Next Article in Journal
Temporal Dynamics in Rumen Bacterial Community Composition of Finishing Steers during an Adaptation Period of Three Months
Next Article in Special Issue
Cyanobacterial Growth in Minimally Amended Anaerobic Digestion Effluent and Flue-Gas
Previous Article in Journal
Probiotic Bifunctionality of Bacillus subtilis—Rescuing Lactic Acid Bacteria from Desiccation and Antagonizing Pathogenic Staphylococcus aureus
Previous Article in Special Issue
Transcriptional Terminators Allow Leak-Free Chromosomal Integration of Genetic Constructs in Cyanobacteria
Review

Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology

1
Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK
2
Centre for Synthetic and Systems Biology, University of Edinburgh, Edinburgh EH9 3BF, UK
3
Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, UK
4
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Microorganisms 2019, 7(10), 409; https://doi.org/10.3390/microorganisms7100409
Received: 1 September 2019 / Revised: 22 September 2019 / Accepted: 24 September 2019 / Published: 29 September 2019
(This article belongs to the Special Issue The Emerging Role of Cyanobacteria in Green Biotechnology)
Recent advances in synthetic biology and an emerging algal biotechnology market have spurred a prolific increase in the availability of molecular tools for cyanobacterial research. Nevertheless, work to date has focused primarily on only a small subset of model species, which arguably limits fundamental discovery and applied research towards wider commercialisation. Here, we review the requirements for uptake of new strains, including several recently characterised fast-growing species and promising non-model species. Furthermore, we discuss the potential applications of new techniques available for transformation, genetic engineering and regulation, including an up-to-date appraisal of current Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein (CRISPR/Cas) and CRISPR interference (CRISPRi) research in cyanobacteria. We also provide an overview of several exciting molecular tools that could be ported to cyanobacteria for more advanced metabolic engineering approaches (e.g., genetic circuit design). Lastly, we introduce a forthcoming mutant library for the model species Synechocystis sp. PCC 6803 that promises to provide a further powerful resource for the cyanobacterial research community. View Full-Text
Keywords: CRISPR/Cas; CRISPRi; genetic circuits; genome engineering; inteins; genome-scale models; mutant library; optogenetics; serine integrase; sigma factors; synthetic biology CRISPR/Cas; CRISPRi; genetic circuits; genome engineering; inteins; genome-scale models; mutant library; optogenetics; serine integrase; sigma factors; synthetic biology
Show Figures

Figure 1

MDPI and ACS Style

Gale, G.A.R.; Schiavon Osorio, A.A.; Mills, L.A.; Wang, B.; Lea-Smith, D.J.; McCormick, A.J. Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology. Microorganisms 2019, 7, 409. https://doi.org/10.3390/microorganisms7100409

AMA Style

Gale GAR, Schiavon Osorio AA, Mills LA, Wang B, Lea-Smith DJ, McCormick AJ. Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology. Microorganisms. 2019; 7(10):409. https://doi.org/10.3390/microorganisms7100409

Chicago/Turabian Style

Gale, Grant A.R., Alejandra A. Schiavon Osorio, Lauren A. Mills, Baojun Wang, David J. Lea-Smith, and Alistair J. McCormick. 2019. "Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology" Microorganisms 7, no. 10: 409. https://doi.org/10.3390/microorganisms7100409

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop