Next Article in Journal
Methane Oxidation and Molecular Characterization of Methanotrophs from a Former Mercury Mine Impoundment
Next Article in Special Issue
Temporal Study of the Microbial Diversity of the North Arm of Great Salt Lake, Utah, U.S.
Previous Article in Journal
Tackling Drug Resistant Infection Outbreaks of Global Pandemic Escherichia coli ST131 Using Evolutionary and Epidemiological Genomics
Article Menu

Export Article

Open AccessArticle
Microorganisms 2015, 3(2), 268-289;

Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms

Kompetenzzentrum Holz GmbH, Altenbergerstraße 69, Linz 4040, Austria
Institute of Chemical Engineering, Research Area Biochemical Engineering, Vienna University of Technology, Gumpendorferstraße 1a, Vienna 1060, Austria
Department of Cell Biology, University of Salzburg, Hellbrunnerstr 34, Salzburg 5020, Austria
Author to whom correspondence should be addressed.
Academic Editors: Ricardo Amils and Elena González Toril
Received: 18 March 2015 / Revised: 28 April 2015 / Accepted: 20 May 2015 / Published: 8 June 2015
(This article belongs to the Special Issue Extremophiles)
PDF [2020 KB, uploaded 9 June 2015]


Halophilic microorganisms thrive at elevated concentrations of sodium chloride up to saturation and are capable of growing on a wide variety of carbon sources like various organic acids, hexose and also pentose sugars. Hence, the biotechnological application of these microorganisms can cover many aspects, such as the treatment of hypersaline waste streams of different origin. Due to the fact that the high osmotic pressure of hypersaline environments reduces the risk of contamination, the capacity for cost-effective non-sterile cultivation can make extreme halophilic microorganisms potentially valuable organisms for biotechnological applications. In this contribution, the stepwise use of screening approaches, employing design of experiment (DoE) on model media and subsequently using industrial waste as substrate have been implemented to investigate the applicability of halophiles to generate PHB from the industrial waste stream spent sulfite liquor (SSL). The production of PHB on model media as well as dilutions of industrial substrate in a complex medium has been screened for by fluorescence microscopy using Nile Blue staining. Screening was used to investigate the ability of halophilic microorganisms to withstand the inhibiting substances of the waste stream without negatively affecting PHB production. It could be shown that neither single inhibiting substances nor a mixture thereof inhibited growth in the investigated range, hence, leaving the question on the inhibiting mechanisms open. However, it could be demonstrated that some haloarchaea and halophilic bacteria are able to produce PHB when cultivated on 3.3% w/w dry matter spent sulfite liquor, whereas H. halophila was even able to thrive on 6.6% w/w dry matter spent sulfite liquor and still produce PHB. View Full-Text
Keywords: polyhydroxybutyrate; halophilic microorganisms; halophilic archaea; spent sulfite liquor polyhydroxybutyrate; halophilic microorganisms; halophilic archaea; spent sulfite liquor

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Weissgram, M.; Gstöttner, J.; Lorantfy, B.; Tenhaken, R.; Herwig, C.; Weber, H.K. Generation of PHB from Spent Sulfite Liquor Using Halophilic Microorganisms. Microorganisms 2015, 3, 268-289.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Microorganisms EISSN 2076-2607 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top