Next Article in Journal
A Propidium Monoazide (PMAxx)-Droplet Digital PCR (ddPCR) for the Detection of Viable Burkholderia cepacia Complex in Nuclease-Free Water and Antiseptics
Next Article in Special Issue
Modeling the Impact of Management Changes on the Infection Dynamics of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in the Broiler Production
Previous Article in Journal
Endosymbiotic Bacterial Diversity of Corn Leaf Aphid, Rhopalosiphum maidis Fitch (Hemiptera: Aphididae) Associated with Maize Management Systems
Previous Article in Special Issue
Serbian Traditional Goat Cheese: Physico-Chemical, Sensory, Hygienic and Safety Characteristics
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microorganisms
Volume 10
Issue 5
10.3390/microorganisms10050941
microorganisms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings: A Cross-Sectional Study from the One Health Perspective

by
Vanessa Silva
1,2,3,4,
Susana Correia
1,
Jaqueline Rocha
5,
Célia M. Manaia
5,
Adriana Silva
1,2,3,4,
Juan García-Díez
6,7,
José Eduardo Pereira
6,7,
Teresa Semedo-Lemsaddek
7,8,*,
Gilberto Igrejas
2,3,4,† and
Patrícia Poeta
1,4,6,7,*,†
1
Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
2
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
3
Functional Genomics and Proteomics Unit, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
4
Associated Laboratory for Green Chemistry (LAQV-REQUIMTE), University NOVA of Lisboa, 1099-085 Lisbon, Portugal
5
Centro de Biotecnologia e Química Fina (CBQF), Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, 4169-005 Porto, Portugal
6
Veterinary and Animal Research Centre (CECAV), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
7
Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal
8
Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Avenida da Universidade Técnica, Universidade de Lisboa, 1300-477 Lisboa, Portugal
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2022, 10(5), 941; https://doi.org/10.3390/microorganisms10050941
Submission received: 28 March 2022 / Revised: 21 April 2022 / Accepted: 27 April 2022 / Published: 30 April 2022
(This article belongs to the Special Issue Pathogens and Antimicrobial Drug Resistance in the Food Chain, 2nd Edition)
Browse Figure
Versions Notes

Abstract

:
Staphylococcus aureus have been progressively identified in farm animals and in humans with direct contact with these animals showing that S. aureus may be a major zoonotic pathogen. Therefore, we aimed to isolate S. aureus from cows, their handlers, and their immediate surroundings, and to investigate the antimicrobial resistance and genetic lineages of the isolates. Mouth and nose swabs of 244 healthy cows (195 Maronesa, 11 Holstein-Friesians, and 28 crossbreeds), 82 farm workers, 53 water and 63 soil samples were collected. Identification of species was carried out by MALDI-TOF MS Biotyper. The presence of antimicrobial resistance genes and virulence factors was assessed based on gene search by PCR. All isolates were typed by multilocus sequence typing and spa-typing. From 442 samples, 33 (13.9%), 24 (29.3%), 1 (2%), and 1 (2%) S. aureus were recovered from cows, farm workers, water, and soil samples, respectively. Most of the isolates showed resistance only to penicillin. S. aureus isolates were ascribed to 17 sequence types (STs) and 26 spa-types. Some clonal lineages were common to both cows and farm workers such as ST30-t9413, ST72-t148, and ST45-t350. Through a One Health approach, this study revealed that there is a great diversity of clonal lineages of S. aureus in cows and their handlers. Furthermore, some S. aureus lineages are common to cows and handlers, which may suggest a possible transmission.

1. Introduction

Zoonotic disease events have highlighted the increasing effect of pathogens on human and animal health [1,2]. Therefore, in the past, the One Medicine concept was implemented, which aimed to address animal–human interactions and human and animal health [3]. Later, however, it was evident that the environment was also directly related to human and animal health through, for example, agricultural intensification, climate change, human encroachment into wildlife habitats, and environmental contamination, which were recognized as drivers for zoonotic disease emergence threatening human and animal populations [2,4]. Therefore, a collaborative and multi-disciplinary approach, involving human–animal–environment interactions has been implemented in order to understand the ecology of emerging zoonotic diseases [5]. The One Health concept focuses on the relatedness of human, animal, and environmental health focusing on the emerging zoonoses, food safety, and antimicrobial resistance [5,6,7]. Antimicrobial resistance has been included by the World Health Organization in top ten threats to global health in 2019 and has been recognized as a One Health issue since it can arise in humans, animals, and the environment, and can spread from one compartment to another, between regions and countries [5,8]. A One Health approach to antimicrobial resistance aims not only at understanding this issue, but also how it spreads across hospitals, communities, farming animals, pets, wild animals, wastewaters, and natural water reservoirs [9].
Staphylococcus aureus are part of the skin and mucous membranes of humans and animals, with humans being the main reservoir [10]. However, S. aureus also comprises opportunist bacteria that cause multiple infections, including skin and soft tissue infections, bacteremia, osteomyelitis, endocarditis, among others [11]. S. aureus, particularly methicillin-resistant S. aureus (MRSA), infections have become increasingly difficult to treat due to their ability to easily acquire antimicrobial resistance determinants [12]. In fact, S. aureus is resistant to almost all antimicrobials available so far [13]. Furthermore, S. aureus produces an enormous variety of virulence factors which include a wide range of toxins and immune evasion factors [14]. S. aureus is a widespread species that has been isolated from humans, hospital settings, farm animals, pets, wild animals, wastewater, and surface water [12,15,16,17,18,19,20]. S. aureus isolates can be grouped into different genetic lineages defined by molecular typing methods, such as multilocus sequence typing (MLST), spa-typing, and whole genome sequencing [21]. Epidemiological studies have suggested that these lineages are well adapted to their respective host [22,23]. For instance, several S. aureus clonal complexes (CCs), which are defined by MLST, have been detected in only one animal group as is the case of CC522 and CC385, which have been found only in small ruminants and avian species, respectively [23,24,25]. However, host shifts are a natural feature of S. aureus evolution. S. aureus CCs found in different species may reflect intraspecies transmission or a broad host range [23]. S. aureus isolated from healthy and infected human are mainly represented by CC1, CC5, CC8, CC12, CC15, CC22, CC25, CC30, CC45, CC51, and CC121 [26]. Regarding S. aureus from animals, CC1, CC5, CC9, CC45, CC97, and CC398 are the most frequently detected [22]. However, it is important to point out that some dominant MRSA lineages differ from dominant MSSA lineages in each host [22]. Healthy bovine are carriers of S. aureus mainly in the teat skin, nasal cavity, and rectum [27]. S. aureus, particularly S. aureus CC97, is also a frequent etiological agents of mastitis in cows [27]. Close contact between bovine and farm workers may promote transmission of strains in both ways [28,29]. In fact, studies have shown that CC97 subclades for human infection originated in bovine-to-human host dissemination, which indicates that animals may act as S. aureus reservoirs that can spread to humans [27,30]. The autochthonous Maronesa cattle is a traditional Portuguese breed used for meat production commercialized with PDO—Protected Designation of Origin [31,32]. Maronesa cattle is considered a threatened breed that has been used for centuries in agricultural work [32]. Therefore, in this study, we isolated S. aureus from farm workers, cows, and their environments and aimed to find evidence of bacterial transmission and spread investigating the antimicrobial resistance and genetic lineages of the isolates.

2. Materials and Methods

2.1. Sample Collection

A total of 442 samples were collected from 64 farms in the North of Portugal, which comprises 244 cows (195 Maronesa breed, 11 Holstein-Friesian and 28 crossbreed), 82 farm workers, 53 water samples, and 63 soil samples from February to April 2019. Samples from cows and farm workers were collected with a nasal and mouth swab (one sample per individual). The farms are managed by families who are dedicated to agriculture and generally share the same household. Water samples were collected from the cows’ drinkers using sterile 500 mL plastic bottles with sodium thiosulfate and preserved at 4–8 °C. All samples were filtered on the same day they were collected. Soil samples were collected from the farm grounds with a sterile plastic bag. The age of the cows ranged from 4 to 22 years with an average of 10 years and among the 244 cows, 229 were females, and 15 males (Supplementary Table S1).

2.2. S. aureus Isolation

The swabs and 2 g of soil sample were inserted into tubes containing 5 mL of Brain Heart Infusion (BHI) broth (LiofilChem, Via Scozia, Italy) with 6.5% of NaCl and incubated at 36 °C for 24 h. Then, the inoculum was seeded onto Baird–Parker agar (Oxoid, Basingstoke, UK) plates for S. aureus isolation. Water samples were filtered through a cellulose nitrate 0.45 μm pore membrane filter (Whatman, Maidstone, UK). The filters were then inserted into tubes BHI broth tubes 6.5% of NaCl and incubated at 37 °C for 24 h. After the incubation period, the inoculum was seeded onto Baird–Parker agar plates. Colonies, with S. aureus characteristics but showing morphological differences, were collected from each plate. S. aureus species identification was performed by biochemical tests (catalase, DNase and coagulase) and by MALDI-TOF MS Biotyper (Bruker Daltonics, Billerica, MA, USA).

2.3. Antimicrobial Susceptibility Testing

Antibiotic susceptibility was carried out in all S. aureus isolates and their susceptibility profile was determined using a Kirby–Bauer disk diffusion method against the following 14 antimicrobial agents (concentration/disk; Oxoid, Basingstoke, UK)): penicillin (1U), cefoxitin (30 μg), chloramphenicol (30 μg), ciprofloxacin (5 μg), clindamycin (2 μg), erythromycin (15 μg), fusidic acid (10 μg), gentamicin (10 μg), kanamycin (30 μg), linezolid (10 μg), mupirocin (200 μg), tetracycline (30 μg), tobramycin (10 μg), and trimethoprim/sulfamethoxazole (1.25/23.75 μg). The determination and interpretation of the results was made according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2018) standards except for kanamycin that followed the Clinical and Laboratory Standards Institute guidelines (CLSI, 2017). S. aureus strain ATCC 25923 was used as quality control in all assays.

2.4. Antimicrobial Resistance and Virulence Genes

DNA extraction was performed as previously described using lysostaphin and proteinase K (Sigma Aldrich, St. Louis, MI, USA) [33]. All isolates were screened for the presence antimicrobial resistance genes by PCR and sequencing according to their phenotypic resistance: penicillin (blaZ), aminoglycosides (aac(6′)-Ie-aph(2″)-Ia, aph(3′)-IIIa, ant(4′)-Ia and str), macrolides and lincosamides (ermA, ermB, ermC, ermT, mphC, msr(A/B), lnuA, lnuB, vgaA and vgaB), fusidic acid (fusB, fusC and fusD), tetracyclines (tetM, tetL, tetK and tetO) and chloramphenicol (fexA, fexB, catpC194, catpC221 and catpC223) (Table S2). The presence of the virulence genes lukF/lukS-PV (Panton–Valentine Leukocidin), hla, hlb and hld (alpha-, beta- and delta-hemolysins), eta and etb (exfoliative toxins), and tst (toxic shock syndrome toxin) was also investigated by PCR. In addition, all isolates were screened for the presence of the scn gene, which is a marker of the immune evasion cluster (IEC) system. In isolates positive for scn, the presence of the chp, sak, sea and sep genes was assessed to determine the IEC group [34]. Positive and negative controls used in all experiments belonged to the strain collection of the University of Trás-os-Montes and Alto Douro.

2.5. Molecular Typing

All isolates were typed by MLST, spa-, and agr-typing. The spa region was amplified by PCR, the fragments sequenced, and the obtained sequences were analyzed using Ridom® Staph-type software (version 1.5, Ridom GmbH, Würzburg, Germany) [35]. MLST genotyping was performed as previously described [36]. Allele and STs were determined using the Staphylococcus MLST database at https://pubmlst.org/ (accessed on 7 October 2021). Isolates were also characterized by agr-typing (I–IV) by PCR using specific primers and conditions [37].

3. Results

In this study, S. aureus strains were isolated from cows, farm workers, and the cows’ surrounding environment (soil and water). S. aureus were found in 24 (37.5%) of the 64 farms included in this study. A total of 58 (13.1%) S. aureus were isolated from the 442 samples. From the 244 cows sampled, 32 (13.1%) were colonized by S. aureus. However, one cow co-carried two different lineages of S. aureus; thus, 33 S. aureus were isolated from cows. Among the three tested breeds, Maronesa, Holstein-Friesian and crossbreed, S. aureus were detected in 25. 2 and 6, respectively. Regarding the farm workers, 24 (29.3%) S. aureus were recovered from the 82 samples. Water and soil samples were collected from 53 cows’ drinkers and 63 soil grounds and only one isolate of each origin was recovered.
Table 1 shows the percentage of S. aureus isolates resistant and susceptible to each antibiotic. Farms with positive samples are listed in Table 2 as well as the resistance and virulence profiles, and clonal lineages of the isolates. In 8 (Farm 3, 10, 15, 17, 39, 42, 60 and 63) of the 24 positive farms, S. aureus was isolated from cows only and, in seven farms (Farm 6, 20, 25, 46, 48, 56, and 58), it was isolated from farm workers only. Interestingly, in farms 14 and 55, S. aureus was isolated only from soil and water samples, respectively. In the remaining farms, S. aureus was isolated from both cows and farm workers. All isolates were characterized regarding their antimicrobial resistance, virulence, and clonal lineages. Nineteen isolates from cows (n = 15), farm workers (n = 2), soil (n = 1), and water (n = 1) were susceptible to all antibiotics tested (Table 2). Multidrug resistance was found in one isolate from a cow (VS3222) and one from a human (VS3263). Resistance to penicillin was detected in 36 isolates and all carried the blaZ gene. Four isolates were resistant to aminoglycosides and harbored the aac(6′)-aph(2″) (n = 4), aph(3′)-IIIa (n = 3) and str genes. Two isolates from cows and one from a worker showed resistance to tetracycline conferred by the tetK gene. Resistance to erythromycin was found in four S. aureus isolated from farm workers with two being co-resistant to clindamycin. Resistance to macrolides and lincosamides was encoded by the ermC (n = 2), ermT, and ermB. Only one isolate showed phenotypic resistance to fusidic acid, but none of the tested genes were present. Finally, two S. aureus isolated from a cow and its handler carried the catpC221 gene, which is responsible for chloramphenicol resistance. Five isolates from cows and one from one farm worker were positive for the scn gene of the IEC system and were further investigated regarding the presence of the other IEC genes. The isolates were ascribed to IEC type B (n = 4), G (n = 2) and E. All isolates harbored the virulence genes hla and hld. As expected, the hlb gene was detected in all IEC-negative isolates (n = 56), and six isolates also carried the tst gene. All isolates were typed by MLST, spa- and agr-type. The 58 isolates were affiliated to 18 STs and 26 different spa-types, with 6 and 12 distinct STs and spa-types for the bovine isolates and nine distinct STs and spa-types for human isolates (Figure 1). The most common S. aureus lineage in cows was ST6- t18899 (n = 9/244) and in farm workers was ST30-t012 (n = 7/82). In general, S. aureus isolates from cows were ascribed to ST6 (n = 9), ST133 (n = 5), ST30 (n = 4), ST45 (n = 4), ST72 (n = 3), ST672 (n = 2), ST7464 (n = 2), ST352 (n = 2), ST1 and ST2328, and spa-types t16615 (n = 9), t9413 (n = 4), t18899 (n =3), t148 (n = 3), t959 (n = 2), t871 (n = 2), t3750, t2207, t7355, t7669, t4735, t350, t706, t015, t267, t359 and t563. Isolated from farm workers belonged to ST30 (n = 7), ST45 (n = 4), ST5 (n = 3), ST72, ST121, ST97, ST34, ST188, ST8 and ST398, and spa-types t012 (n = 5), t9413 (n = 2), t018 (n = 2), t045 (n = 2), t189 (n = 2), t148, t7669, t350, t015, t018, t002, t162, t414, t008 and t571. The two S. aureus isolates from water and soil were ascribed to ST30-t018 and ST6-t16615, respectively. As for agr-typing, the isolates were grouped into agr type I (n = 35), II (n = 3), III (n = 19), and IV (n = 1).
Evidence of a possible transmission of S. aureus between farm workers and cows is shown in Farms 16, 62, and 64. For instance, in Farm 16, the same clonal lineage ST30-t9413 in isolates from four cows and one worker, and all isolates have the same phenotype and genotype. In addition, isolates from two farm workers share the same linages which may also suggest a possible human-to-human transmission since workers from the same farm are related and share the same household, and the same is observed in Farm 13. Transmission between cows sharing the same environment may also occur. In Farms 10, 13, and 60, S. aureus from cows share the same clonal lineages among them, which possibly indicates a cow-to-cow transmission.

4. Discussion

Transmission of S. aureus between cows and people working with dairy cattle has been reported in 2007 [38]. Since then, many studies have been published with dairy cattle and the possible transmission between cows and farm workers [29,38,39,40]. However, the great majority of studies focus only on S. aureus as a cause of bovine mastitis or its presence in bovine milk. In fact, S. aureus causing mastitis and the transmission to and from farm workers through direct contact have been extensively studied [29,40]. Indeed, studies investigating the presence of S. aureus in healthy beef cattle and the animal–human–environment transmission in the One Health context are scarce. In our study, we collected a total of 442 samples from cows, farm workers, and the farm environment (soil and water). In our previous study, we reported the absence of MRSA in Maronesa cattle, and so this is the first study reporting the presence of S. aureus in Maronesa cattle, which is an important traditional Portuguese breed [32]. From the 244 cows sampled, 13.1% were colonized by S. aureus, which is higher than most studies conducted with healthy cattle. Other studies conducted with healthy cattle reported an S. aureus frequency of between 5% and 8% [41,42,43]. Moreover, a study carried out in Tunisia reported an even lower frequency of S. aureus of only 1.3% in healthy cattle [44]. Likewise, Garipcin et al. investigated the presence of S. aureus in healthy cattle and humans in close contact with these animals and found a prevalence of 3.2% and 29.3% in cattle and humans, respectively [45]. The results of this study, in relation to samples of human handlers, is the same as that obtained in our study (29.3%). In fact, it has been reported that S. aureus is part of the normal mucosa of around 30% of the human population [46]. In contrast, another study carried out with samples from cattle and their caretakers found S. aureus in 42.9% and 74.2% in cattle and caretakers, respectively, which is a much higher frequency than most studies including ours [47]. Finally, another study similar to ours, in which the presence of S. aureus was investigated in cattle, caretakers, and the farm environment, found S. aureus in 4% and 16.6% of animal and human nose samples, but no S. aureus was found in the environmental samples. S. aureus and MRSA have been reported as environment associated with livestock including pigs, cattle and even in the production chain of dairy products [39,48,49]. In our study, the frequency of S. aureus in soil and water was also very low (1.6 and 1.9%, respectively). However, we excepted a higher frequency of S. aureus in soil samples since studies have shown that environmental sampling of barns and farms may be used for S. aureus and MRSA surveillance in livestock [50,51]. Furthermore, however, there is little information about the survival time of S. aureus on soil, and the manure spread on the farm soil could be a source of S. aureus on soil surfaces. We also expected to find a higher prevalence of S. aureus in the water of the cows’ drinkers since S. aureus is present in the mouth and nose of cows and can spread in the water. This low frequency may be due to the S. aureus survival rate in fresh water, which was reported to be an average of 2.71 days and 4.84 days at 20 °C and 13 °C, respectively [52].
Zoonotic transmission of S. aureus strains between livestock and humans have been reported, particularly, with humans living and working in close contact with a farm [29,39]. S. aureus transmission between cattle and farm workers may occur through direct contact or in indirect exposure through the farm environment [39]. In our study, farm environment contamination did not seem to promote S. aureus colonization in both cattle and farm workers since only two environmental S. aureus were isolated from different farms (farms 14 and 55), and no S. aureus was detected in the cows or in the workers of those farms. Potential transmission between cows and workers was detected in farms 16, 49, 53, and 62. In farm 16, all cows were colonized by S. aureus ST30-t9413 carrying the blaZ, hla, hlb, and hld genes, and one of the farm workers was also colonized by the same S. aureus clone harboring the same genes. In addition, two other workers also carried S. aureus ST30 but with a different spa-type (t018). S. aureus ST30 was the predominant clone found in this study and was detected in cows, humans, and soil samples. ST30 is primarily associated with humans but is also spread among livestock, including cows and pigs [53,54]. Furthermore, CC30 comprises the most common MSSA lineage in Europe and gave rise to important epidemic clones such as EMRSA-16 [55,56]. In this study, ST30 isolates were associated with three spa-types: t018, t9413, and t012. S. aureus from cows were exclusively typed as t9413, while S. aureus ST30 from humans were typed as t018, t9413, and t012. S. aureus ST30-t012 isolate may be related to the Southwest Pacific clone and was the most prevalent clone among community and hospital settings in Portugal between 1992 and 2011 [56]. ST30-t9413 has only been reported in Portugal in strains isolated from wild owls, superficial waters and one farm worker with close contact with cattle, and all studies were conducted in the same region as this study [19,32,57]. spa-type t9413 may be cattle-associated and the ST30-t9413 isolated from farm workers in this study may have an animal origin. Furthermore, CC30 isolates were the only ones carrying the virulence gene tst, but none of the ST30-t9413 harbored this gene. The carriage of tst, in addition to the hemolysins genes, is in accordance with other studies that have shown that S. aureus ST30 often carries pathogenicity islands including tst gene [58]. Other S. aureus isolated in this study belonged to CC30, such as S. aureus ST7464-t871 detected in two cows from farm 39 and S. aureus ST34-t414 isolated from a farm worker (farm 47). Another possible piece of evidence of S. aureus human-to-animal and animal-to-animal transmission was detected in farms 64 and 60, respectively. All S. aureus isolates were typed as ST72 (CC8) and spa-type t148. S. aureus ST72 was first described in South Korea and is a particularly rare clone elsewhere in the world [59]. However, it is mostly associated with MRSA strains frequently found in the community and hospitals [60]. However, MSSA ST72-t148 has also been reported as a common cause of blood infection in Korea [61]. S. aureus ST45 was detected in four cows and four farm workers in this study and associated with five spa-types: t015, t7669, t350, t563, and t706. S. aureus ST45-t7669 was detected in one cow and one farm worker from farm 62 and, since both isolates encode the same resistance and virulence genes, we can suggest a possible bacterial transmission. S. aureus ST45 is a human-associated clone and is a major global MRSA lineage [62]. Nevertheless, MSSA ST45 has been detected in cow mastitis and farm workers with direct contact [63,64]. Effelsberg et al. analyzed a large collection of ST45 isolates from six continents and reported that ST45 phylogeny is defined by two distinct sublineages which correlated with geographical origins of the isolates [62]. However, in our study, since 3 of the 4 ST45 isolates from cows carried the IEC system genes, we can suggest that it may indicate a human spillover rather than an animal-associated ST45 sublineage as previously stated [62]. S. aureus ST6-t16615 was the most prevalent lineage in cows and was not detected in human samples. This lineage has been reported among wild rats and owls in Portugal [57,65] and as the main lineage in livestock in Algeria [66]. Although considered a human clone with relatively high prevalence in Asian countries, this lineage seems to be widely disseminated among animals [67]. Furthermore, in our study, none of the isolates carried the IEC systems, which suggests a possible animal adaptation. Some of the remaining S. aureus lineages were only detected in cows: ST133, ST672, ST352, ST1, and ST2328. S. aureus ST133 and ST2328 belonging to CC133 and ST1 (CC1) are known to be livestock-associated and lately have emerged as important zoonotic lineages [68]. CC133 lineage is regarded as mostly ungulate-animal specific, but it has also been detected in wild animals and surface waters [19,57,69]. In fact, S. aureus CC133 has been reported as the most prevalent in bovine mastitis milk [70,71]. spa-type t18899, found in three ST133 isolates in our study, was only reported in milk samples [72]. ST672 lineage is an emerging strain from the Indian subcontinent often related with CA-MRSA and rarely found elsewhere [73]. In our study, both ST672 isolates carried the IEC genes and were ascribed to group G, which may confirm a human origin [68]. S. aureus ST352 belongs to CC97, which is an animal-specific lineage, but it has also been detected in one farm worker in this study. CC97 is a pandemic bovine S. aureus lineage that emerged as a zoonotic agent and has been reported as a human epidemic CA-MRSA after host adaptation [30,47]. Other S. aureus lineages were exclusively detected in farm workers such as ST5, ST121, ST188, ST8, and ST398. ST5, ST8, and ST188 classical human linages [47]. However, ST398-t571 is the most common livestock-associated S. aureus lineage in Europe [74]. As in animals, the spa-type t571 is the most common spa-type in MSSA ST398 in humans [75]. However, this isolate has characteristics typical of being of animal origin: it has resistance to tetracycline conferred by the tetK gene, which is known to be a livestock-associated marker, and lacks the IEC system genes, which is currently considered to be the marker for human host adaptation [76]. References [77,78,79,80,81,82,83,84,85,86,87,88,89,90,91] are cited in the Supplementary Materials.

5. Conclusions

In this study, both cows and farm workers are carriers of S. aureus strains. However, S. aureus was isolated from only one soil and one water sample, which may suggest a low survival of S. aureus in the environment. Several cow isolates that belonged to classical human genetic lineages were indistinguishable from S. aureus isolated from farm workers in close contact with the cows, which suggests a possible transmission from humans as previously evoked. Animal-to-human transmission may have also occurred, although in a smaller number of cases, which indicates an acquisition through occupational contact. Moreover, our results also provide the evidence of S. aureus transmission among cows and among humans sharing the same household, although the direction of transfer could not be proven.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms10050941/s1, Table S1: Farms, date, and location of sample collection and distribution of the S. aureus isolates among cows, farmers, and environment samples; Table S2: Primer pairs used for molecular typing and detection of antimicrobial resistance genes in S. aureus strains.

Author Contributions

Conceptualization, S.C. and P.P.; methodology, V.S. and S.C.; validation, J.E.P., T.S.-L., and P.P.; investigation, V.S., S.C., and J.R.; resources, J.G.-D.; data curation, V.S. and A.S.; writing—original draft preparation, V.S.; writing—review and editing, V.S., J.R., and C.M.M.; supervision, G.I. and P.P. All authors have read and agreed to the published version of the manuscript.

Funding

This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with references NORTE-01-0145-FEDER-030101 and PTDC/SAU-INF/30101/2017, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT/MCTES (UIDB/50006/2020 and UIDP/50006/2020) and by the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). Vanessa Silva is grateful to FCT (Fundacão para a Ciência e a Tecnologia) for financial support through the PhD grant SFRH/BD/137947/2018.

Institutional Review Board Statement

The study was conducted according to the Helsinki Declaration (ICH-GCP principles), in compliance with Schedule Y/ICMR Guidelines, the Oviedo Convention, and was approved by the Ethics Committee of University of Trás-os-Montes e Alto Douro (EC-UTAD, 8 November 2019).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Chambers, H.F.; Deleo, F.R. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat. Rev. Microbiol. 2009, 7, 629–641. [Google Scholar] [CrossRef] [PubMed]
  2. Rabinowitz, P.M.; Kock, R.; Kachani, M.; Kunkel, R.; Thomas, J.; Gilbert, J.; Wallace, R.; Blackmore, C.; Wong, D.; Karesh, W.; et al. Toward proof of concept of a one health approach to disease prediction and control. Emerg. Infect. Dis. 2013, 19, e130265. [Google Scholar] [CrossRef] [PubMed]
  3. Schwabe, C.W. The challenge of “one medicine”. In Veterinary Medicine and Human Health, 3rd ed.; Williams & Wilkins: Baltimore, MD, USA, 1984; pp. 1–15. [Google Scholar]
  4. Rabinowitz, P.; Scotch, M.; Conti, L. Human and animal sentinels for shared health risks. Vet. Ital. 2009, 45, 23. [Google Scholar] [PubMed]
  5. Mackenzie, J.S.; Jeggo, M. The One Health Approach—Why Is It So Important? Trop. Med. Infect. Dis. 2019, 4, 88. [Google Scholar] [CrossRef] [Green Version]
  6. Boqvist, S.; Söderqvist, K.; Vågsholm, I. Food safety challenges and One Health within Europe. Acta Vet. Scand. 2018, 60, 1. [Google Scholar] [CrossRef]
  7. Ceric, O.; Tyson, G.H.; Goodman, L.B.; Mitchell, P.K.; Zhang, Y.; Prarat, M.; Cui, J.; Peak, L.; Scaria, J.; Antony, L. Enhancing the one health initiative by using whole genome sequencing to monitor antimicrobial resistance of animal pathogens: Vet-LIRN collaborative project with veterinary diagnostic laboratories in United States and Canada. BMC Vet. Res. 2019, 15, 130. [Google Scholar] [CrossRef] [Green Version]
  8. World Health Organization. Ten Threats to Global Health in 2019; World Health Organization: Geneva, Switzerland, 2019.
  9. More, S.J. European perspectives on efforts to reduce antimicrobial usage in food animal production. Ir. Vet. J. 2020, 73, 2. [Google Scholar] [CrossRef] [Green Version]
  10. Taylor, T.A.; Unakal, C.G. Staphylococcus aureus. Available online: https://www.statpearls.com/ (accessed on 9 December 2021).
  11. Tong, S.Y.C.; Davis, J.S.; Eichenberger, E.; Holland, T.L.; Fowler, V.G., Jr. Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management. Clin. Microbiol. Rev. 2015, 28, 603–661. [Google Scholar] [CrossRef] [Green Version]
  12. Silva, V.; Capelo, J.L.; Igrejas, G.; Poeta, P. Molecular Epidemiology of Staphylococcus aureus Lineages in Wild Animals in Europe: A Review. Antibiotics 2020, 9, 122. [Google Scholar] [CrossRef] [Green Version]
  13. Mukherjee, R. Antimicrobial Resistance in Staphylococcus aureus; Priyadarshini, A., Ed.; IntechOpen: Rijeka, Croatia, 2021; Chapter 5; ISBN 978-1-83962-743-9. [Google Scholar]
  14. Cheung, G.Y.C.; Bae, J.S.; Otto, M. Pathogenicity and virulence of Staphylococcus aureus. Virulence 2021, 12, 547–569. [Google Scholar] [CrossRef]
  15. Sieber, R.N.; Skov, R.L.; Nielsen, J.; Schulz, J.; Price, L.B.; Aarestrup, F.M.; Larsen, A.R.; Stegger, M.; Larsen, J. Drivers and dynamics of methicillin-resistant livestock-associated Staphylococcus aureus CC398 in pigs and humans in Denmark. MBio 2018, 9, e02142-18. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Hogan, P.G.; Mork, R.L.; Boyle, M.G.; Muenks, C.E.; Morelli, J.J.; Thompson, R.M.; Sullivan, M.L.; Gehlert, S.J.; Merlo, J.R.; McKenzie, M.G. Interplay of personal, pet, and environmental colonization in households affected by community-associated methicillin-resistant Staphylococcus aureus. J. Infect. 2019, 78, 200–207. [Google Scholar] [CrossRef] [PubMed]
  17. Adamu, J.Y.; Raufu, A.I.; Chimaroke, F.C.; Ameh, J.A. Antimicrobial susceptibility testing of Staphylococcus aureus isolated from apparently healthy humans and animals in Maiduguri, Nigeria. Int. J. Biomed. Health Sci. 2021, 6, 191–195. [Google Scholar]
  18. Kearney, A.; Kinnevey, P.; Shore, A.; Earls, M.; Poovelikunnel, T.T.; Brennan, G.; Humphreys, H.; Coleman, D.C. The oral cavity revealed as a significant reservoir of Staphylococcus aureus in an acute hospital by extensive patient, healthcare worker and environmental sampling. J. Hosp. Infect. 2020, 105, 389–396. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  19. Silva, V.; Ferreira, E.; Manageiro, V.; Reis, L.; Tejedor-Junco, M.T.; Sampaio, A.; Capelo, J.L.; Caniça, M.; Igrejas, G.; Poeta, P. Distribution and Clonal Diversity of Staphylococcus aureus and Other Staphylococci in Surface Waters: Detection of ST425-t742 and ST130-t843 mecC-Positive MRSA Strains. Antibiotics 2021, 10, 1416. [Google Scholar] [CrossRef]
  20. Silva, V.; Ribeiro, J.; Rocha, J.; Manaia, C.M.; Silva, A.; Pereira, J.E.; Maltez, L.; Capelo, J.L.; Igrejas, G.; Poeta, P. High Frequency of the EMRSA-15 Clone (ST22-MRSA-IV) in Hospital Wastewater. Microorganisms 2022, 10, 147. [Google Scholar] [CrossRef]
  21. Silva, V.; Caniça, M.; Capelo, J.L.; Igrejas, G.; Poeta, P. Diversity and genetic lineages of environmental staphylococci: A surface water overview. FEMS Microbiol. Ecol. 2020, 96, fiaa191. [Google Scholar] [CrossRef]
  22. McCarthy, A.J.; Lindsay, J.A.; Loeffler, A. Are all meticillin-resistant Staphylococcus aureus (MRSA) equal in all hosts? Epidemiological and genetic comparison between animal and human MRSA. Vet. Dermatol. 2012, 23, 267-e54. [Google Scholar] [CrossRef]
  23. Matuszewska, M.; Murray, G.G.R.; Harrison, E.M.; Holmes, M.A.; Weinert, L.A. The Evolutionary Genomics of Host Specificity in Staphylococcus aureus. Trends Microbiol. 2020, 28, 465–477. [Google Scholar] [CrossRef]
  24. Smith, E.M.; Needs, P.F.; Manley, G.; Green, L.E. Global distribution and diversity of ovine-associated Staphylococcus aureus. Infect. Genet. Evol. 2014, 22, 208–215. [Google Scholar] [CrossRef] [Green Version]
  25. Murray, S.; Pascoe, B.; Meric, G.; Mageiros, L.; Yahara, K.; Hitchings, M.D.; Friedmann, Y.; Wilkinson, T.S.; Gormley, F.J.; Mack, D. Recombination-mediated host adaptation by avian Staphylococcus aureus. Genome Biol. Evol. 2017, 9, 830–842. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  26. Shepheard, M.A.; Fleming, V.M.; Connor, T.R.; Corander, J.; Feil, E.J.; Fraser, C.; Hanage, W.P. Historical zoonoses and other changes in host tropism of Staphylococcus aureus, identified by phylogenetic analysis of a population dataset. PLoS ONE 2013, 8, e62369. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  27. Haag, A.F.; Fitzgerald, J.R.; Penadés, J.R. Staphylococcus aureus in Animals. Gram-Positive Pathog. 2019, 7, 731–746. [Google Scholar]
  28. Resch, G.; François, P.; Morisset, D.; Stojanov, M.; Bonetti, E.J.; Schrenzel, J.; Sakwinska, O.; Moreillon, P. Human-to-bovine jump of Staphylococcus aureus CC8 is associated with the loss of a β-hemolysin converting prophage and the acquisition of a new staphylococcal cassette chromosome. PLoS ONE 2013, 8, e58187. [Google Scholar] [CrossRef]
  29. Krukowski, H.; Bakuła, Z.; Iskra, M.; Olender, A.; Bis-Wencel, H.; Jagielski, T. The first outbreak of methicillin-resistant Staphylococcus aureus in dairy cattle in Poland with evidence of on-farm and intrahousehold transmission. J. Dairy Sci. 2020, 103, 10577–10584. [Google Scholar] [CrossRef]
  30. Spoor, L.E.; McAdam, P.R.; Weinert, L.A.; Rambaut, A.; Hasman, H.; Aarestrup, F.M.; Kearns, A.M.; Larsen, A.R.; Skov, R.L.; Fitzgerald, J.R. Livestock origin for a human pandemic clone of community-associated methicillin-resistant Staphylococcus aureus. MBio 2013, 4, e00356-13. [Google Scholar] [CrossRef] [Green Version]
  31. Garcia, J.E.Y. Catálogo de Raças Autóctones de Castela e Leão (Espanha); Rei Afonso Henriques Foundation: Salamanca, Spain, 2002. [Google Scholar]
  32. Correia, S.; Silva, V.; García-Díez, J.; Teixeira, P.; Pimenta, K.; Pereira, J.E.; Oliveira, S.; Rocha, J.; Manaia, C.M.; Igrejas, G.; et al. One Health Approach Reveals the Absence of Methicillin-Resistant Staphylococcus aureus in Autochthonous Cattle and Their Environments. Front. Microbiol. 2019, 10, 2735. [Google Scholar] [CrossRef] [Green Version]
  33. Silva, V.; Caniça, M.; Ferreira, E.; Vieira-Pinto, M.; Saraiva, C.; Pereira, J.E.; Capelo, J.L.; Igrejas, G.; Poeta, P. Multidrug-Resistant Methicillin-Resistant Coagulase-Negative Staphylococci in Healthy Poultry Slaughtered for Human Consumption. Antibiotics 2022, 11, 365. [Google Scholar] [CrossRef]
  34. Van Wamel, W.J.B.; Rooijakkers, S.H.M.; Ruyken, M.; van Kessel, K.P.M.; van Strijp, J.A.G. The innate immune modulators staphylococcal complement inhibitor and chemotaxis inhibitory protein of Staphylococcus aureus are located on beta-hemolysin-converting bacteriophages. J. Bacteriol. 2006, 188, 1310–1315. [Google Scholar] [CrossRef] [Green Version]
  35. Harmsen, D.; Claus, H.; Witte, W.; Rothganger, J.; Claus, H.; Turnwald, D.; Vogel, U. Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management. J. Clin. Microbiol. 2003, 41, 5442–5448. [Google Scholar] [CrossRef] [Green Version]
  36. Enright, M.C.; Day, N.P.; Davies, C.E.; Peacock, S.J.; Spratt, B.G. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 2000, 38, 1008–1015. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Shopsin, B.; Mathema, B.; Alcabes, P.; Said-Salim, B.; Lina, G.; Matsuka, A.; Martinez, J.; Kreiswirth, B.N. Prevalence of agr specificity groups among Staphylococcus aureus strains colonizing children and their guardians. J. Clin. Microbiol. 2003, 41, 456–459. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  38. Juhász-Kaszanyitzky, E.; Jánosi, S.; Somogyi, P.; Dán, A.; van der Graaf-van Bloois, L.; van Duijkeren, E.; Wagenaar, J.A. MRSA transmission between cows and humans. Emerg. Infect. Dis. 2007, 13, 630–632. [Google Scholar] [CrossRef] [PubMed]
  39. Locatelli, C.; Cremonesi, P.; Caprioli, A.; Carfora, V.; Ianzano, A.; Barberio, A.; Morandi, S.; Casula, A.; Castiglioni, B.; Bronzo, V.; et al. Occurrence of methicillin-resistant Staphylococcus aureus in dairy cattle herds, related swine farms, and humans in contact with herds. J. Dairy Sci. 2017, 100, 608–619. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  40. Schmidt, T.; Kock, M.M.; Ehlers, M.M. Molecular Characterization of Staphylococcus aureus Isolated from Bovine Mastitis and Close Human Contacts in South African Dairy Herds: Genetic Diversity and Inter-Species Host Transmission. Front. Microbiol. 2017, 8, 511. [Google Scholar] [CrossRef] [Green Version]
  41. Santos, R.P.; Souza, F.N.; Oliveira, A.C.D.; de Souza Filho, A.F.; Aizawa, J.; Moreno, L.Z.; da Cunha, A.F.; Cortez, A.; Della Libera, A.M.M.P.; Heinemann, M.B.; et al. Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples. Animals 2020, 10, 2143. [Google Scholar] [CrossRef]
  42. Rahimi, H.; Dastmalchi Saei, H.; Ahmadi, M. Nasal Carriage of Staphylococcus aureus: Frequency and Antibiotic Resistance in Healthy Ruminants. Jundishapur J. Microbiol. 2015, 8, e22413. [Google Scholar] [CrossRef] [Green Version]
  43. Khemiri, M.; Abbassi, M.S.; Couto, N.; Mansouri, R.; Hammami, S.; Pomba, C. Genetic characterisation of Staphylococcus aureus isolated from milk and nasal samples of healthy cows in Tunisia: First report of ST97-t267-agrI-SCCmecV MRSA of bovine origin in Tunisia. J. Glob. Antimicrob. Resist. 2018, 14, 161–165. [Google Scholar] [CrossRef]
  44. Gharsa, H.; Slama, K.B.; Gómez-Sanz, E.; Lozano, C.; Zarazaga, M.; Messadi, L.; Boudabous, A.; Torres, C. Molecular Characterization of Staphylococcus aureus from Nasal Samples of Healthy Farm Animals and Pets in Tunisia. Vector-Borne Zoonotic Dis. 2015, 15, 109–115. [Google Scholar] [CrossRef]
  45. Garipcin, M.; Seker, E. Nasal carriage of methicillin-resistant Staphylococcus aureus in cattle and farm workers in Turkey. Vet. Arh. 2015, 85, 117–129. [Google Scholar]
  46. Claudia, L.; Andreas, P.; Bernhard, K. Staphylococcus aureus Colonization of the Human Nose and Interaction with Other Microbiome Members. Microbiol. Spectr. 2019, 7, 2–7. [Google Scholar] [CrossRef]
  47. El-Ashker, M.; Monecke, S.; Gwida, M.; Saad, T.; El-Gohary, A.; Mohamed, A.; Reißig, A.; Frankenfeld, K.; Gary, D.; Müller, E.; et al. Molecular characterisation of methicillin-resistant and methicillin-susceptible Staphylococcus aureus clones isolated from healthy dairy animals and their caretakers in Egypt. Vet. Microbiol. 2022, 267, 109374. [Google Scholar] [CrossRef] [PubMed]
  48. Davis, M.F.; Pisanic, N.; Rhodes, S.M.; Brown, A.; Keller, H.; Nadimpalli, M.; Christ, A.; Ludwig, S.; Ordak, C.; Spicer, K.; et al. Occurrence of Staphylococcus aureus in swine and swine workplace environments on industrial and antibiotic-free hog operations in North Carolina, USA: A One Health pilot study. Environ. Res. 2018, 163, 88–96. [Google Scholar] [CrossRef]
  49. Papadopoulos, P.; Papadopoulos, T.; Angelidis, A.S.; Boukouvala, E.; Zdragas, A.; Papa, A.; Hadjichristodoulou, C.; Sergelidis, D. Prevalence of Staphylococcus aureus and of methicillin-resistant S. aureus (MRSA) along the production chain of dairy products in north-western Greece. Food Microbiol. 2018, 69, 43–50. [Google Scholar] [CrossRef]
  50. Peterson, A.E.; Davis, M.F.; Awantang, G.; Limbago, B.; Fosheim, G.E.; Silbergeld, E.K. Correlation between animal nasal carriage and environmental methicillin-resistant Staphylococcus aureus isolates at U.S. horse and cattle farms. Vet. Microbiol. 2012, 160, 539–543. [Google Scholar] [CrossRef]
  51. Schulz., J.; Friese, A.; Klees, S.; Tenhagen, B.A.; Fetsch, A.; Rösler, U.; Hartung, H. Longitudinal Study of the Contamination of Air and of Soil Surfaces in the Vicinity of Pig Barns by Livestock-Associated Methicillin-Resistant Staphylococcus aureus. Appl. Environ. Microbiol. 2012, 78, 5666–5671. [Google Scholar] [CrossRef] [Green Version]
  52. Levin-Edens, E.; Bonilla, N.; Meschke, J.S.; Roberts, M.C. Survival of environmental and clinical strains of methicillin-resistant Staphylococcus aureus [MRSA] in marine and fresh waters. Water Res. 2011, 45, 5681–5686. [Google Scholar] [CrossRef]
  53. Mechesso, A.F.; Kim, S.-J.; Park, H.-S.; Choi, J.-H.; Song, H.-J.; Kim, M.H.; Lim, S.; Yoon, S.-S.; Moon, D.-C. First detection of Panton-Valentine leukocidin–positive methicillin-resistant Staphylococcus aureus ST30 in raw milk taken from dairy cows with mastitis in South Korea. J. Dairy Sci. 2021, 104, 969–976. [Google Scholar] [CrossRef]
  54. Simon, A.C.; Baldo, V.; Losio, N.; Filipello, V.; Colagiorgi, A.; Scali, F.; Zanardi, E.; Ghidini, S.; Ianieri, A.; Alborali, G.L. Molecular characterization of Methicillin-resistant Staphylococcus aureus isolated from the pig production chain in Northern Italy. Ital. J. Food Saf. 2020, 9, 8412. [Google Scholar] [CrossRef]
  55. Aanensen, D.M.; Feil, E.J.; Holden, M.T.G.; Dordel, J.; Yeats, C.A.; Fedosejev, A.; Goater, R.; Castillo-Ramírez, S.; Corander, J.; Colijn, C. Whole-genome sequencing for routine pathogen surveillance in public health: A population snapshot of invasive Staphylococcus aureus in Europe. MBio 2016, 7, e00444-16. [Google Scholar] [CrossRef] [Green Version]
  56. Tavares, A.; Faria, N.A.; de Lencastre, H.; Miragaia, M. Population structure of methicillin-susceptible Staphylococcus aureus (MSSA) in Portugal over a 19-year period (1992–2011). Eur. J. Clin. Microbiol. Infect. Dis. 2014, 33, 423–432. [Google Scholar] [CrossRef]
  57. Silva, V.; Lopes, A.F.; Soeiro, V.; Caniça, M.; Manageiro, V.; Pereira, J.E.; Maltez, L.; Capelo, J.L.; Igrejas, G.; Poeta, P. Nocturnal Birds of Prey as Carriers of Staphylococcus aureus and Other Staphylococci: Diversity, Antimicrobial Resistance and Clonal Lineages. Antibiotics 2022, 11, 240. [Google Scholar] [CrossRef]
  58. Papadimitriou-Olivgeris, M.; Drougka, E.; Fligou, F.; Dodou, V.; Kolonitsiou, F.; Filos, K.S.; Anastassiou, E.D.; Petinaki, E.; Marangos, M.; Spiliopoulou, I. Spread of Tst–Positive Staphylococcus aureus Strains Belonging to ST30 Clone among Patients and Healthcare Workers in Two Intensive Care Units. Toxins 2017, 9, 270. [Google Scholar] [CrossRef] [Green Version]
