horizontally transmitted to T. harzianum isolate T-68 and T. koningiopsis T-51. The derivative strains carrying both ThHV1 and ThHV1-S showed decreased mycoparasitism ability, whereas strains carrying ThHV1 alone were normal, indicating that ThHV1-S is closely associated with the decreased mycoparasitism ability of T. harzianum isolate T-70D. ThHV1 was widely detected in isolates of T. harzianum, T. koningiopsis and T. atroviride originating from soil of China. Therefore, viruses in fungal biocontrol agents may also be a factor associated with the stability of their application. View Full-Text
Keywords: biological control; Trichoderma spp.; hypovirus; Botrytis cinerea biological control; Trichoderma spp.; hypovirus; Botrytis cinerea
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You, J.; Zhou, K.; Liu, X.; Wu, M.; Yang, L.; Zhang, J.; Chen, W.; Li, G. Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.. Microorganisms 2019, 7, 507.

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