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Microorganisms
Volume 13
Issue 10
10.3390/microorganisms13102306
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Degradation of Dioxins and DBF in Urban Soil Microcosms from Lausanne (Switzerland): Functional Performance of Indigenous Bacterial Strains

by
Rita Di Martino
1,
Mylène Soudani
1,2,
Patrik Castiglioni
1,
Camille Rime
3,
Yannick Gillioz
4,
Loïc Sartori
1,
Tatiana Proust
1,
Flavio Neves Dos Santos
1,
Fiorella Lucarini
5,* and
Davide Staedler
1,4,*
1
TIBIO Suisse Romande, Chemin de Bérée 4C, 1010 Lausanne, Switzerland
2
Group for Functionalized Biomaterials, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland
3
TIBIOLab Sàrl, Route d’Yverdon 34, 1373 Chavornay, Switzerland
4
Department of Biomedical Sciences, University of Lausanne, Rue du Bugnon 27, 1011 Lausanne, Switzerland
5
School of Engineering and Architecture of Fribourg, Institute of Chemical Technology, HES-SO University of Applied Sciences and Arts of Western Switzerland, Boulevard de Pérolles 80, 1700 Fribourg, Switzerland
*
Authors to whom correspondence should be addressed.
Microorganisms 2025, 13(10), 2306; https://doi.org/10.3390/microorganisms13102306 (registering DOI)
Submission received: 5 September 2025 / Revised: 29 September 2025 / Accepted: 1 October 2025 / Published: 5 October 2025
(This article belongs to the Special Issue Interaction Between Microorganisms and Environment)
Review Reports Versions Notes

Abstract

Urban soils are often affected by long-term deposition of persistent organic pollutants, including polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs). This study evaluated the biodegradation potential of indigenous bacterial strains isolated from chronically contaminated soils in Lausanne, Switzerland. Using selective enrichment techniques, five strains were isolated, with no biosafety concerns for human health and environmental applications. These isolates were screened for their ability to degrade dibenzofuran (DBF) and 2,7-dichlorodibenzo-p-dioxin (2,7-DD) under mineral medium conditions. A simplified two-strain consortium (Acinetobacter bohemicus and Bacillus velezensis) and a broader five-strain co-culture were then applied to real soil microcosms over a 24-week period. This work provides the first experimental evidence that A. bohemicus and B. velezensis can degrade DBF and 2,7-DD under controlled conditions. Dioxin concentrations were monitored at 4, 8, and 24 weeks using a Gas Chromatography Mass Spectrometry (GC-MS). In laboratory conditions, co-cultures showed enhanced degradation compared to individual strains, likely due to metabolic complementarity. In soil, the simplified two-strain consortium performed better at dioxin degradation, especially at earlier time points. Although no statistically significant reductions were observed due to high variability and limited sample size, consistent trends emerged, particularly at the most contaminated site. These findings support the relevance of testing bioremediation strategies under realistic environmental conditions.
Keywords: dioxins; bioremediation; soil pollution; indigenous bacteria; dibenzofuran; co-culture; urban soil; 2,7-dichlorodibenzo-p-dioxin dioxins; bioremediation; soil pollution; indigenous bacteria; dibenzofuran; co-culture; urban soil; 2,7-dichlorodibenzo-p-dioxin

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MDPI and ACS Style

Di Martino, R.; Soudani, M.; Castiglioni, P.; Rime, C.; Gillioz, Y.; Sartori, L.; Proust, T.; Neves Dos Santos, F.; Lucarini, F.; Staedler, D. Degradation of Dioxins and DBF in Urban Soil Microcosms from Lausanne (Switzerland): Functional Performance of Indigenous Bacterial Strains. Microorganisms 2025, 13, 2306. https://doi.org/10.3390/microorganisms13102306

AMA Style

Di Martino R, Soudani M, Castiglioni P, Rime C, Gillioz Y, Sartori L, Proust T, Neves Dos Santos F, Lucarini F, Staedler D. Degradation of Dioxins and DBF in Urban Soil Microcosms from Lausanne (Switzerland): Functional Performance of Indigenous Bacterial Strains. Microorganisms. 2025; 13(10):2306. https://doi.org/10.3390/microorganisms13102306

Chicago/Turabian Style

Di Martino, Rita, Mylène Soudani, Patrik Castiglioni, Camille Rime, Yannick Gillioz, Loïc Sartori, Tatiana Proust, Flavio Neves Dos Santos, Fiorella Lucarini, and Davide Staedler. 2025. "Degradation of Dioxins and DBF in Urban Soil Microcosms from Lausanne (Switzerland): Functional Performance of Indigenous Bacterial Strains" Microorganisms 13, no. 10: 2306. https://doi.org/10.3390/microorganisms13102306

APA Style

Di Martino, R., Soudani, M., Castiglioni, P., Rime, C., Gillioz, Y., Sartori, L., Proust, T., Neves Dos Santos, F., Lucarini, F., & Staedler, D. (2025). Degradation of Dioxins and DBF in Urban Soil Microcosms from Lausanne (Switzerland): Functional Performance of Indigenous Bacterial Strains. Microorganisms, 13(10), 2306. https://doi.org/10.3390/microorganisms13102306

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