  59. Park, C.; Lee, D.-G.; Kim, S.W.; Choi, S.-M.; Park, S.H.; Chun, H.-S.; Choi, J.-H.; Yoo, J.-H.; Shin, W.S.; Kang, J.H. Predominance of community-associated methicillin-resistant Staphylococcus aureus strains carrying staphylococcal chromosome cassette mec type IVA in South Korea. J. Clin. Microbiol. 2007, 45, 4021–4026. [Google Scholar] [CrossRef] [Green Version]
  60. Salgueiro, V.; Manageiro, V.; Bandarra, N.M.; Ferreira, E.; Clemente, L.; Caniça, M. Genetic Relatedness and Diversity of Staphylococcus aureus from Different Reservoirs: Humans and Animals of Livestock, Poultry, Zoo, and Aquaculture. Microorganisms 2020, 8, 1345. [Google Scholar] [CrossRef]
  61. Hyukmin, L.; Eun-Jeong, Y.; Dokyun, K.; Wook, K.J.; Kwang-Jun, L.; Soo, K.H.; Ree, K.Y.; Hee, S.J.; Hwan, S.J.; Seob, S.K.; et al. Ceftaroline Resistance by Clone-Specific Polymorphism in Penicillin-Binding Protein 2a of Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 2022, 62, e00485-18. [Google Scholar] [CrossRef] [Green Version]
  62. Effelsberg, N.; Stegger, M.; Peitzmann, L.; Altinok, O.; Coombs, G.W.; Pichon, B.; Kearns, A.; Randad, P.R.; Heaney, C.D.; Bletz, S.; et al. Global Epidemiology and Evolutionary History of Staphylococcus aureus ST45. J. Clin. Microbiol. 2022, 59, e02198-20. [Google Scholar] [CrossRef]
  63. Dastmalchi Saei, H.; Panahi, M. Genotyping and antimicrobial resistance of Staphylococcus aureus isolates from dairy ruminants: Differences in the distribution of clonal types between cattle and small ruminants. Arch. Microbiol. 2020, 202, 115–125. [Google Scholar] [CrossRef]
  64. Akkou, M.; Bouchiat, C.; Antri, K.; Bes, M.; Tristan, A.; Dauwalder, O.; Martins-Simoes, P.; Rasigade, J.-P.; Etienne, J.; Vandenesch, F.; et al. New host shift from human to cows within Staphylococcus aureus involved in bovine mastitis and nasal carriage of animal’s caretakers. Vet. Microbiol. 2018, 223, 173–180. [Google Scholar] [CrossRef]
  65. Silva, V.; Gabriel, S.I.; Borrego, S.B.; Tejedor-Junco, M.T.; Manageiro, V.; Ferreira, E.; Reis, L.; Caniça, M.; Capelo, J.L.; Igrejas, G.; et al. Antimicrobial Resistance and Genetic Lineages of Staphylococcus aureus from Wild Rodents: First Report of mecC-Positive Methicillin-Resistant S. aureus (MRSA) in Portugal. Animals 2021, 11, 1537. [Google Scholar] [CrossRef]
  66. Mairi, A.; Touati, A.; Pantel, A.; Zenati, K.; Martinez, A.Y.; Dunyach-Remy, C.; Sotto, A.; Lavigne, J.-P. Distribution of Toxinogenic Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus from Different Ecological Niches in Algeria. Toxins 2019, 11, 500. [Google Scholar] [CrossRef] [Green Version]
  67. Goudarzi, M.; Goudarzi, H.; Sá Figueiredo, A.M.; Udo, E.E.; Fazeli, M.; Asadzadeh, M.; Seyedjavadi, S.S. Molecular Characterization of Methicillin Resistant Staphylococcus aureus Strains Isolated from Intensive Care Units in Iran: ST22-SCCmec IV/t790 Emerges as the Major Clone. PLoS ONE 2016, 11, e0155529. [Google Scholar] [CrossRef] [Green Version]
  68. Benito, D.; Gómez, P.; Aspiroz, C.; Zarazaga, M.; Lozano, C.; Torres, C. Molecular characterization of Staphylococcus aureus isolated from humans related to a livestock farm in Spain, with detection of MRSA-CC130 carrying mecC gene: A zoonotic case? Enferm. Infecc. Microbiol. Clin. 2016, 34, 280–285. [Google Scholar] [CrossRef]
  69. Seinige, D.; Von Altrock, A.; Kehrenberg, C. Genetic diversity and antibiotic susceptibility of Staphylococcus aureus isolates from wild boars. Comp. Immunol. Microbiol. Infect. Dis. 2017, 54, 7–12. [Google Scholar] [CrossRef]
  70. Sheet, O.H.; Grabowski, N.T.; Klein, G.; Reich, F.; Abdulmawjood, A. Characterisation of mecA gene negative Staphylococcus aureus isolated from bovine mastitis milk from Northern Germany. Folia Microbiol. 2019, 64, 845–855. [Google Scholar] [CrossRef]
  71. Cvetnić, L.; Samardžija, M.; Duvnjak, S.; Habrun, B.; Cvetnić, M.; Jaki Tkalec, V.; Đuričić, D.; Benić, M. Multi Locus Sequence Typing and spa Typing of Staphylococcus aureus Isolated from the Milk of Cows with Subclinical Mastitis in Croatia. Microorganisms 2021, 9, 725. [Google Scholar] [CrossRef]
  72. Kroning, I.S.; Haubert, L.; Kleinubing, N.R.; Jaskulski, I.B.; Scheik, L.K.; Ramires, T.; da Silva, W.P. New spa types, resistance to sanitisers and presence of efflux pump genes in Staphylococcus aureus from milk. Int. Dairy J. 2020, 109, 104712. [Google Scholar] [CrossRef]
  73. Schaumburg, F.; Köck, R.; Leendertz, F.H.; Becker, K. Airport door handles and the global spread of antimicrobial-resistant bacteria: A cross sectional study. Clin. Microbiol. Infect. 2016, 22, 1010–1011. [Google Scholar] [CrossRef]
  74. Ji, X.; Krüger, H.; Feßler, A.T.; Liu, J.; Zeng, Z.; Wang, Y.; Wu, C.; Schwarz, S. A novel SCCmec type V variant in porcine MRSA ST398 from China. J. Antimicrob. Chemother. 2020, 75, 484–486. [Google Scholar] [CrossRef]
  75. Tegegne, H.A.; Madec, J.-Y.; Haenni, M. Is methicillin-susceptible Staphylococcus aureus (MSSA) CC398 a true animal-independent pathogen? J. Glob. Antimicrob. Resist. 2022, 29, 120–123. [Google Scholar] [CrossRef]
  76. Tegegne, H.A.; Florianová, M.; Gelbíčová, T.; Karpíšková, R.; Koláčková, I. Detection and molecular characterization of methicillin-resistant Staphylococcus aureus isolated from bulk tank milk of cows, sheep, and goats. Foodborne Pathog. Dis. 2019, 16, 68–73. [Google Scholar] [CrossRef]
  77. Zhang, K.; Sparling, J.; Chow, B.L.; Elsayed, S.; Hussain, Z.; Church, D.L.; Gregson, D.B.; Louie, T.; Conly, J.M. New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative staphylococci. J. Clin. Microbiol. 2004, 42, 4947–4955. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  78. Schnellmann, C.; Gerber, V.; Rossano, A.; Jaquier, V.; Panchaud, Y.; Doherr, M.G.; Thomann, A.; Straub, R.; Perreten, V. Presence of new mecA and mph(C) variants conferring antibiotic resistance in Staphylococcus spp. isolated from the skin of horses before and after clinic admission. J. Clin. Microbiol. 2006, 44, 4444–4454. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  79. Sutcliffe, J.; Grebe, T.; Tait-Kamradt, A.; Wondrack, L. Detection of erythromycin-resistant determinants by PCR. Antimicrob. Agents Chemother. 1996, 40, 2562–2566. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  80. Gomez-Sanz, E.; Torres, C.; Lozano, C.; Fernandez-Perez, R.; Aspiroz, C.; Ruiz-Larrea, F.; Zarazaga, M. Detection, molecular characterization, and clonal diversity of methicillin-resistant Staphylococcus aureus CC398 and CC97 in Spanish slaughter pigs of different age groups. Foodborne Pathog. Dis. 2010, 7, 1269–1277. [Google Scholar] [CrossRef]
  81. Wondrack, L.; Massa, M.; Yang, B.V.; Sutcliffe, J. Clinical strain of Staphylococcus aureus inactivates and causes efflux of macrolides. Antimicrob. Agents Chemother. 1996, 40, 992–998. [Google Scholar] [CrossRef] [Green Version]
  82. Lina, G.; Quaglia, A.; Reverdy, M.E.; Leclercq, R.; Vandenesch, F.; Etienne, J. Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among staphylococci. Antimicrob. Agents Chemother. 1999, 43, 1062–1066. [Google Scholar] [CrossRef] [Green Version]
  83. Bozdogan, B.; Berrezouga, L.; Kou, M.S.; Yurek, D.A.; Farley, K.A.; Stockman, B.J.; Leclercq, R. A new resistance gene, linB, conferring resistance to lincosamides by nucleotidylation in Enterococcus faecium HM1025. Antimicrob. Agents Chemother. 1999, 43, 925–929. [Google Scholar] [CrossRef] [Green Version]
  84. Lozano, C.; Aspiroz, C.; Rezusta, A.; Gómez-Sanz, E.; Simon, C.; Gómez, P.; Ortega, C.; Revillo, M.J.; Zarazaga, M.; Torres, C. Identification of novel vga(A)-carrying plasmids and a Tn5406-like transposon in meticillin-resistant Staphylococcus aureus and Staphylococcus epidermidis of human and animal origin. Int. J. Antimicrob. Agents 2012, 40, 306–312. [Google Scholar] [CrossRef]
