Next Article in Journal
High-Throughput Amplicon Sequencing Microbial Communities and Volatile Flavour Compounds of Naturally Air-Dried Yak Jerky from Two Regions of Xizang
Previous Article in Journal
Challenges and Adaptations in COVID-19 Seroprevalence Surveys: Insights from Kinshasa, the Democratic Republic of the Congo
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 14
Issue 9
10.3390/microorganisms14092057
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Isolation, Identification of Three Prometryn-Degrading Strains and Their Synthetic Consortium: Degradation Characteristics and Soil Remediation Potential

by
Dong Liang
1,†,
Wenru Wang
1,†,
Yuhao Wang
1,
Shuangcai Xiao
1,
Yu Hao
2,
Yutong Song
1,
Lirui Sun
1,
Qingmin Kong
1,* and
Jiaying Xin
1,*
1
College of Food Engineering, Harbin University of Commerce, Harbin 150028, China
2
Tourism & Cuisine College, Harbin University of Commerce, Harbin 150028, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2026, 14(9), 2057; https://doi.org/10.3390/microorganisms14092057
Submission received: 11 July 2026 / Revised: 28 August 2026 / Accepted: 7 September 2026 / Published: 15 September 2026
(This article belongs to the Section Environmental Microbiology)
Browse Figures
Review Reports Versions Notes

Abstract

Prometryn, a methylthio-s-triazine herbicide, persists widely in agricultural soils after long-term application, causing crop phytotoxicity and potential human health risks, and microbial degradation offers an eco-friendly, cost-effective strategy for remediation. In this study, three prometryn-degrading bacterial strains were isolated from long-term contaminated cornfield soil in Harbin, China, and identified as Pseudomonas sp. ZM-1, Achromobacter sp. ZM-2 and Stenotrophomonas sp. ZM-3 based on morphological, biochemical and 16S rDNA sequence analysis; strain ZM-3 is, to the best of our knowledge, the first reported pure-culture Stenotrophomonas isolate with confirmed prometryn-degrading capability. The three strains degraded 97.3%, 85.1% and 92.8% of 100 mg·L−1 prometryn within 48 h respectively. The 1:1:1 synthetic consortium removed 96.8% of prometryn in 15 h, showing markedly superior degradation efficiency compared with single strains, and its 12-h degradation rate reached 96.87% with a half-life of 2.2 h following response surface optimization. In soil microcosms spiked with 20 mg·kg−1 prometryn, the consortium reduced the pollutant half-life from 58.2 days to 7.8 days and achieved 96.3% removal after 30 days, alongside enhanced soil dehydrogenase, catalase and urease activities. High-throughput 16S rRNA gene sequencing revealed directional succession of the soil bacterial community, enrichment of core degrading taxa, and predicted enrichment of xenobiotic biodegradation pathways during remediation. Maize pot experiments confirmed that the consortium significantly alleviated prometryn phytotoxicity at the tested concentration, restoring plant growth parameters to 95–97% of the uncontaminated control. This study provides an efficient synthetic microbial consortium for bioremediation of prometryn-contaminated agricultural soils.
Keywords: prometryn; synthetic consortium; Stenotrophomonas; soil enzyme activity; maize detoxification; phytotoxicit prometryn; synthetic consortium; Stenotrophomonas; soil enzyme activity; maize detoxification; phytotoxicit
Graphical Abstract

Share and Cite

MDPI and ACS Style

Liang, D.; Wang, W.; Wang, Y.; Xiao, S.; Hao, Y.; Song, Y.; Sun, L.; Kong, Q.; Xin, J. Isolation, Identification of Three Prometryn-Degrading Strains and Their Synthetic Consortium: Degradation Characteristics and Soil Remediation Potential. Microorganisms 2026, 14, 2057. https://doi.org/10.3390/microorganisms14092057

AMA Style

Liang D, Wang W, Wang Y, Xiao S, Hao Y, Song Y, Sun L, Kong Q, Xin J. Isolation, Identification of Three Prometryn-Degrading Strains and Their Synthetic Consortium: Degradation Characteristics and Soil Remediation Potential. Microorganisms. 2026; 14(9):2057. https://doi.org/10.3390/microorganisms14092057

Chicago/Turabian Style

Liang, Dong, Wenru Wang, Yuhao Wang, Shuangcai Xiao, Yu Hao, Yutong Song, Lirui Sun, Qingmin Kong, and Jiaying Xin. 2026. "Isolation, Identification of Three Prometryn-Degrading Strains and Their Synthetic Consortium: Degradation Characteristics and Soil Remediation Potential" Microorganisms 14, no. 9: 2057. https://doi.org/10.3390/microorganisms14092057

APA Style

Liang, D., Wang, W., Wang, Y., Xiao, S., Hao, Y., Song, Y., Sun, L., Kong, Q., & Xin, J. (2026). Isolation, Identification of Three Prometryn-Degrading Strains and Their Synthetic Consortium: Degradation Characteristics and Soil Remediation Potential. Microorganisms, 14(9), 2057. https://doi.org/10.3390/microorganisms14092057

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop