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Article

Viral and Bacterial Zoonotic Agents in Dromedary Camels from Southern Tunisia: A Seroprevalence Study

by
Simone Eckstein
1,*,
Rosina Ehmann
1,
Abderraouf Gritli
2,
Mohamed Ben Rhaiem
2,
Houcine Ben Yahia
2,
Manuel Diehl
1,
Roman Wölfel
1,
Susann Handrick
1,†,
Mohamed Ben Moussa
3,† and
Kilian Stoecker
1,†
1
Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany
2
Veterinary Service, General Directorate of Military Health, Ministry of National Defense, Tunis 1000, Tunisia
3
Department of Virology, Military Hospital of Instruction of Tunis, Tunis 1008, Tunisia
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2022, 10(4), 727; https://doi.org/10.3390/microorganisms10040727
Submission received: 14 February 2022 / Revised: 17 March 2022 / Accepted: 28 March 2022 / Published: 29 March 2022
(This article belongs to the Special Issue Farm Animal and Wildlife Zoonotic Microorganisms)
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Abstract

:
The rapid spread of SARS-CoV-2 clearly demonstrated the potential of zoonotic diseases to cause severe harm to public health. Having limited access to medical care combined with severe underreporting and a lack of active surveillance, Africa carries a high burden of neglected zoonotic diseases. Therefore, the epidemiological monitoring of pathogen circulation is essential. Recently, we found extensive Middle East respiratory syndrome coronavirus (MERS-CoV) prevalence in free-roaming dromedary camels from southern Tunisia. In this study, we aimed to investigate the seroprevalence, and thus the risk posed to public health, of two additional viral and two bacterial pathogens in Tunisian dromedaries: Rift Valley fever virus (RVFV), foot-and-mouth disease virus (FMDV), Coxiella burnetii and Brucella spp. via ELISA. With 73.6% seropositivity, most animals had previously been exposed to the causative agent of Q fever, C. burnetii. Additionally, 7.4% and 1.0% of the dromedaries had antibodies against Brucella and RVFV, respectively, while no evidence was found for the occurrence of FMDV. Our studies revealed considerable immunological evidence of various pathogens within Tunisian dromedary camels. Since these animals have intense contact with humans, they pose a high risk of transmitting serious zoonotic diseases during active infection. The identification of appropriate countermeasures is therefore highly desirable.

