Next Article in Journal
The Gut–Brain Axis in Post-Traumatic Stress Disorder: From Biological Mechanisms to Microbiome-Based Therapeutic Strategies—A Narrative Review
Previous Article in Journal
Effects of Different Cropping Patterns on Soil Microbial Community and Function in Ningxia Irrigation-Silted Soil
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 14
Issue 5
10.3390/microorganisms14051090
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Bifidobacterium animalis subsp. lactis 832 Alleviates DSS-Induced Colitis in a Murine Model by Regulating Gut Microbiota and Phospholipid Metabolism

by
Xintong Chen
1,2,3,†,
Qiushi Wang
1,2,†,
Xiaoya Guo
1,2,
Dan Li
1,2,
Xinyu Wu
1,2,
Xiaoya Li
1,2,
Xiaoyu Zheng
1,2,
Yangyang Li
1,2,
Shuangshuang Han
1,2,
Lu Feng
1,2,3,
Bin Liu
1,2,3,* and
Lei Wang
1,2,3,4,*
1
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China
2
Key Laboratory of Molecular Microbiology and Technology, Nankai University, Tianjin 300457, China
3
Nankai International Advanced Research Institute, Nankai University Shenzhen, Shenzhen 518045, China
4
Southwest United Graduate School, Kunming 650092, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2026, 14(5), 1090; https://doi.org/10.3390/microorganisms14051090
Submission received: 28 March 2026 / Revised: 5 May 2026 / Accepted: 5 May 2026 / Published: 11 May 2026
(This article belongs to the Section Gut Microbiota)
Versions Notes

Abstract

Inflammatory bowel disease (IBD) is a chronic intestinal disorder with recurrent inflammation for which effective therapeutic options remain limited. Probiotics from the Bifidobacterium genus have potential beneficial effects on the prevention of IBD by improving intestinal barrier integrity and modulating immune responses. However, whether these effects are mediated by the regulation of gut metabolism remains largely unclear. This study was designed to explore the protective effect of an infant-derived Bifidobacterium animalis subsp. lactis 832 (B. lactis 832) on dextran sulfate sodium (DSS)-induced colitis in mice and its underlying mechanism. B. lactis 832 treatment significantly alleviated colitis severity (p < 0.05), as evidenced by reduced weight loss, disease activity index (DAI), and colonic injury, accompanied by significantly decreased pro-inflammatory cytokine expression and increased Il10 expression (p < 0.05). It also improved intestinal barrier integrity and modulated gut microbiota composition by reducing potentially pathogenic bacteria while enriching beneficial taxa. Surprisingly, metabolomic analysis revealed that B. lactis 832 intervention enhanced intestinal phospholipid metabolism, particularly increasing phosphatidylethanolamine (PE) and phosphatidylcholine (PC) levels. Notably, PE or PC supplementation recapitulated the protective effects against DSS-induced colitis (p < 0.05). These findings suggest that B. lactis 832 alleviates colitis through microbiota-associated metabolic regulation, highlighting a key role for phospholipid metabolism in mediating probiotic effects.
Keywords: Bifidobacterium animalis; inflammatory bowel diseases; intestinal barrier integrity; gut microbiota; phosphatidylethanolamine; phosphatidylcholine Bifidobacterium animalis; inflammatory bowel diseases; intestinal barrier integrity; gut microbiota; phosphatidylethanolamine; phosphatidylcholine

Share and Cite

MDPI and ACS Style

Chen, X.; Wang, Q.; Guo, X.; Li, D.; Wu, X.; Li, X.; Zheng, X.; Li, Y.; Han, S.; Feng, L.; et al. Bifidobacterium animalis subsp. lactis 832 Alleviates DSS-Induced Colitis in a Murine Model by Regulating Gut Microbiota and Phospholipid Metabolism. Microorganisms 2026, 14, 1090. https://doi.org/10.3390/microorganisms14051090

AMA Style

Chen X, Wang Q, Guo X, Li D, Wu X, Li X, Zheng X, Li Y, Han S, Feng L, et al. Bifidobacterium animalis subsp. lactis 832 Alleviates DSS-Induced Colitis in a Murine Model by Regulating Gut Microbiota and Phospholipid Metabolism. Microorganisms. 2026; 14(5):1090. https://doi.org/10.3390/microorganisms14051090

Chicago/Turabian Style

Chen, Xintong, Qiushi Wang, Xiaoya Guo, Dan Li, Xinyu Wu, Xiaoya Li, Xiaoyu Zheng, Yangyang Li, Shuangshuang Han, Lu Feng, and et al. 2026. "Bifidobacterium animalis subsp. lactis 832 Alleviates DSS-Induced Colitis in a Murine Model by Regulating Gut Microbiota and Phospholipid Metabolism" Microorganisms 14, no. 5: 1090. https://doi.org/10.3390/microorganisms14051090

APA Style

Chen, X., Wang, Q., Guo, X., Li, D., Wu, X., Li, X., Zheng, X., Li, Y., Han, S., Feng, L., Liu, B., & Wang, L. (2026). Bifidobacterium animalis subsp. lactis 832 Alleviates DSS-Induced Colitis in a Murine Model by Regulating Gut Microbiota and Phospholipid Metabolism. Microorganisms, 14(5), 1090. https://doi.org/10.3390/microorganisms14051090

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop