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Article

Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam

1
Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, UK
2
Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK
3
Cell System Modelling Group, Oxford Brookes University, Oxford OX3 0BP, UK
*
Authors to whom correspondence should be addressed.
Academic Editor: Alexander I. Netrusov
Microorganisms 2022, 10(7), 1355; https://doi.org/10.3390/microorganisms10071355
Received: 31 May 2022 / Revised: 23 June 2022 / Accepted: 30 June 2022 / Published: 5 July 2022
Campylobacter jejuni, the major cause of bacterial foodborne illness, is also a fastidious organism that requires strict growth requirements in the laboratory. Our aim was to study substrate utilisation and energy metabolism in non-growing C. jejuni to investigate the ability of these bacteria to survive so effectively in the food chain. We integrated phenotypic microarrays and genome-scale metabolic modelling (GSM) to investigate the survival of C. jejuni on 95 substrates. We further investigated the underlying metabolic re-adjustment associated with varying energy demands on each substrate. We identified amino acids, organic acids and H2, as single substrates supporting survival without growth. We identified several different mechanisms, which were used alone or in combination, for ATP production: substrate-level phosphorylation via acetate kinase, the TCA cycle, and oxidative phosphorylation via the electron transport chain that utilised alternative electron donors and acceptors. The benefit of ATP production through each of these mechanisms was associated with the cost of enzyme investment, nutrient availability and/or O2 utilisation. C. jejuni can utilise a wide range of substrates as energy sources, including organic acids commonly used for marination or preservation of ingredients, which might contribute to the success of their survival in changing environments. View Full-Text
Keywords: Campylobacter jejuni; substrate utilisation; cell survival; energy metabolism; metabolic network Campylobacter jejuni; substrate utilisation; cell survival; energy metabolism; metabolic network
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MDPI and ACS Style

Stoakes, E.; Savva, G.M.; Coates, R.; Tejera, N.; Poolman, M.G.; Grant, A.J.; Wain, J.; Singh, D. Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam. Microorganisms 2022, 10, 1355. https://doi.org/10.3390/microorganisms10071355

AMA Style

Stoakes E, Savva GM, Coates R, Tejera N, Poolman MG, Grant AJ, Wain J, Singh D. Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam. Microorganisms. 2022; 10(7):1355. https://doi.org/10.3390/microorganisms10071355

Chicago/Turabian Style

Stoakes, Emily, George M. Savva, Ruby Coates, Noemi Tejera, Mark G. Poolman, Andrew J. Grant, John Wain, and Dipali Singh. 2022. "Substrate Utilisation and Energy Metabolism in Non-Growing Campylobacter jejuni M1cam" Microorganisms 10, no. 7: 1355. https://doi.org/10.3390/microorganisms10071355

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