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The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots

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Laboratorio de Biorremediación, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Francisco Salazar 01145, Temuco, Chile
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Programa de Doctorado en Ciencias Mención Biología Celular y Molecular Aplicada, Universidad de La Frontera, Francisco Salazar 01145, Temuco, Chile
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Centro de Biotecnología Vegetal, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile
*
Author to whom correspondence should be addressed.
Microorganisms 2019, 7(11), 490; https://doi.org/10.3390/microorganisms7110490
Received: 12 September 2019 / Revised: 20 October 2019 / Accepted: 24 October 2019 / Published: 26 October 2019
(This article belongs to the Section Molecular Microbiology)
The endophytic strain Chaetomium cupreum isolated from metal-contaminated soil was inoculated in Eucalyptus globulus roots to identify genes involved in metal stress response and plant growth promotion. We analyzed the transcriptome of E. globulus roots inoculated with C. cupreum. De novo sequencing, assembly, and analysis were performed to identify molecular mechanisms involved in metal stress tolerance and plant growth promotion. A total of 393,371,743 paired-end reads were assembled into 135,155 putative transcripts. It was found that 663 genes significantly changed their expression in the presence of treatment, of which 369 were up-regulated and 294 were down-regulated. We found differentially expressed genes (DEGs) encoding metal transporters, transcription factors, stress and defense response proteins, as well as DEGs involved in auxin biosynthesis and metabolism. Our results showed that the inoculation of C. cupreum enhanced tolerance to metals and growth promotion on E. globulus. This study provides new information to understand molecular mechanisms involved in plant–microbe interactions under metals stress. View Full-Text
Keywords: Chaetomium cupreum; endophyte; Eucalyptus globulus roots; metal stress; transcriptome Chaetomium cupreum; endophyte; Eucalyptus globulus roots; metal stress; transcriptome
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Ortiz, J.; Soto, J.; Fuentes, A.; Herrera, H.; Meneses, C.; Arriagada, C. The Endophytic Fungus Chaetomium cupreum Regulates Expression of Genes Involved in the Tolerance to Metals and Plant Growth Promotion in Eucalyptus globulus Roots. Microorganisms 2019, 7, 490.

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