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Correction to Microorganisms 2025, 13(12), 2684.
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Correction

Correction: Morales et al. Modification and Validation of a Reference Real-Time RT-PCR Method for the Detection of a New African Horse Sickness Virus Variant. Microorganisms 2025, 13, 2684

by
Jorge Morales
1,
María José Ruano
1,
Cristina Tena-Tomás
2,
Antoinette van Schalkwyk
3,
Eleni-Anna Loundras
4,
Marta Valero-Lorenzo
1,
Ana López-Herranz
1,
Marco Romito
3,
Carrie Batten
4,
Rubén Villalba
1 and
Montserrat Agüero
1,*
1
Laboratorio Central de Veterinaria, Ministry of Agriculture, Fisheries and Food, 28110 Algete, Spain
2
Tecnologías y Servicios Agrarios, S.A. (TRAGSATEC), 28037 Madrid, Spain
3
Agricultural Research Council, Onderstepoort Veterinary Institute, Onderstepoort 0110, South Africa
4
The Pirbright Institute, Woking GU24 0NF, UK
*
Author to whom correspondence should be addressed.
Microorganisms 2026, 14(4), 880; https://doi.org/10.3390/microorganisms14040880
Submission received: 27 February 2026 / Accepted: 2 March 2026 / Published: 14 April 2026
(This article belongs to the Special Issue Animal Viral Infectious Diseases)
Browse Figure
Versions Notes
In the original publication [1], there was a mistake in Figure 1 as published. The sequence of the forward primer has not changed; it is the same as the one in the original 2008 Agüero method and reads 5′-CCAGTAGGCCAGATCAACAG-3′. However, the first “C” was missing in Figure 1C. The corrected Figure 1 appears below.
The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.

Reference

  1. Morales, J.; Ruano, M.J.; Tena-Tomás, C.; van Schalkwyk, A.; Loundras, E.-A.; Valero-Lorenzo, M.; López-Herranz, A.; Romito, M.; Batten, C.; Villalba, R.; et al. Modification and Validation of a Reference Real-Time RT-PCR Method for the Detection of a New African Horse Sickness Virus Variant. Microorganisms 2025, 13, 2684. [Google Scholar] [CrossRef] [PubMed]
Microorganisms 14 00880 g001
Figure 1. Alignment of the primers/probe described by Agüero (2008), Guthrie (2013), and the modified-Agüero method with segment 7 of the new AHSV strain from the RSA. (A) Alignment of the forward (AHS-1F-A2008) and reverse (AHS-1R-A2008) primers and probe (AHS-1P-A2008) of the Agüero 2008 method with the target sequence of the AHSV strain from the RSA. (B) Alignment of the forward (AHS-F-G) and reverse (AHS-R-G) primers and probe (AHS-P-G) of the Guthrie 2013 method with the target sequence of the AHSV strain from the RSA. (C) Alignment of the forward (AHS-1F-A2024) and reverse (AHS-1R-A2024) primers and probe (AHS-P-A2024) of the modified-Agüero rRT-PCR method with the target sequence of the AHSV strain from the RSA. The sequences are shown as cDNA; the primers and probes are depicted by coloured arrows and located at the annealing site of the cDNA target strand. The sequence of the primers and probes is shown aligned with the opposite strand to facilitate the visualisation of mismatches. Mismatches are highlighted in yellow and modifications of the primer/probe are shown in green.
Figure 1. Alignment of the primers/probe described by Agüero (2008), Guthrie (2013), and the modified-Agüero method with segment 7 of the new AHSV strain from the RSA. (A) Alignment of the forward (AHS-1F-A2008) and reverse (AHS-1R-A2008) primers and probe (AHS-1P-A2008) of the Agüero 2008 method with the target sequence of the AHSV strain from the RSA. (B) Alignment of the forward (AHS-F-G) and reverse (AHS-R-G) primers and probe (AHS-P-G) of the Guthrie 2013 method with the target sequence of the AHSV strain from the RSA. (C) Alignment of the forward (AHS-1F-A2024) and reverse (AHS-1R-A2024) primers and probe (AHS-P-A2024) of the modified-Agüero rRT-PCR method with the target sequence of the AHSV strain from the RSA. The sequences are shown as cDNA; the primers and probes are depicted by coloured arrows and located at the annealing site of the cDNA target strand. The sequence of the primers and probes is shown aligned with the opposite strand to facilitate the visualisation of mismatches. Mismatches are highlighted in yellow and modifications of the primer/probe are shown in green.
Microorganisms 14 00880 g001
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MDPI and ACS Style

Morales, J.; Ruano, M.J.; Tena-Tomás, C.; van Schalkwyk, A.; Loundras, E.-A.; Valero-Lorenzo, M.; López-Herranz, A.; Romito, M.; Batten, C.; Villalba, R.; et al. Correction: Morales et al. Modification and Validation of a Reference Real-Time RT-PCR Method for the Detection of a New African Horse Sickness Virus Variant. Microorganisms 2025, 13, 2684. Microorganisms 2026, 14, 880. https://doi.org/10.3390/microorganisms14040880

AMA Style

Morales J, Ruano MJ, Tena-Tomás C, van Schalkwyk A, Loundras E-A, Valero-Lorenzo M, López-Herranz A, Romito M, Batten C, Villalba R, et al. Correction: Morales et al. Modification and Validation of a Reference Real-Time RT-PCR Method for the Detection of a New African Horse Sickness Virus Variant. Microorganisms 2025, 13, 2684. Microorganisms. 2026; 14(4):880. https://doi.org/10.3390/microorganisms14040880

Chicago/Turabian Style

Morales, Jorge, María José Ruano, Cristina Tena-Tomás, Antoinette van Schalkwyk, Eleni-Anna Loundras, Marta Valero-Lorenzo, Ana López-Herranz, Marco Romito, Carrie Batten, Rubén Villalba, and et al. 2026. "Correction: Morales et al. Modification and Validation of a Reference Real-Time RT-PCR Method for the Detection of a New African Horse Sickness Virus Variant. Microorganisms 2025, 13, 2684" Microorganisms 14, no. 4: 880. https://doi.org/10.3390/microorganisms14040880

APA Style

Morales, J., Ruano, M. J., Tena-Tomás, C., van Schalkwyk, A., Loundras, E.-A., Valero-Lorenzo, M., López-Herranz, A., Romito, M., Batten, C., Villalba, R., & Agüero, M. (2026). Correction: Morales et al. Modification and Validation of a Reference Real-Time RT-PCR Method for the Detection of a New African Horse Sickness Virus Variant. Microorganisms 2025, 13, 2684. Microorganisms, 14(4), 880. https://doi.org/10.3390/microorganisms14040880

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