Next Article in Journal
Defining Multidrug Resistance of Gram-Negative Bacteria in the Dutch–German Border Region—Impact of National Guidelines
Previous Article in Journal
Therapeutic Management of Pseudomonas aeruginosa Bloodstream Infection Non-Susceptible to Carbapenems but Susceptible to “Old” Cephalosporins and/or to Penicillins
Article Menu
Issue 1 (March) cover image

Export Article

Open AccessArticle
Microorganisms 2018, 6(1), 10; https://doi.org/10.3390/microorganisms6010010

Investigating Potential Chromosomal Rearrangements during Laboratory Culture of Neisseria gonorrhoeae

1
School of Life Sciences, Pharmacy, and Chemistry, Kingston University, Penrhyn Road, Kingston upon Thames KT1 2EE, UK
2
National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
*
Author to whom correspondence should be addressed.
Received: 1 December 2017 / Revised: 19 December 2017 / Accepted: 19 January 2018 / Published: 20 January 2018
Full-Text   |   PDF [1317 KB, uploaded 20 January 2018]   |  

Abstract

Comparisons of genome sequence data between different strains and isolates of Neisseria spp., such as Neisseria gonorrhoeae, reveal that over the evolutionary history of these organisms, large scale chromosomal rearrangements have occurred. Factors within the genomes, such as repetitive sequences and prophage, are believed to have contributed to these observations. However, the timescale in which rearrangements occur is not clear, nor whether it might be expected for them to happen in the laboratory. In this study, N. gonorrhoeae was repeatedly passaged in the laboratory and assessed for large scale chromosomal rearrangements. Using gonococcal strain NCCP11945, for which there is a complete genome sequence, cultures were passaged for eight weeks in the laboratory. The resulting genomic DNA was assessed using Pulsed Field Gel Electrophoresis, comparing the results to the predicted results from the genome sequence data. Three cultures generated Pulsed Field Gel Electrophoresis patterns that varied from the genomic data and were further investigated for potential chromosomal rearrangements. View Full-Text
Keywords: gonococcus; genome rearrangement; prophage; PFGE; pulsed field gel electrophoresis; genome sequence gonococcus; genome rearrangement; prophage; PFGE; pulsed field gel electrophoresis; genome sequence
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Spencer-Smith, R.; Gould, S.W.; Pulijala, M.; Snyder, L.A.S. Investigating Potential Chromosomal Rearrangements during Laboratory Culture of Neisseria gonorrhoeae. Microorganisms 2018, 6, 10.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Microorganisms EISSN 2076-2607 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top