Effects of Light on Growth and Metabolism of Rhodococcus erythropolis

Engelhart-Straub, Selina; Cavelius, Philipp; Hölzl, Fabian; Haack, Martina; Awad, Dania; Brueck, Thomas; Mehlmer, Norbert

doi:10.3390/microorganisms10081680

Open AccessArticle

Effects of Light on Growth and Metabolism of Rhodococcus erythropolis

by

Selina Engelhart-Straub

^†

,

Philipp Cavelius

^†

,

Fabian Hölzl

,

Martina Haack

,

Dania Awad

,

Thomas Brueck

^*

and

Norbert Mehlmer

^*

Werner Siemens-Chair of Synthetic Biotechnology, Department of Chemistry, Technical University of Munich (TUM), 85748 Garching, Germany

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Microorganisms 2022, 10(8), 1680; https://doi.org/10.3390/microorganisms10081680

Submission received: 29 July 2022 / Revised: 16 August 2022 / Accepted: 17 August 2022 / Published: 20 August 2022

(This article belongs to the Special Issue Rhodococcus Species, Their Resistance to Stress and Biotechnological Potential)

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Abstract

Rhodococcus erythropolis is resilient to various stressors. However, the response of R. erythropolis towards light has not been evaluated. In this study, R. erythropolis was exposed to different wavelengths of light. Compared to non-illuminated controls, carotenoid levels were significantly increased in white (standard warm white), green (510 nm) and blue light (470 nm) illuminated cultures. Notably, blue light (455, 425 nm) exhibited anti-microbial effects. Interestingly, cellular lipid composition shifted under light stress, increasing odd chain fatty acids (C15:0, C17:1) cultured under white (standard warm white) and green (510 nm) light. When exposed to blue light (470, 455, 425 nm), fatty acid profiles shifted to more saturated fatty acids (C16:1 to C16:0). Time-resolved proteomics analysis revealed several oxidative stress-related proteins to be upregulated under light illumination.

Keywords: Rhodococcus; photo-oxidative stress; stress response; antimicrobial blue light; carotenoids; fatty acids; time-resolved proteomics

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MDPI and ACS Style

Engelhart-Straub, S.; Cavelius, P.; Hölzl, F.; Haack, M.; Awad, D.; Brueck, T.; Mehlmer, N. Effects of Light on Growth and Metabolism of Rhodococcus erythropolis. Microorganisms 2022, 10, 1680. https://doi.org/10.3390/microorganisms10081680

AMA Style

Engelhart-Straub S, Cavelius P, Hölzl F, Haack M, Awad D, Brueck T, Mehlmer N. Effects of Light on Growth and Metabolism of Rhodococcus erythropolis. Microorganisms. 2022; 10(8):1680. https://doi.org/10.3390/microorganisms10081680

Chicago/Turabian Style

Engelhart-Straub, Selina, Philipp Cavelius, Fabian Hölzl, Martina Haack, Dania Awad, Thomas Brueck, and Norbert Mehlmer. 2022. "Effects of Light on Growth and Metabolism of Rhodococcus erythropolis" Microorganisms 10, no. 8: 1680. https://doi.org/10.3390/microorganisms10081680

APA Style

Engelhart-Straub, S., Cavelius, P., Hölzl, F., Haack, M., Awad, D., Brueck, T., & Mehlmer, N. (2022). Effects of Light on Growth and Metabolism of Rhodococcus erythropolis. Microorganisms, 10(8), 1680. https://doi.org/10.3390/microorganisms10081680

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Effects of Light on Growth and Metabolism of Rhodococcus erythropolis

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