Production of a Rich Fertilizer Base for Plants from Waste Organic Residues by Microbial Formulation Technology

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

This study focuses on different methods of sustainably introducing nutrients from agriculture food waste into the soil by microbial formulation technology. The topic is interesting. However, there are several issues needed to be addressed.

1 Ln 105 - Ln 113, the relevant reference should be provided.  

2 Table 1,  it is weak to show the typical possible sources. The details and relevant references should be added in this table.

3 Ln 366, Microbial consortia part, important details and logic should be described. Please make the key points stronger than current version. You can add more findings and relevant references. 

4 Page 17, the table content is difficult to follow the information. You should modify the table style of this page. 

5 Future aspects, it should be significantly improved. It should include the scientific advances and limitations of the microbial formulation technology to date. Also, please provide more essential key points on future aspects. 

Comments on the Quality of English Language

Moderate english editing is required.

Author Response

We (Authors) thank you for your review and valuable suggestions & corrections pointed. We have agreed with your comments and each point raised were critically evaluated and modified accordingly. The corrections were highlighted with red color font in the Manuscript. Please find the attached file for the point by point response to your comments.

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

Dear authors,

This work is very interesting, well organized and well written, however there are some comments should be considered before publication such as: 

-          The abstract seems like addressing the aims of the review, therefore the abstract should be rewritten to include the most important points such as the aims, the approaches or methodology, the findings, and the contribution to the field

-          In figure two, the Eq. is not indicative because it is not known what does it refer to, as well to write Eq. you should write its reference

-          Please magnify the figure in order to show the bottom part clearly

-          In line 180, change mg year not mg/year

-          The conclusion should be extended to include the most important findings and the contribution to the field. As well, "The current review aims" is not suitable in the conclusion, it is more suitable in the abstract or introduction

-          Please update the references from 2020 - 2024

Author Response

We (Authors) thank you for your review and valuable suggestions & corrections pointed. We have agreed with your comments and each point raised were critically evaluated and modified accordingly. The corrections were highlighted with red color font in the Manuscript. Please find the attached file for the point by point response to your comments.

Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

The suggestions for

“Formulation of waste compounds into nutrient-rich source for plants by microbial formulation technology”

 

 

 Suggested title: “Production of a rich fertilizer base for plants from waste organic residues by microbial technology”

 

Line 13

Delete: “…soil…”

 

Lines 31,32,33,34

Delete: “The equilibrium of the soil and rhizosphere's microbiota could be significantly impacted, so nutrient rich secondary raw materials can, nevertheless, offer a valuable foundation for modern mineral fertilizers—if the new formulations avoid overloading the soil-plant nexus with potentially toxic substances”.

Replace with: “The equilibrium of the soil and rhizosphere's microbiota could be significantly affected, and nutrient rich secondary raw materials can offer an improved mineral nutrition, if the new formulations avoid overloading the soil-plant nexus with potentially toxic substances”.

 

Lines 45, 46

delete blank spaces in the text.

 

Line 111

Delete: “…up. And, …”

Replace with: “…up, and…”

 

Figure 2: not very understandable figure, caption of the figure to be completed.

 

Line 58

Delete: “enclosed in the matrix of secondary nutrient flows”

 

Line 173, 174

At least one bibliographic citation is required.

 

Line 180

Delete: “…mg year…”

Replace with: “…Mg * year ^-1  ” or “…Mg / year …”

 

Line 256

Delete: “.”

 

Line 334

Delete: “…B…”

Replace with: “…Bacillus…”

 

Line 356

Add: “…Arbuscular Mycorrhiza…” after (AM)

 

Line 416

Add: at the beginning “Biopriming is the seeds hydration utilizing a biological or a biocontrol ingredient.”

 

 

Line 418,419

Delete: “…The procedure of seed priming involves soaking the seeds in any solution that contains the necessary priming ingredient, then drying them again.…”

Replace with: “…the procedure of seed priming involves soaking the seeds in a suitable solution, then drying them again.…”

 

Line 421, 422

Delete: “..Biopriming is the phrase for hydration utilizing any biological ingredient, 421

or in this case, a biocontrol agent..”

