Antibacterial Activities of Ag/Cellulose Nanocomposites Derived from Marine Environment Algae against Bacterial Tooth Decay
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Chemicals
2.2. Algae Collection
2.3. Extracting Cellulose from U. lactuca Green Alga
2.4. Synthesis of Nanocellulose
2.5. Silver Nanoparticle Preparation
2.6. Biosynthesis of Ag/Cellulose Nanocomposites
2.7. Mixing of Fluoride with Ag/Cellulose Nanocomposites
2.8. Characterization of Ag/Cellulose Nanocomposites
2.8.1. FTIR Spectroscopy Analysis
2.8.2. X-ray Diffraction (XRD)
2.8.3. Energy-Dispersive Spectroscopy (EDS)
2.8.4. Scanning Electron Microscopy (SEM)
2.8.5. Transmission Electron Microscopy (TEM)
2.9. Antibacterial Activity of Ag/Cellulose Nanocomposites/Fluoride against Bacteria Isolated from Dental Caries
2.9.1. Ethical Approval
2.9.2. Collection and Isolation of Bacterial Strains
2.9.3. Identification of Bacterial Isolates
2.9.4. Antimicrobial Susceptibility Tests
2.9.5. Antibacterial Activities of Cellulose, AgNPs, Nanocellulose, and Ag/Cellulose Nanocomposites with and without Fluoride against Isolated Bacteria
3. Results and Discussion
3.1. Characterization of Ag/Cellulose Nanocomposites
FTIR Spectroscopy Analysis
3.2. X-ray Diffraction (XRD) of Ag/Cellulose Nanocomposites
3.3. Energy-Dispersive Spectroscopy (EDS) of Ag/Cellulose Nanocomposites
3.4. Scanning Electron Microscopy (SEM) of Ag/Cellulose Nanocomposites
3.5. Transmission Electron Microscopy (TEM) of Ag/Cellulose Nanocomposites
- Leuconosticmesenteroides
3.6. Isolation and Identification of Bacterial Isolates on Mitis Salivarius (M-S) Agar
Isolation and Identification of Bacterial Isolates on De Man, Rogosa and Sharpe (MRS) Media
3.7. Antibiotic Susceptibility Pattern for Different Isolates
3.8. Antibacterial Activity of Cellulose, Nanocellulose, AgNPs, and Ag/Cellulose Nanocomposites with and without Fluoride against Antibiotic-Resistant Bacterial Isolates
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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2 Theta | Sin Theta | hkl | D (nm) | Intensity % |
---|---|---|---|---|
11.5169 | 0.100334799 | 100 | 40.16 | 5.58 |
20.6775 | 0.17946759 | 111 | 33.08 | 10.51 |
23.2611 | 0.2016002 | 200 | 12.35 | 3.15 |
27.7198 | 0.239548602 | 211 | 26.12 | 45.55 |
28.9014 | 0.249546871 | 211 | 35.53 | 6.15 |
29.5627 | 0.25513104 | 211 | 65.04 | 0.95 |
30.9599 | 0.266901148 | 211 | 29.93 | 10.64 |
31.6265 | 0.272502759 | 220 | 38.83 | 32.05 |
32.1345 | 0.276765431 | 220 | 27.70 | 100 |
36.2324 | 0.310945169 | 310 | 42.63 | 2.42 |
37.2304 | 0.319210732 | 310 | 38.25 | 2.83 |
43.3728 | 0.369526204 | 321 | 20.84 | 1.3 |
45.4086 | 0.385975277 | 321 | 24.82 | 20.18 |
47.5831 | 0.403410353 | 400 | 15.39 | 1 |
49.0656 | 0.415214098 | 410 | 61.19 | 0.87 |
54.6861 | 0.4593171 | 421 | 21.08 | 14.53 |
55.8432 | 0.468262953 | 421 | 35.66 | 1.01 |
56.497 | 0.473296605 | 421 | 22.22 | 4.54 |
57.3153 | 0.479575026 | 422 | 23.61 | 13.84 |
58.2132 | 0.486436029 | 430 | 44.09 | 0.6 |
66.0993 | 0.545365585 | 521 | 37.59 | 1.66 |
67.3334 | 0.554360806 | 521 | 27.54 | 5.48 |
Test | Strepto thoraltensis | Strepto alactolyticus | Strepto mutans | Strepto salivarius | Strepto sanguinis | Staph lentus | Staph hominis | Granulicatella adiacens | Gemella sanguinis | Kytococcus | Kocuria kristinae | Aerococcus viridans | Leuconosticme senteroides |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
D-amygdalin | + | + | + | + | + | + | − | − | − | − | − | + | − |
Ala-Phe-Pro arylamidase | + | + | − | + | − | − | − | − | − | − | − | − | − |
Leucine arylamidase | + | + | − | + | + | − | − | + | − | − | − | − | − |
Alanine arylamidase | + | + | + | + | + | − | − | − | − | + | − | − | − |
D-ribose | − | − | − | − | − | − | − | − | − | − | − | − | − |
Novobiocin resistance | − | + | − | + | − | − | + | + | − | − | − | − | − |
Pullulan | + | + | − | − | − | + | − | − | − | − | − | − | − |
Bacitracin resistance | + | + | − | + | − | − | + | + | − | − | − | − | − |
Alpha-glucosidase | − | − | − | + | − | − | + | − | − | − | − | − | − |
D-galactose | + | + | + | + | + | + | − | − | − | − | + | + | − |
Beta-glucuronidase | − | − | − | − | − | − | − | − | − | − | − | − | − |
Phosphatase | − | − | − | − | − | − | + | − | − | − | − | − | − |
Arginine dihydrolase 2 | − | − | − | − | − | − | − | − | − | − | − | − | |
D-trehalose | + | + | + | − | + | − | + | − | + | − | − | − | − |
Alpha-mannosidase | − | − | − | − | − | − | − | − | − | − | − | − | − |
Saccharose/sucrose | + | + | + | + | + | + | + | + | − | − | + | + | + |
L-lactate alkalinization | − | − | − | − | − | − | + | − | − | − | − | − | − |
beta-glucuronidase | − | − | − | − | − | − | − | − | − | − | − | − | − |
N-acetyl-d-glucosamine | + | + | − | + | + | + | + | − | − | − | − | − | − |
Urease | − | − | − | + | + | − | + | − | − | − | − | − | − |
D-sorbitol | + | + | - | − | − | + | − | − | − | − | − | − | − |
Salicin | + | − | + | + | + | + | − | − | − | − | + | + | + |
arginine dihydrolase 1 | − | − | − | − | − | − | + | + | − | − | − | − | − |
D-xylose | + | + | − | − | − | + | − | − | − | − | − | + | − |
phosphatidylinositol phospholipase c | − | − | − | − | − | − | − | − | − | − | − | − | − |
Growth in 6.5% Nacl | − | − | − | − | − | − | + | − | − | − | − | − | − |
Novobiocin resistance | − | + | − | + | − | − | + | + | − | − | − | − | − |
Test | Lactobacillus acidophilus | Lactobacillus plantarum | Corynebacterium Group f-1 |
---|---|---|---|
Ala-Phe-Pro arylamidase | + | − | − |
Beta-galactosidase | + | − | − |
Alpha-mannosidase | − | − | − |
Beta-glucuronidase | − | − | − |
D-galactose | + | + | + |
L-lactate alkalinization | − | − | − |
D-Ribose | − | + | + |
D-sorbitol | − | + | − |
D-trehalose | + | + | − |
D-xylose | − | − | − |
D-sorbitol | − | + | − |
D-galactose | + | + | + |
Beta-glucuronidase | − | − | − |
Alpha-mannosidase | − | − | − |
Saccharose/sucrose | + | + | + |
Urease | + | − | + |
Ellman | − | − | − |
Succinate alkalinization | − | − | − |
Phosphatase | − | − | + |
Alpha Glucosidase | − | − | + |
Arginine dihydrolase 2 | − | − | − |
Pullulan | + | + | − |
Bacitracin Resistance | + | − | + |
D-Maltose | + | + | + |
Strain | Strain Code | Zone of Inhibition (mm) | |||||
---|---|---|---|---|---|---|---|
PG (10 µg) | AUG (30 µg) | MZ (5 µg) | AP (10 µg) | CIP (5 µg) | TS (25 µg) | ||
Streptococcus thoraltensi | 27 | 30 (S) | 30 (S) | 0 (R) | 30 (S) | 30 (S) | 30 (S) |
20 | 30 (S) | 33 (S) | 0 (R) | 30 (S) | 30 (S) | 25 (S) | |
Streptococcus alactolyticus | 58 | 19 (S) | 28 (S) | 0 (R) | 23 (S) | 24 (S) | 14 (I) |
48 | 15 (S) | 30 (S) | 0 (R) | 24 (S) | 21 (S) | 25 (S) | |
Streptococcus mutans | 8 | 16 (S) | 30 (S) | 0 (R) | 23 (S) | 26 (S) | 13 (I) |
11 | 22 (S) | 33 (S) | 0 (R) | 30 (S) | 40 (S) | 0 (R) | |
Streptococcus salivarius | 12 | 0 (R) | 0 (R) | 0 (R) | 0 (R) | 0 (R) | 0 (R) |
14 | 0 (R) | 0 (R) | 0 (R) | 0 (R) | 0 (R) | 0 (R) | |
Streptococcus sanguinis | 47 | 12 (R) | 19 (S) | 0 (R) | 17 (S) | 30 (S) | 20 (S) |
30 | 13 (R) | 28 (S) | 0 (R) | 30 (S) | 28 (S) | 15 (S) | |
38 | 14 (R) | 35 (S) | 0 (R) | 28 (S) | 28 (S) | 27 (S) | |
Staphylococcus lentus | 63 | 18 (S) | 28 (S) | 0 (R) | 17 (S) | 25 (S) | 25 (S) |
56 | 17 (S) | 30 (S) | 0 (R) | 28 (S) | 25 (S) | 27 (S) | |
54 | 24 (S) | 35 (S) | 0 (R) | 35 (S) | 25 (S) | 25 (S) | |
49 | 20 (S) | 30 (S) | 0 (R) | 25 (S) | 27 (S) | 30 (S) | |
32 | 20 (S) | 30 (S) | 0 (R) | 30 (S) | 30 (S) | 30 (S) | |
28 | 15 (S) | 30 (S) | 0 (R) | 32 (S) | 27 (S) | 25 (S) | |
Staphylococcus hominis spp. | 36 | 18 (S) | 23 (S) | 0 (R) | 22 (S) | 36 (S) | 22 (S) |
Granulicatella adiacens | 40 | 20 (S) | 30 (S) | 0 (R) | 30 (S) | 30 (S) | 30 (S) |
43 | 15 (S) | 35 (S) | 0 (R) | 33 (S) | 30 (S) | 30 (S) | |
Granulicatella elegans | 25 | 30 (S) | 30 (S) | 0 (R) | 30 (S) | 22 (S) | 30 (S) |
Gemella morbillorum | 60 | 25 (S) | 30 (S) | 0 (R) | 35 (S) | 24 (S) | 25 (S) |
Gemella sanguinis | 65 | 20 (S) | 30 (S) | 0 (R) | 30 (S) | 18 (I) | 20 (S) |
51 | 15 (S) | 28 (S) | 0 (R) | 28 (S) | 25 (S) | 29 (S) | |
Kytococcus | 52 | 0 (R) | 0 (R) | 0 (R) | 0 (R) | 0 (R) | 0 (R) |
Kocuria kristinae | 10 | 0 (R) | 25 (S) | 0 (R) | 0 (R) | 0 (R) | 0 (R) |
62 | 0 (R) | 20 (S) | 0 (R) | 0 (R) | 0 (R) | 0 (R) | |
39 | 12 (R) | 30 (S) | 0 (R) | 0 (R) | 0 (R) | 0 (R) | |
55 | 13(R) | 30(S) | 0(R) | 0(R) | 0(R) | 0 (R) | |
Aerococcus viridans | 50 | 20 (S) | 32 (S) | 0 (R) | 30 (S) | 21 (S) | 25 (S) |
Leuconostoc mesenteroides ssp cremoris | 61 | 20 (S) | 30 (S) | 0 (R) | 30 (S) | 25 (S) | 22 (S) |
Lactobacillus acidophilus | 19 | 25 (S) | 30 (S) | 0 (R) | 30 (S) | 30 (S) | 30 (S) |
64 | 20 (S) | 20 (S) | 0 (R) | 28 (S) | 26 (S) | 32 (S) | |
29 | 25 (S) | 30 (S) | 0 (R) | 30 (S) | 25 (S) | 25 (S) | |
31 | 15 (S) | 26 (S) | 0 (R) | 25 (S) | 25 (S) | 22 (S) | |
33 | 16 (S) | 27 (S) | 0 (R) | 23 (S) | 25 (S) | 20 (S) | |
34 | 15 (S) | 25 (S) | 0 (R) | 25 (S) | 25 (S) | 20 (S) | |
35 | 17 (S) | 20 (S) | 0 (R) | 19 (S) | 25 (S) | 25 (S) | |
37 | 15 (S) | 24 (S) | 0 (R) | 24 (S) | 25 (S) | 24 (S) | |
42 | 25 (S) | 30 (S) | 0 (R) | 25 (S) | 25 (S) | 25 (S) | |
45 | 23 (S) | 35 (S) | 0 (R) | 26 (S) | 25 (S) | 25 (S) | |
46 | 21 (S) | 30 (S) | 0 (R) | 28 (S) | 23 (S) | 26 (S) | |
5 | 20 (S) | 35 (S) | 0 (R) | 17 (S) | 25 (S) | 25 (S) | |
53 | 17 (S) | 33 (S) | 0 (R) | 23 (S) | 23 (S) | 25 (S) | |
57 | 19 (S) | 30 (S) | 0 (R) | 25 (S) | 20 (I) | 22 (S) | |
59 | 16 (S) | 30 (S) | 0 (R) | 27 (S) | 22 (S) | 23 (S) | |
Lactobacillus plantarum | 9 | 0 (R) | 35 (S) | 0 (R) | 30 (S) | 0 (R) | 30 (S) |
Corynebacterium group f-1 | 22 | 15 (S) | 22 (S) | 0 (R) | 22 (S) | 25 (S) | 21 (S) |
Resistant percentage % | 23% | 7% | 100% | 17% | 21% | 21% |
Strain | Patient Number | Cellulose | F | NC | Cellulose/F | NC/F | AgNPs | AgNPs/F | Ag/NC | Ag/NC/F |
---|---|---|---|---|---|---|---|---|---|---|
Streptococcus thoraltensi | 27 | nd | nd | nd | nd | 15 ± 0.28 | 22 ± 0.17 | 19 ± 0.11 | 20 ± 0.57 | 20 ± 0.57 |
20 | nd | nd | nd | 10 ± 0.2 | 20 ± 0.0 | 20 ± 0.0 | 20 ± 0.23 | 21 ± 0.3 | 20 ± 1.15 | |
Streptococcus alactolyticus | 58 | nd | nd | nd | nd | nd | 20 ± 0.72 | 20 ± 0 | 22 ± 0 | 23 ± 0.57 |
48 | nd | nd | nd | 17 ± 0.57 | 20 ± 0.0 | 20 ± 0.0 | 13 ± 0 | 20 ± 0.72 | 20 ± 0 | |
Streptococcus mutans | 8 | nd | nd | nd | 23 ± 0.28 | 22 ± 0.17 | 20 ± 0.72 | 22 ± 0.4 | 24 ± 0.28 | 26 ± 1 |
11 | nd | nd | nd | nd | 20 ± 0.35 | 20 ± 0.11 | 21 ± 0.15 | 18 ± 0.41 | 20 ± 0.57 | |
Streptococcus salivarius | 12 | nd | nd | nd | nd | 16 ± 0.28 | 19 ± 0.0 | 15 ± 0.28 | 20 ± 041 | 22 ± 0.57 |
14 | nd | nd | nd | nd | 15 ± 0.23 | 18 ± 0.25 | 19 ± 0.0 | 14 ± 0.28 | 20 ± 1.15 | |
Streptococcus sanguinis | 47 | nd | nd | nd | nd | 22 ± 0.57 | 20 ± 0.25 | 21 ± 0.0 | 22 ± 0.17 | 25 ± 0.57 |
30 | nd | nd | nd | nd | 13 ± 0.4 | 20 ± 0.0 | 22 ± 0.11 | 22 ± 0.2 | 21 ± 0 | |
38 | nd | nd | nd | nd | 22 ± 0.28 | 20 ± 0.0 | 22 ± 0.17 | 20 ± 0.4 | 23 ± 0.57 | |
Staphylococcus lentus | 63 | nd | nd | nd | nd | 16 ± 0.0 | 22 ± 0.41 | 21 ± 0.45 | 23 ± 0.23 | 23 ± 1 |
56 | nd | nd | nd | nd | nd | 21 ± 0.05 | 22 ± 0.57 | 20 ± 0.5 | 20 ± 0.7 | |
54 | nd | nd | nd | nd | nd | 20 ± 0.23 | 21 ± 0.57 | 20 ± 0 | 22 ± 1.15 | |
49 | nd | nd | nd | nd | nd | 20 ± 0.36 | 23 ± 0.17 | 23 ± 0 | 25 ± 1.15 | |
32 | nd | nd | nd | 10 ± 0.0 | 21 ± 0.28 | 20 ± 0.35 | 22 ± 0.28 | 19 ± 0.23 | 20 ± 0.57 | |
28 | nd | nd | nd | nd | 20 ± 0.2 | 18 ± 0.75 | 20 ± 0.25 | 22 ± 0 | 21 ± 0.14 | |
Granulicatella adiacens | 36 | nd | nd | nd | nd | nd | 23 ± 0.0 | 22 ± 0.48 | 22 ± 0.28 | 25 ± 1.17 |
Granulicatella elegans | 40 | nd | nd | nd | 25 ± 0.28 | 27 ± 0.57 | 23 ± 0.57 | 25 ± 0.46 | 25 ± 0.1 | 22 ± 0.3 |
43 | nd | nd | nd | nd | 29 ± 0.0 | 20 ± 0.28 | 19 ± 0.17 | 25 ± 0 | 27 ± 0.57 | |
Gemella morbillorum | 25 | nd | nd | nd | 19 ± 0.17 | nd | 23 ± 0.23 | 24 ± 0.11 | 20 ± 0.11 | 21 ± 0.8 |
Gemella morbillorum | 60 | nd | nd | nd | nd | nd | 21 ± 0.0 | 22 ± 0.41 | 26 ± 0.23 | 24 ± 0.13 |
Gemella sanguinis | 65 | nd | nd | nd | nd | nd | 2.0 ± 0.57 | 20 ± 0.57 | 21 ± 0.4 | 20 ± 0.57 |
51 | nd | nd | nd | nd | 10 ± 0.28 | 22 ± 0.57 | 15 ± 0.42 | 20 ± 0 | 20 ± 1.15 | |
Kytococcus | 52 | nd | nd | nd | nd | nd | 20 ± 0.46 | 19 ± 0.2 | 21 ± 0 | 23 ± 1.7 |
Kocuria kristinae | 10 | nd | nd | nd | 20 ± 0.14 | 20 ± 0.17 | 15 ± 0.57 | 18 ± 0.25 | 21 ± 0.28 | 21 ± 0.57 |
62 | nd | nd | nd | nd | nd | 20 ± 0.36 | 21 ± 0.057 | 23 ± 00.17 | 25 ± 0.57 | |
39 | nd | nd | nd | nd | nd | 20 ± 0.23 | 2.1 ± 0.23 | 20 ± 0.57 | 20 ± 1.15 | |
55 | nd | nd | nd | nd | nd | 14 ± 0.28 | 13 ± 0.57 | 14 ± 0.26 | 10 ± 0 | |
Aerococcus viridans | 50 | nd | nd | nd | nd | nd | 22 ± 0.41 | 22 ± 0.41 | 12 ± 0.2 | 20 ± 0.28 |
Leuconostoc mesenteroides | 61 | nd | nd | nd | nd | nd | 20 ± 0.20 | 20 ± 0.72 | 20 ± 0.23 | 22 ± 0.28 |
Lactobacillus acidophilus | 19 | nd | nd | nd | nd | nd | 20 ± 0.11 | 22 ± 0.0 | 20 ± 0.36 | 22 ± 0.23 |
64 | nd | nd | nd | 0 | 0 | 19 ± 0.2 | 18 ± 0.0 | 20 ± 0 | 20 ± 0.11 | |
29 | nd | nd | nd | 0 | 0 | 20 ± 0.0 | 20 ± 0.2 | 20 ± 0 | 25 ± 0.46 | |
31 | nd | nd | nd | nd | nd | 20 ± 0.69 | 20 ± 0.35 | 20 ± 0.45 | 12 ± 0.46 | |
33 | nd | nd | nd | nd | nd | 25 ± 0.23 | 23 ± 0.47 | 12 ± 0.0 | 22 ± 0.46 | |
34 | nd | nd | nd | nd | nd | 20 ± 0.51 | 20 ± 0.57 | 20 ± 0.34 | 19 ± 0.4 | |
35 | nd | nd | nd | nd | nd | 20 ± 0.0 | 20 ± 0.41 | 20 ± 0.57 | 17 ± 0.3 | |
37 | nd | nd | nd | nd | nd | 20 ± 0.57 | 20 ± 0.40 | 20 ± 0.2 | 18 ± 0.25 | |
42 | nd | nd | nd | nd | nd | 2.0 ± 0.41 | 19 ± 0.0 | 22 ± 0.0 | 25 ± 0.57 | |
45 | nd | nd | nd | nd | nd | 12 ± 0.0 | 22 ± 0.4 | 25 ± 0.2 | 27 ± 0.4 | |
46 | nd | nd | nd | nd | nd | 24 ± 0.28 | 21 ± 0.11 | 22 ± 0.0 | 23 ± 0.34 | |
5 | nd | nd | nd | nd | nd | 20 ± 0.60 | 20 ± 0.28 | 22 ± 0.11 | 23 ± 0.28 | |
53 | nd | nd | nd | nd | nd | 23 ± 0.28 | 23 ± 0.58 | 25 ± 0.11 | 25 ± 0.28 | |
57 | nd | nd | nd | nd | nd | 20 ± 0.52 | 21 ± 0 | 22 ± 0 | 14 ± 0.82 | |
59 | nd | nd | nd | nd | nd | 22 ± 0.0 | 22 ± 0.28 | 20 ± 0 | 25 ± 0.28 | |
Lactobacillus plantarum | 9 | nd | nd | nd | nd | nd | 20 ± 0.0 | 17 ± 0.58 | 20 ± 0.23 | 21 ± 0.23 |
Corynebacterium group f-1 | 22 | nd | nd | nd | nd | nd | 20 ± 0.57 | 18 ± 0.25 | 20 ± 0.23 | 19 ± 0.57 |
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Hamouda, R.A.; Makharita, R.R.; Qarabai, F.A.K.; Shahabuddin, F.S.; Saddiq, A.A.; Bahammam, L.A.; El-Far, S.W.; Bukhari, M.A.; Elaidarous, M.A.; Abdella, A. Antibacterial Activities of Ag/Cellulose Nanocomposites Derived from Marine Environment Algae against Bacterial Tooth Decay. Microorganisms 2024, 12, 1. https://doi.org/10.3390/microorganisms12010001
Hamouda RA, Makharita RR, Qarabai FAK, Shahabuddin FS, Saddiq AA, Bahammam LA, El-Far SW, Bukhari MA, Elaidarous MA, Abdella A. Antibacterial Activities of Ag/Cellulose Nanocomposites Derived from Marine Environment Algae against Bacterial Tooth Decay. Microorganisms. 2024; 12(1):1. https://doi.org/10.3390/microorganisms12010001
Chicago/Turabian StyleHamouda, Ragaa A., Rabab R. Makharita, Fauzia A. K. Qarabai, Fathi S. Shahabuddin, Amna A. Saddiq, Laila Ahmed Bahammam, Shaymaa W. El-Far, Mamdouh A. Bukhari, Mohammad A. Elaidarous, and Asmaa Abdella. 2024. "Antibacterial Activities of Ag/Cellulose Nanocomposites Derived from Marine Environment Algae against Bacterial Tooth Decay" Microorganisms 12, no. 1: 1. https://doi.org/10.3390/microorganisms12010001