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Microorganisms
Volume 13
Issue 12
10.3390/microorganisms13122738
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Complete Genome Sequence Analysis and Probiotic Characterisation of Lactiplantibacillus plantarum Y300 Isolated from Traditional Free-Range Chickens

by
Xiaoyu Zhang
1,
Xuehuai Shen
2,
Dongdong Yin
2,
Jieru Wang
2,
Ruihong Zhao
2,
Yin Dai
2,
Erhui Jin
1,*,
Xiaocheng Pan
2 and
Lei Yin
2,*
1
College of Animal Science, Anhui Science and Technology University, Chuzhou 233100, China
2
Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China
*
Authors to whom correspondence should be addressed.
Microorganisms 2025, 13(12), 2738; https://doi.org/10.3390/microorganisms13122738 (registering DOI)
Submission received: 28 October 2025 / Revised: 25 November 2025 / Accepted: 26 November 2025 / Published: 30 November 2025
(This article belongs to the Special Issue Probiotics, Prebiotics, and Gut Microbes—Second Edition)
Versions Notes

Abstract

As a core strategy for antibiotic replacement, probiotics have two advantages insofar as they enhance both animal productivity and pathogen suppression. In this study, we screened the intestines of antibiotic-naïve chickens for broad-spectrum antimicrobial lactic acid bacteria (LAB) with natural adaptability, based on the host–microbiota coevolution theory, and systematically evaluated their potential for development as poultry probiotics. We isolated a LAB strain, Lactiplantibacillus plantarum Y300, from traditional native free-range chickens, which showed strong inhibitory activity against avian pathogenic Salmonella, Escherichia coli, and Staphylococcus aureus. In vitro experiments indicated that the Lpb. plantarum strain Y300 had no hemolytic activity; excellent acid-producing ability;an outstanding tolerance to bile salts, low-pH environments, and simulated gastrointestinal fluids; a positive hydrophobic interaction with xylene, and good auto-aggregation characteristics. It also displayed a relatively high antioxidant capacity. Whole-genome sequencing revealed that the genome of Lpb. plantarum Y300 was approximately 3.05 mb, with a GC content of 44.74%. The main carbohydrate-active enzyme and bacteriocin genes were predicted in the Y300 genome, and no virulence genes or drug-resistance genes were detected. In summary, this study suggests that Lpb. plantarum Y300 has potential utility as a probiotic, and lays the theoretical foundation for the further development of microecological preparations of avian-sourced LAB.
Keywords: Lactiplantibacillus plantarum; safety; probiotic property; whole-genome sequencing; traditional free-range chickens Lactiplantibacillus plantarum; safety; probiotic property; whole-genome sequencing; traditional free-range chickens

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MDPI and ACS Style

Zhang, X.; Shen, X.; Yin, D.; Wang, J.; Zhao, R.; Dai, Y.; Jin, E.; Pan, X.; Yin, L. Complete Genome Sequence Analysis and Probiotic Characterisation of Lactiplantibacillus plantarum Y300 Isolated from Traditional Free-Range Chickens. Microorganisms 2025, 13, 2738. https://doi.org/10.3390/microorganisms13122738

AMA Style

Zhang X, Shen X, Yin D, Wang J, Zhao R, Dai Y, Jin E, Pan X, Yin L. Complete Genome Sequence Analysis and Probiotic Characterisation of Lactiplantibacillus plantarum Y300 Isolated from Traditional Free-Range Chickens. Microorganisms. 2025; 13(12):2738. https://doi.org/10.3390/microorganisms13122738

Chicago/Turabian Style

Zhang, Xiaoyu, Xuehuai Shen, Dongdong Yin, Jieru Wang, Ruihong Zhao, Yin Dai, Erhui Jin, Xiaocheng Pan, and Lei Yin. 2025. "Complete Genome Sequence Analysis and Probiotic Characterisation of Lactiplantibacillus plantarum Y300 Isolated from Traditional Free-Range Chickens" Microorganisms 13, no. 12: 2738. https://doi.org/10.3390/microorganisms13122738

APA Style

Zhang, X., Shen, X., Yin, D., Wang, J., Zhao, R., Dai, Y., Jin, E., Pan, X., & Yin, L. (2025). Complete Genome Sequence Analysis and Probiotic Characterisation of Lactiplantibacillus plantarum Y300 Isolated from Traditional Free-Range Chickens. Microorganisms, 13(12), 2738. https://doi.org/10.3390/microorganisms13122738

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