  85. Hammerum, A.M.; Jensen, L.B.; Aarestrup, F.M. Detection of the satA gene and transferability of virginiamycin resistance in Enterococcus faecium from food- animals. FEMS Microbiol. Lett. 1998, 168, 145–151. [Google Scholar] [CrossRef]
  86. Aarestrup, F.M.; Agers, L.Y.; Ahrens, P.; JŁrgensen, J.C.; Madsen, M.; Jensen, L.B. Antimicrobial susceptibility and presence of resistance genes in staphylococci from poultry. Vet. Microbiol. 2000, 74, 353–364. [Google Scholar] [CrossRef]
  87. Van de Klundert, J.A.M.; Vliegenthart, J.S. PCR detection of genes coding for aminoglycoside-modifying enzymes. In Diagnostic Molecular Microbiology: Principles and Applications; Persing, D.H., Smith, T.F., Tenover, F.C., White, T.J., Eds.; American Society for Microbiology: Washington, DC, USA, 1993; pp. 547–552. [Google Scholar]
  88. Kehrenberg, C.; Schwarz, S. Distribution of Florfenicol Resistance Genes fexA and cfr among Chloramphenicol-Resistant Staphylococcus Isolates. Antimicrob. Agents Chemother. 2006, 50, 1156–1163. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  89. Liu, H.; Wang, Y.; Wu, C.; Schwarz, S.; Shen, Z.; Jeon, B.; Ding, S.; Zhang, Q.; Shen, J. A novel phenicol exporter gene, fexB, found in enterococci of animal origin. J. Antimicrob. Chemother. 2012, 67, 322–325. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  90. Mclaws, F.; Chopra, I.; O’Neill, A.J. High prevalence of resistance to fusidic acid in clinical isolates of Staphylococcus epidermidis. J. Antimicrob. Chemother. 2008, 61, 1040–1043. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  91. Chen, H.J.; Hung, W.C.; Tseng, S.P.; Tsai, J.C.; Hsueh, P.R.; Teng, L.J. Fusidic acid resistance determinants in Staphylococcus aureus clinical isolates. Antimicrob. Agents Chemother. 2010, 54, 4985–4991. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Microorganisms 10 00941 g001 550
Figure 1. Minimum spanning tree, based on MLST of 58 S. aureus isolated from farm workers, cows and their surroundings. The minimum spanning tree graph (MST) was created with PHYLOViZ using the goeBURST algorithm. The dominant STs are represented by the circles with larger diameters. Each color represents one sample source. Numbers on lines indicate locus variants between adjacent nodes.
Figure 1. Minimum spanning tree, based on MLST of 58 S. aureus isolated from farm workers, cows and their surroundings. The minimum spanning tree graph (MST) was created with PHYLOViZ using the goeBURST algorithm. The dominant STs are represented by the circles with larger diameters. Each color represents one sample source. Numbers on lines indicate locus variants between adjacent nodes.
Microorganisms 10 00941 g001
Table
Table 1. Antimicrobial resistance of 58 positive isolates of S. aureus.
Table 1. Antimicrobial resistance of 58 positive isolates of S. aureus.
AntibioticsResistantSusceptible
Positive Strains n (%)Positive Strains n (%)
Penicillin (1U)36 (62.1)22 (37.9)
Chloramphenicol (30 μg)2 (3.5)56 (96.5)
Clindamycin (2 μg)2 (3.5)56 (96.5)
Erythromycin (15 μg)4 (6.9)54 (93.1)
Fusidic acid (10 μg)1 (1.7)57 (98.3)
Gentamicin (10 μg)4 (6.9)54 (93.1)
Kanamycin (30 μg)3 (5.2)55 (94.8)
Tetracycline (30 μg)3 (5.2)55 (94.8)
Tobramycin (10 μg)4 (6.9)54 (93.1)
Table
Table 2. S. aureus positive farms, antimicrobial resistance virulence genes, and genetic lineages of the isolates.
Table 2. S. aureus positive farms, antimicrobial resistance virulence genes, and genetic lineages of the isolates.
FarmIsolateSourceMolecular TypingAntimicrobial ResistanceVirulence Factors
ST (CC)spaagrPhenotypeGenotypeIEC
System		Other Genes
3VS3218Cow6 (5)t16615IPENblaZ hla, hlb, hld
6VS3219Human45 (45)t563IPENblaZ hla, hlb, hld
10VS3220Cow6 (5)t16615ISusceptible hla, hlb, hld
VS3221Cow6 (5)t16615IFD hla, hlb, hld
VS3222Cow133 (133)t4735ISusceptible hla, hlb, hld
13VS3223Cow672t959IPEN, CN, TOB, KAN, TETblaZ, aac(6′)-aph(2″), aph(3′)-IIIa, tetK hla, hlb, hld
VS3224Cow6 (5)t16615ISusceptible hla, hlb, hld
VS3225Cow6 (5)t16615IPEN, CN, TOB, KANblaZ, aac(6′)-aph(2″), aph(3′)-IIIa hla, hlb, hld
VS3226Cow6 (5)t16615ITETtetK hla, hlb, hld
VS3227Human30 (30)t012IIIPENblaZ hla, hlb, hld, tst
VS3228Human30 (30)t012IIIPENblaZ hla, hlb, hld, tst
VS3229Human30 (30)t9413IIIPENblaZ hla, hlb, hld
14VS3230Soil6 (5)t16615ISusceptible hla, hlb, hld
15VS3231Cow6 (5)t16615ISusceptible hla, hlb, hld
VS3232Cow6 (5)t16615ISusceptible hla, hlb, hld
16VS3233Cow30 (30)t9413IIIPENblaZ hla, hlb, hld
VS3234Cow30 (30)t9413IIIPENblaZ hla, hlb, hld
VS3235Cow30 (30)t9413IIIPENblaZ hla, hlb, hld
VS3236Human5 (5)t045IIPENblaZ hla, hlb, hld
VS3237Human97 (97)t189ISusceptible hla, hlb, hld
VS3238Human30 (30)t018IIIERYermB hla, hlb, hld, tst
VS3239Human30 (30)t9413IIIPENblaZ hla, hlb, hld
VS3240Human30 (30)t018IIIPENblaZ hla, hlb, hld, tst
VS3241Cow30 (30)t9413IIIPENblaZ hla, hlb, hld
17VS3242Cow133 (133)t7355IPEN, CN, TOB, KANblaZ, aac(6′)-aph(2″), aph(3′)-IIIa, str hla, hlb, hld
20VS3243Human5t002IIPENblaZ hla, hlb, hld
25VS3244Human121 (121)t162IVSusceptible Ehla, hld
39VS3245Cow7464 (30)t871IIISusceptible hla, hlb, hld
VS3246Cow45 (45)t015IPEN Bhla, hld
VS3247Cow352 (97)t267ISusceptible hla, hlb, hld
VS3248Cow7464 (30)t871IIISusceptible hla, hlb, hld
42VS3249Cow2328 (133)t3750IIISusceptible hla, hlb, hld
46VS3250Human45 (45)t350IPENblaZ hla, hlb, hld
47VS3251Cow45 (45)t706IPENblaZBhla, hld
VS3252Human34 (30)t414IIIPENblaZ hla, hlb, hld, tst
48VS3253Human45 (45)t015IPENblaZ hla, hlb, hld
VS3254Human188 (188)t189IPEN, CN, TOBblaZ, aac(6′)-aph(2″) hla, hlb, hld
49VS3255Cow133 (133)t18899ISusceptible hla, hlb, hld
VS3256Cow133 (133)t18899ISusceptible hla, hlb, hld
52VS3257Cow672t959ISusceptible Ghla, hld
VS3258Cow672t959ISusceptible Ghla, hld
VS3259Human8t008IPEN, ERYblaZ, ermC hla, hlb, hld
VS3260Human(30)t012IIIPENblaZ hla, hlb, hld
VS3261Human30 (30)t012IIIPENblaZ hla, hlb, hld
55VS3262Water30 (30)t018IIISusceptible hla, hlb, hld, tst
56VS3263Human545IIPENblaZ hla, hlb, hld
58VS3264Human398t571IPEN, ERY, CD, TETblaZ, ermT, tetK hla, hlb, hld
60VS3265Cow72 (8)t148IPENblaZ hla, hlb, hld
VS3266Cow352 (97)t359IPENblaZ hla, hlb, hld
VS3267Cow72 (8)t148IPENblaZ hla, hlb, hld
62VS3268Cow45 (45)t350IPENblaZ hla, hlb, hld
VS3269Cow45 (45)t7669IPENblaZBhla, hld
VS3270Human(30)t012IIIPEN, ERY, CDblaZ, ermC hla, hlb, hld
VS3271Human45 (45)t7669IPENblaZBhla, hlb, hld
63VS3272Cow133 (133)t18899ISusceptible hla, hlb, hld
VS3273Cow1 (1)t2207IIISusceptible hla, hlb, hld
64VS3274Cow72 (8)t148IPEN, CblaZ, catpC221 hla, hlb, hld
VS3275Human72 (8)t148IPEN, CblaZ, catpC221 hla, hlb, hld
Abbreviations: PEN: Penicillin; CN: gentamycin; TOB: tobramycin; KAN: kanamycin; ERY: erythromycin; CD: clindamycin; TET: tetracycline; C: chloramphenicol; ST: sequence type: CC: clonal complex; IEC: Immune evasion cluster; N.T. not typable.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Silva, V.; Correia, S.; Rocha, J.; Manaia, C.M.; Silva, A.; García-Díez, J.; Pereira, J.E.; Semedo-Lemsaddek, T.; Igrejas, G.; Poeta, P. Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings: A Cross-Sectional Study from the One Health Perspective. Microorganisms 2022, 10, 941. https://doi.org/10.3390/microorganisms10050941

AMA Style

Silva V, Correia S, Rocha J, Manaia CM, Silva A, García-Díez J, Pereira JE, Semedo-Lemsaddek T, Igrejas G, Poeta P. Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings: A Cross-Sectional Study from the One Health Perspective. Microorganisms. 2022; 10(5):941. https://doi.org/10.3390/microorganisms10050941

Chicago/Turabian Style

Silva, Vanessa, Susana Correia, Jaqueline Rocha, Célia M. Manaia, Adriana Silva, Juan García-Díez, José Eduardo Pereira, Teresa Semedo-Lemsaddek, Gilberto Igrejas, and Patrícia Poeta. 2022. "Antimicrobial Resistance and Clonal Lineages of Staphylococcus aureus from Cattle, Their Handlers, and Their Surroundings: A Cross-Sectional Study from the One Health Perspective" Microorganisms 10, no. 5: 941. https://doi.org/10.3390/microorganisms10050941

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop