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Microorganisms 2018, 6(4), 105; https://doi.org/10.3390/microorganisms6040105

Dynamics of a Perturbed Microbial Community during Thermophilic Anaerobic Digestion of Chemically Defined Soluble Organic Compounds

1
Department of Thematic Studies-Environmental Change, Linköping University, 581 83 Linköping, Sweden
2
Biogas Research Center, Linköping University, 581 83 Linköping, Sweden
3
Department of Molecular Science, Swedish University of Agricultural Science, Uppsala BioCenter, 75007 Uppsala, Sweden
*
Author to whom correspondence should be addressed.
Received: 10 September 2018 / Revised: 6 October 2018 / Accepted: 10 October 2018 / Published: 11 October 2018
(This article belongs to the Special Issue Metabolic Diversity of Anaerobic Microbial Communities)
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Abstract

Knowledge of microbial community dynamics in relation to process perturbations is fundamental to understand and deal with the instability of anaerobic digestion (AD) processes. This study aims to investigate the microbial community structure and function of a thermophilic AD process, fed with a chemically defined substrate, and its association with process performance stability. Next generation amplicon sequencing of 16S ribosomal RNA (rRNA) genes revealed that variations in relative abundances of the predominant bacterial species, Defluviitoga tunisiensis and Anaerobaculum hydrogeniformans, were not linked to the process performance stability, while dynamics of bacterial genera of low abundance, Coprothermobacter and Defluviitoga (other than D. tunisiensis), were associated with microbial community function and process stability. A decrease in the diversity of the archaeal community was observed in conjunction with process recovery and stable performance, implying that the high abundance of specific archaeal group(s) contributed to the stable AD. Dominance of hydrogenotrophic Methanoculleus particularly corresponded to an enhanced microbial acetate and propionate turnover capacity, whereas the prevalence of hydrogenotrophic Methanothermobacter and acetoclastic Methanosaeta was associated with instable AD. Acetate oxidation via syntrophic interactions between Coprothermobacter and Methanoculleus was potentially the main methane-formation pathway during the stable process. We observed that supplementation of Se and W to the medium improved the propionate turnover by the thermophilic consortium. The outcomes of our study provided insights into the community dynamics and trace element requirements in relation to the process performance stability of thermophilic AD. View Full-Text
Keywords: Thermophilic Anaerobic Digestion; process perturbation; process stability; microbial community dynamics; trace elements Thermophilic Anaerobic Digestion; process perturbation; process stability; microbial community dynamics; trace elements
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Šafarič, L.; Shakeri Yekta, S.; Liu, T.; Svensson, B.H.; Schnürer, A.; Bastviken, D.; Björn, A. Dynamics of a Perturbed Microbial Community during Thermophilic Anaerobic Digestion of Chemically Defined Soluble Organic Compounds. Microorganisms 2018, 6, 105.

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