 

Line 425

Delete: “…In…”

Replace with: “…in…”

 

Line 430

Add: (Plant Growth-Promoting Rizhobacteria) after PGPR

 

Line 438

Delete: “…and offer…”

Replace with: “…offering…”

 

Line 439, 440

Delete: “…stand establishing…”

 

Line 454

Delete: “…By…”

Replace with: “…by…”

 

Line 463

Delete: “…The immobilized…”

Replace with: “…the immobilized …”

 

Line 470

At the end of the sentence only a full stop

 

Line 494, 496

Delete: “…T.…”

Replace with: “…Trichoderma …”

 

Line 497

Delete: “…S.…”

Replace with: “…Sclerotium …”

 

Line 498

Delete: “…T.…F…”

Replace with: “…Trichoderma …Fusarium….”

 

Line 501

Delete: “…P.…P…”

Replace with: “…Pthium …Pseudomonas….”

 

Line 537

Delete: “…20 g/l…”

Replace with: “…20 g/L….”

 

Line 566

Add: “…(Oil Palm Empty Fruit Bunch)…” before “OPEFB”

 

Line 579

Delete: “…effective microorganism…”

Replace with: “…Effective Microorganisms….”

 

Line 621

Add: “may” before “decreases”

 

Line 624, 625, 626, 627

Delete: “…The current review aims to explain the effective introduction of microorganisms to the soil system that ensures its sufficient colonization, tailored to carry out specific functions and to gain insight into the mechanism and understanding of how soil microbes boost plant growth under conditions when nutrients are delivered in not available, waste-born form.…”

Replace with: “…The current review aims to explain the introduction of microorganisms effects to the soil system to ensures its sufficient colonization to carry out specific functions, and to improve understanding of how soil microbes boost plant growth under conditions when nutrients are delivered in not available, waste-born form.….”

 

 

Line 640

Add: “Structural variability” before “and Differentiation of Niches in the Rhizosphere…”

 

Line 751

Add: “Metabolic” before “load”

 

Line 761

Delete: “ Bacillus Firmus”

Replace with: “Bacillus firmus”

 

 

Author Response

We (Authors) thank you for your review and valuable suggestions & corrections pointed. We have agreed with your comments and each point raised were critically evaluated and modified accordingly. The corrections were highlighted with red color font in the Manuscript. Please find the attached file for the point by point response to your comments.

Author Response File: Author Response.pdf

Reviewer 4 Report

Comments and Suggestions for Authors

It is a review paper inform about formulation of waste compounds into nutrient-rich source for plants by microbial formulation technology. Utilization of waste into fertilizer as a part of closing the loop of nutrients carries a risk of biological imbalance of the soil microbiota.  The Authors present waste biomass as a source of nutrient material. The current review aims to explain the effective introduction of microorganisms to the soil system that ensures its sufficient colonization, tailored to carry out specific functions and to gain insight into the mechanism and understanding of how soil microbes boost plant growth under conditions when nutrients are delivered in not available, waste-born form. Therefore, the paper fits the journal scope. The overview of different forms of microbial formulations are interesting for the readership of the journal. 

Regarding the quality, the paper is rather well written although some weak points were found and below are my specific comments for the Authors. 

The Authors can give examples of foliar spray application. 

At the Figure 2. Model illustrating the direct & in direct mechanism of microbiota action in the soil system

can use word enzymes (phosphatases, nitrogenase, dehydrogenases etc.)

translate what is K and [X] in formula in page 4. 

At the Figure 3. Model illustrating nutrient liberation processes from not available to available forms to be 149

utilized by the plants.

-the letters are too small. The Authors should minimalist the figure of plant and give bigger the font the information of mobilization processes at the roots space.

Correct some editorial mistakes

page 5 line 190 small letter at the beginner of sentence 

page 13 line 359 problem with bracket.

Author Response

We (Authors) thank you for your review and valuable suggestions & corrections pointed. We have agreed with your comments and each point raised were critically evaluated and modified accordingly. The corrections were highlighted with red color font in the Manuscript. Please find the attached file for the point by point response to your comments.

Author Response File: Author Response.pdf