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Microorganisms
Volume 11
Issue 7
10.3390/microorganisms11071712
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Article

Suppression of Nasopharyngeal and Gastric Tumor Growth in a Mouse Model by Antibodies to Epstein–Barr Virus LMP1 Protein

by
Abdelhalim Khenchouche
1,2,
Mounir M. Salem-Bekhit
3,*,
Ahd A. Mansour
4,
Mohammad N. Alomary
5,
Xiaohui Wang
2,
Hayat Ali Alzahrani
6,
Ibrahim M. Al Hosiny
7,
Ehab I. Taha
3,
Gamal A. Shazly
3,
Yacine Benguerba
8,* and
Karim Houali
2,9
1
Département de Microbiologie, Faculté des Sciences de la Nature et de la Vie, Université Ferhat Abbas Sétif 1, Sétif 19000, Algeria
2
Laboratoire de Virologie Moléculaire, FRE3011, CNRS, Faculté de Médecine Laennec, Université Claude Bernard Lyon-1, 69008 Lyon, France
3
Department of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
4
Medical Laboratory Science Department, Fakeeh College for Medical Sciences, P.O. Box 2537, Jeddah 21461, Saudi Arabia
5
Advanced Diagnostic and Therapeutic Institute, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh 11442, Saudi Arabia
6
Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Northern Border University, Arar 73211, Saudi Arabia
7
Microbiology and Immunology Department, Faculty of Medicine, Al-Azhar University, Cairo 11651, Egypt
8
Laboratoire de Biopharmacie Et Pharmacotechnie (LPBT), Ferhat Abbas Setif 1 University, Setif 19000, Algeria
9
Laboratoire de Biochimie Analytique et Biotechnologie (LABAB), Faculté des Sciences Biologiques et des Sciences Agronomiques, Université Mouloud Mammeri, Tizi-Ouzou 15000, Algeria
*
Authors to whom correspondence should be addressed.
Microorganisms 2023, 11(7), 1712; https://doi.org/10.3390/microorganisms11071712
Submission received: 31 May 2023 / Accepted: 21 June 2023 / Published: 30 June 2023
(This article belongs to the Section Molecular Microbiology and Immunology)
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Abstract

The study aimed to investigate the antitumor efficacy of anti-LMP1 antibodies in EBV-positive nasopharyngeal and stomach cell lines and xenograft models. The study also examined the NF-κB expression and cell cycle activation of NPC-serum-exosome-associated LMP1. Anti-LMP1 antibody treatment before or during cell implantation prevented tumor growth in nude mice. A small dose of antibodies resulted in complete tumor regression for at least three months after the tumors had grown in size. The consumption of antigen–antibody complexes by tumor cells limited tumor growth. In vitro experiments showed that anti-LMP1 antibodies killed EBV-positive NPC- or GC-derived epithelial cell lines and EBV-positive human B-cell lines but not EBV-negative cell lines. Treatment with anti-LMP1 reduced NF-κB expression in cells. The animal model experiments showed that anti-LMP1 inhibited and prevented NPC- or GC-derived tumor growth. The results suggest that LMP1 antibody immunotherapy could cure nasopharyngeal cancer, EBV-positive gastric carcinoma, and EBV-associated lymphomas. However, further validation of these findings is required through human clinical trials.
Keywords: EBV oncogenes; LMP1; nasopharyngeal; gastric carcinomas; mouse model; tumor suppression and prevention EBV oncogenes; LMP1; nasopharyngeal; gastric carcinomas; mouse model; tumor suppression and prevention

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MDPI and ACS Style

Khenchouche, A.; Salem-Bekhit, M.M.; Mansour, A.A.; Alomary, M.N.; Wang, X.; Alzahrani, H.A.; Hosiny, I.M.A.; Taha, E.I.; Shazly, G.A.; Benguerba, Y.; et al. Suppression of Nasopharyngeal and Gastric Tumor Growth in a Mouse Model by Antibodies to Epstein–Barr Virus LMP1 Protein. Microorganisms 2023, 11, 1712. https://doi.org/10.3390/microorganisms11071712

AMA Style

Khenchouche A, Salem-Bekhit MM, Mansour AA, Alomary MN, Wang X, Alzahrani HA, Hosiny IMA, Taha EI, Shazly GA, Benguerba Y, et al. Suppression of Nasopharyngeal and Gastric Tumor Growth in a Mouse Model by Antibodies to Epstein–Barr Virus LMP1 Protein. Microorganisms. 2023; 11(7):1712. https://doi.org/10.3390/microorganisms11071712

Chicago/Turabian Style

Khenchouche, Abdelhalim, Mounir M. Salem-Bekhit, Ahd A. Mansour, Mohammad N. Alomary, Xiaohui Wang, Hayat Ali Alzahrani, Ibrahim M. Al Hosiny, Ehab I. Taha, Gamal A. Shazly, Yacine Benguerba, and et al. 2023. "Suppression of Nasopharyngeal and Gastric Tumor Growth in a Mouse Model by Antibodies to Epstein–Barr Virus LMP1 Protein" Microorganisms 11, no. 7: 1712. https://doi.org/10.3390/microorganisms11071712

APA Style

Khenchouche, A., Salem-Bekhit, M. M., Mansour, A. A., Alomary, M. N., Wang, X., Alzahrani, H. A., Hosiny, I. M. A., Taha, E. I., Shazly, G. A., Benguerba, Y., & Houali, K. (2023). Suppression of Nasopharyngeal and Gastric Tumor Growth in a Mouse Model by Antibodies to Epstein–Barr Virus LMP1 Protein. Microorganisms, 11(7), 1712. https://doi.org/10.3390/microorganisms11071712

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