The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity of Ceratocystis fimbriata: Insights from Non-Targeted Metabolomics
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains, Sweet Potato Samples, and Culture Conditions
2.2. Vegetative Growth and Pathogenicity Assays of C. fimbriata
2.3. Ipomeamarone Detection of Sweet Potato
2.4. Ultra-High-Performance Liquid Chromatography-Mass Spectrometry Tests for C. fimbriata
2.5. Potential Metabolites Identification in C. fimbriata
2.6. Potential Differentially Accumulated Metabolites (PDAMs) Analysis
2.7. Statistical Analyses
3. Results and Discussion
3.1. Deletion of CfSWI6 Resulted in Abnormal C. fimbriata Vegetative Growth
3.2. Deletion of CfSWI6 Gene Significantly Reduced the Virulence of C. fimbriata to Sweet Potato
3.3. Disruption of SWI6 Gene Altered the Potential Metabolite Profile of C. fimbriata
3.4. Hierarchical Cluster Analysis Revealed Potential Causes for Abnormal Growth and Pathogenicity in Mutant of C. fimbriata
3.5. Impact of Potential Key Metabolites on Growth and Pathogenicity of C. fimbriata Based on VIP
3.6. SWI6 Mediated the Potential Growth and Virulence of C. fimbriata through Key Metabolic Pathways
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ion Mode | PCA | OPLS-DA | |||
---|---|---|---|---|---|
R2X (cum) | R2Y (cum) | Q2 (cum) | R2 | Q2 | |
POS | 0.608 | 0.983 | 0.925 | 0.8358 | −0.25 |
NEG | 0.644 | 0.984 | 0.943 | 0.7599 | −0.1463 |
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Cong, H.; Li, C.; Wang, Y.; Zhang, Y.; Ma, D.; Li, L.; Jiang, J. The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity of Ceratocystis fimbriata: Insights from Non-Targeted Metabolomics. Microorganisms 2023, 11, 2666. https://doi.org/10.3390/microorganisms11112666
Cong H, Li C, Wang Y, Zhang Y, Ma D, Li L, Jiang J. The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity of Ceratocystis fimbriata: Insights from Non-Targeted Metabolomics. Microorganisms. 2023; 11(11):2666. https://doi.org/10.3390/microorganisms11112666
Chicago/Turabian StyleCong, Hao, Changgen Li, Yiming Wang, Yongjing Zhang, Daifu Ma, Lianwei Li, and Jihong Jiang. 2023. "The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity of Ceratocystis fimbriata: Insights from Non-Targeted Metabolomics" Microorganisms 11, no. 11: 2666. https://doi.org/10.3390/microorganisms11112666