1. Introduction

Disease prevalence and epidemiology data acquisition in Africa is severely hampered by a multitude of factors such as the lack of health care infrastructure or funding. While the One Health concept has fostered many international and collaborative projects to improve knowledge of animal and human diseases in Africa, the overall understanding remains very incomplete for many reasons. To start with, Africa as a continent features a multitude of different geographical and climatic microenvironments even on a national scale. Each of these microenvironments fosters conditions for specific microorganisms, vectors and hosts. Furthermore, such microenvironments are strongly influenced by the different traditions and lifestyles of the ethnicities living in them, for example, huge urban centers, agricultural communities and nomadic herders.
In Tunisia, camel breeding is an important agricultural and economic pillar as animals are used for wool or leather, milk and/or meat production as well as for touristic, transport and patrol purposes [1]. However, camels are affected by various infectious diseases. Hence, direct or indirect contact with camels or the consumption of camel products represents a significant source of zoonotic disease transmission to other domestic animals and humans (Figure 1) [2]. Most African dromedary camels kept for milk or meat production roam freely through the desert most of the year, also crossing international borders. This combined with trafficking increases the risk of unnoticedly introducing pathogens from neighboring countries.
Two of the most important bacterial zoonotic diseases associated with domestic livestock are brucellosis and Q fever, caused by the Gram-negative bacteria Brucella spp. and Coxiella burnetii, respectively. In bovines, small ruminants and camels, an infection with either of these pathogens can lead to abortions, infertility or stillbirth [3,4,5]. Camels are hosts for the two Brucella spp. that predominantly affect domestic livestock fertility and human health: B. abortus and B. melitensis [6]. Infections with both Brucella spp. and C. burnetii are linked to the consumption of raw milk or meat or direct contact with urine, blood, feces or aborted tissues, especially placentas, or the inhalation of contaminated aerosols [7,8]. Additionally, C. burnetii might be also transmitted by ticks (Figure 1) [7]. However, as human brucellosis as well as Q fever lack pathognomonic symptoms but can manifest with fever, headache, skin rash, pneumonia, endocarditis or encephalitis [9,10,11,12,13], infections are often not recognized as such. Moreover, C. burnetii and Brucella spp. have also been deliberately used against humans in the past as part of active bioweapons programs. Therefore, even today, there is a latent risk of the misuse of these pathogens, e.g., by criminals or terrorists. In this context, active epidemiological surveillance in the event of a disease outbreak makes it easier to distinguish between natural origin and possible deliberate release.
Rift Valley fever virus (RVFV), an arthropod-borne phlebovirus of the Phenuiviridae family, is a good example of a pathogen that has to be closely monitored as its geographic distribution is highly influenced by climatic factors and therefore climate change. Mainly transmitted by Aedes and Culex mosquitoes [14,15], RVFV was first reported by a veterinarian in Kenya in the early 1900s and is widespread in sub-Saharan African regions [14], with increasing reports throughout the African continent and Saudi Arabia [16,17,18,19,20]. There are two transmission cycles for RVFV: a low-level enzootic cycle and an epizootic cycle with fulminant outbreaks causing so-called “abortion storms“ in livestock [21,22,23,24] and considerable pathogenicity in humans [25]. Here, clinical symptoms of the disease can include ocular disease, encephalitis or hemorrhagic fever. Besides mosquitos, exposure to contaminated aerosols or body fluids bears the risk of pathogen transmission to humans (Figure 1). Therefore, increased numbers of human RVFV infections are found in agricultural workers, butchers or domestic livestock keepers [26]. Since dromedary camels are susceptible to RVFV, but only have asymptomatic infections [27], they are a potential source of RVFV transmission to humans. Thus, the seroprevalence of this viral zoonotic agent in Tunisian dromedary camels was investigated in this study.
Another viral zoonotic agent causes one of the most feared and regulated livestock diseases worldwide: foot-and-mouth disease (FMD). Foot-and-mouth disease virus (FMDV), genus Aphthovirus, family Picornaviridae [28] can be transmitted either directly or indirectly. While human infections are extremely rare [29], FMD is a highly contagious disease in domestic artiodactyl livestock species and can cause enormous economic losses both for the affected farmers (milk loss or increased mortality) but most importantly on the country level due to national and international trading restrictions [30].
In January 2020, we had the rare opportunity to screen a representative set of 500 dromedary camels in southern Tunisia in mostly nomadic small-scale farming for the prevalence of MERS-CoV [31]. Camel farming in Tunisia and the northern Sahara, in general, consists predominantly of the extensive agricultural type with nomadic herding in the desert using the natural resources and relying on a minimum of extra feed provided [32]. In this pastoral herding setting, animals have more individual space, and therefore, artificial effects caused by the crowding of animals in stables or other closed environments are diminished. This in turn effects disease transmission and prevalence. Human intervention with additional feeding, handling of individual animals for breeding or milking is most intensive during the mating and birthing seasons, which coincides with a high transmission risk of the selected pathogens under study. The samples collected here are therefore highly valuable, as they allow insights into the natural distribution of highly pathogenic and zoonotic microorganisms in free-ranging dromedaries and the risk potential of transmission to the herders in an extensive farming style.

2. Materials and Methods

2.1. Study Design

In this study, sera obtained for an initial MERS-CoV study [31] were re-used to test for additional pathogens. For the previous study, sera were collected from 500 dromedary camels at 20 different sampling sites located around Douz, Mahrouga, Bazma or Ksar Ghilane in the Kebili governorate in southern Tunisia (Figure 2A). At most of the sampling sites (n = 13), the camels were kept for milk and meat production, primarily for subsistence use, and allowed to roam the desert freely throughout the year. From these sites, 382 camels were sampled. While attempts were made to evenly collect samples of each age group within a herd, a truly random sample could not be achieved due to logistical constraints and the nature of the herd structures. Furthermore, extensively farmed dromedary herds consist of one sexually mature male (sultan) and its harem (approximately 20–50 sexually mature females and juvenile offspring of both sexes), resulting in a skew of both gender and age distribution. More female animals (n = 370) than male ones (n = 12) were sampled. Additionally, 118 adult male dromedary camels kept in small, enclosed herds for patrol and transport purposes were sampled. None of the sampled herds were more than 50 km away from the next humid zone, and all of them had regular access to oases.
To collect the sera, camels were caught by the herders and manually restrained. A 4–8 mL blood sample was collected from the jugular vein using an 18-gauge needle into EDTA vacutainer tubes.
The data were analyzed according to the sex (male/female) and age (juvenile: 0–6 months, 6–24 months and adult: 2–6 years, 6–12 years, 12–25 years and >25 years) of the dromedaries as well as sampling location and type of husbandry (free-roaming or enclosed).

2.2. Serological Testing

Seropositivity for Coxiella burnetii (ID Screen®, Q Fever Indirect, IDvet, Grabels, France) as well as Brucella spp. (Anti-Brucella-ELISA Kamel IgG, Euroimmun AG, Luebeck, Germany) was analyzed via an indirect ELISA according to the respective manufacturer’s protocol. Anti-FMDV and anti-RVFV IgG was detected via the competitive ELISA PrioCHECK® FMDV NS (Prionics, Lelystad, The Netherlands) and ID Screen® Rift Valley Fever Competition (IDvet, Grabels, France), respectively, using the protocol provided by the manufacturer. All multi-species ELISA have been shown to be valid for camel sera [33,34,35]. Information on semi-quantitative result interpretation for each ELISA is given in Appendix A Table A1. Borderline results were considered negative for all statistical analyses.

2.3. Statistical Analysis

For statistical analysis, associations between pathogen seroprevalence in dromedary camels and the study parameters (sex, age, sampling site and animal husbandry) were analyzed. Univariable analysis was conducted by either using Fisher’s exact test or Chi-square test (when necessary). Multivariant analysis was calculated by using multiple logistic regression. Calculations were made using the GraphPad Prism software (Version 8, GraphPad Software, San Diego, CA, USA). p-values less than 0.05 were considered statistically significant.

3. Results and Discussion

The zoonotic pathogens analyzed in this study have several reservoir species, especially ruminants. Disease prevalence and epidemiology can vary considerably depending on the composition of the different species kept together in the same area. Due to the costs and challenging infrastructure in the desert with small and widely dispersed free-ranging herds, the large-scale vaccination of camels against zoonotic disease agents is not commonly practiced in Tunisia. Currently, dromedary camels are only vaccinated by default against camelpox virus and Clostridium perfringens, the causative agent of enterotoxemia. It is therefore still highly desirable to attain a broader coverage of seroprevalence studies on the small-scale level to get a better understanding of both the actual distribution and zoonotic impact of microorganisms as well as their biological properties in different microenvironments.

3.1. Majority of Camels Seropositive for Anti-C. burnetii IgG

By screening all 500 dromedary sera for antibodies reactive to C. burnetii, we found that, with 73.6% seropositivity, the majority of the sampled dromedary camels were exposed to the pathogen before (Table 1). The respective IgG antibodies were present in at least one camel serum of every sampling site, with the highest seropositivity ratio in Bazma (Figure 2B).
Univariate analysis revealed that the parameters associated with significantly increased seropositivity were adult age, female gender, free husbandry condition and sampling location. By applying the multivariable analysis, however, only adult age, free roaming and the sampling locations Bazma and Ksar Ghilane remained the significant variables (Table 2). Since we only analyzed seropositivity, thus ignoring active infections with the pathogen, the fact that we found more seropositive adult animals than juveniles is conclusive. Furthermore, the significant influence of the husbandry type on seropositivity could be explained by the fact that the risk of introducing new infections is much lower for camels kept enclosed than for animals that wander through the desert freely, thereby encountering other herds or their excrement and tissue remains.
Coxiellia burnetii is known to be endemic in Tunisia. However, human Q fever cases are rarely described. While C. burnetii has been retrospectively identified as the cause for 9% of hospital admissions [36], only 21 cases of acute Q fever were reported in Tunisia between 2003 and 2007 [37]. Nevertheless, Q fever infections show no pathognomonic symptoms and are therefore often misdiagnosed.
With the exception of one study that reported 44% seropositivity for anti-C. burnetii IgG among 534 camel sera [34], little is known about the distribution of Q fever within Tunisian dromedary camel herds. The results of this study revealed seropositivity ratios almost two-fold as high as described before and hence insinuate the extensive circulation of C. burnetii within dromedary camels in Tunisia. Several serosurveys from other African countries also found high ratios of positive dromedaries, e.g., in Kenya [38], Saudi Arabia [39] and Egypt [40]. However, the role of camels in shedding C. burnetii has not been assessed yet and thus, the question whether dromedaries act as relevant infection reservoirs for humans is a matter of future research.
The extremely low infectious dose of C. burnetii and the fact that the camel keepers regularly consume raw camel milk or assist during the birth of new calves offers many interfaces for the transmission of C. burnetii. Furthermore, the placentas and tissue remnants are usually left untended in the desert. As C. burnetii is a bacterium known for exceptional resistance to environmental stressors and heat compared to other Gram-negative bacteria [41,42], contaminated aerosols could be easily generated and distributed by the constant winds in the desert and inhaled by other livestock or herders. So far, prevalence studies have focused on C. burnetii content in milk and blood. Bacterial transmission, however, most consistently occurs via the genital route—especially during birthing season with contact to placenta or amniotic fluid. Therefore, it would be interesting to gather more information on the amount of C. burnetii shed in vaginal secretions and placentas of camels [43].
In Tunisia, the vaccination of camels against Q fever is uncommon, as the commercially available vaccine is not licensed for the Tunisian market [44]. Anyway, while clinical signs such as fertility problems and abortion could be reduced completely [45] by, e.g., the off-label use of the vaccine, the prevention of the transmission and excretion of the bacterium is not achieved with the currently available vaccines [46,47]. Furthermore, in the camel farming setting in the desert of southern Tunisia, logistics of transporting and administering vaccines to the widely dispersed small herds is extremely difficult. In principle, vaccination in humans is also possible, as there is one vaccine licensed on the market in Australia [48]. However, as the vaccination can lead to severe adverse reactions in individuals with previous contact to C. burnetii, pre-vaccination screening would be required [49]. Thus, other preventative measures such as active surveillance and educational campaigns for the Tunisian population are needed. As even protective materials such as gloves or disinfectants are rare goods in the desert, simple measures such as burying afterbirth remnants in sandpits and heat treatment of camel milk prior to consumption can already considerably reduce the risk of spreading C. burnetii.

3.2. Low Brucella spp. Seropositivity

With over 500,000 new infections annually, brucellosis is among the most common zoonotic diseases worldwide [50,51]. The serological screening for antibodies against Brucella spp. performed in this study revealed 7.4% seropositive animals and therefore no sign of an acute epidemic. However, the 38 seropositive animals provide evidence for the presence of Brucella spp. in northern Africa. Univariate analysis showed that the ratio of seropositivity significantly depended on the sampling location (p = 0.001) with the majority of seropositive animals located in Bazma and Mahrouga (Table 1, Figure 2B). Since these locations are adjacent areas, our findings may be indicative of a previous series of brucellosis in this region. Furthermore, even though all seropositive camels were adult females (Table 1), no significant correlation between seropositivity and the age (p = 0.0647) or sex (p = 0.1151) of the animals could be observed. In addition, the type of husbandry did not have a significant impact on seropositivity (p > 0.9999). Multiple logistic regression analysis was not possible due to the low count of seropositive animals.
In 1991, an outbreak of brucellosis was reported in the southern governorates of Tunisia, particularly in Gafsa, where 407 cases were diagnosed. As the vast majority of patients had previously consumed raw milk, subsequently performed studies on livestock revealed explosive seropositivity among goats and sheep flocks with positivity rates up to 61% [52]. Although such major outbreaks have not recurred in Tunisia, brucellosis is still endemic there. Over the last years, the number of reported human brucellosis cases per 100,000 inhabitants rapidly increased from <2 in 2013 to almost 9 in 2018 [53]. Similar to Q fever, the number of unreported or misdiagnosed infections is probably high due to the lack of pathognomonic symptoms.
As the predominant Brucella species in Tunisia is B. melitensis, a practical countermeasure would be the mass vaccination of livestock using the B. melitensis vaccine strain Rev.1. However, Rev.1 is a live vaccine and induces abortion in nearly all pregnant females [54]. Like cattle, female dromedaries spend nearly their entire reproductive phase pregnant to produce new offspring and yield milk, which excludes the use of live vaccines. Moreover, vaccination is currently restricted to small ruminants and cattle and would therefore require off-label use [52]. Control and prophylactic measures are very hard to conduct in the Tunisian desert, as access to testing and diagnostic facilities is very limited. Therefore, the possibilities to assess the herd status or acquiring Brucella-negative animals are limited. The lack of pathognomonic lesions and symptoms in camels additionally complicates the identification and subsequent elimination of carriers. However, like for C. burnetii, contaminated raw milk and meat products are the most frequent routes of Brucella spp. transmission. Thus, the heating of milk and thorough cooking of meat before consumption can consistently reduce the risk of brucellosis infections and are easy to apply, even in the desert setting [55].

3.3. Sporadic Serologic Evidence of Rift Valley Fever Infections

In this study, only 1% of all camel sera tested positive for antibodies against RVFV, indicating a very low prevalence of the pathogen. Due to the low count of seropositive animals, no statistical analysis was conducted. The five respective seropositive animals were all adult males, kept for transport and patrol purposes at four different sampling sites in Douz (n = 4) and Bazma (n = 1) (Table 1, Figure 2B), hence showing an isolated pattern of seropositivity in dromedaries in Tunisia. Furthermore, not all animals of one artificial herd had antibodies against the virus. This might be explained by the fact that animals of this type of husbandry are often obtained from livestock markets as adults and not bred by the herders themselves like the herds consisting of one sultan and its harem. Hence, it is most likely that the positive males were already exposed to RVFV before reaching the respective sampling sites.
In 2016, North Africa, especially Tunisia, was identified to be a suitable area for the occurrence and spread of the zoonotic disease Rift Valley fever due to its climate conditions [23]. Shortly after, febrile and non-febrile patients seropositive for antibodies against RVFV confirmed the active circulation of the disease in Tunisia [56]. The first serological evidence of RVFV in Tunisian dromedary camels was then reported by Selmi et al. in 2020, who immunologically analyzed sera collected between 2017 and 2018 from dromedaries within six different governorates. Here, 34% of the tested dromedaries were seropositive for anti-RVFV IgG [27]. Moreover, recent findings also demonstrated the prevalence of specific RVFV antibodies in cattle and sheep from different Tunisian areas [57], evidently supporting the circulation of RVFV in Tunisia.
These transmission patterns are influenced by the biology of the transmitting mosquito species Aedes and Culex. Female mosquitos produce infected eggs after taking up RVFV during blood meals on infected reservoir hosts. These eggs are very drought-resistant and are primarily deposited in dry basins that are flooded in times of high precipitation [58]. Flooding subsequently leads to mass-hatching events of young mosquitos and severe outbreak events in animals and humans. Such precipitation events can vary considerably in different geographical locations and microenvironments, leading to the periodic appearance of RFV outbreaks. Enzootic transmission is linked to areas with irrigation that support the low-level reproduction of the vectors of RVFV. Therefore, the low seroprevalence found in this study could be linked to the lack of such suitable biotopes in the desert of southern Tunisia.
Low levels of both precipitation and reservoir host density do not favor sustained virus transmission. Whether the seropositive animals in our study were caused by autochthonous infections linked to enzootic transmission near oases, farms and villages or were merely an effect of animal traffic and acquisition from endemic areas such as Egypt remains unanswered. However, Selmi and colleagues found RVFV seroprevalence levels as high as 61% in camels tested in the governorate of Tozeur, which also has a warm desert climate, and 38% seropositivity in Kebili [27]. This apparent incongruency emphasizes the importance of the concept of microenvironments and supports the need for constant vigilance and surveillance to keep track of RVFV epidemiology.
The containment of RVFV spread in endemic areas such as, e.g., African countries can be achieved by vector control via insecticides and the avoidance of unnecessary accumulations of standing water. For livestock, both inactivated and live-attenuated vaccines are commercially available. The latter type is currently applied for vaccinating camels in endemic countries such as Egypt [59]. In Tunisia, however, livestock is currently not vaccinated against RVFV by default due to the lack of documented clinical importance.

3.4. No Evidence of Foot-and-Mouth Disease

In the present study, no antibodies against FMDV were found in any of the 500 dromedary sera. No vaccination against FMDV was documented for any of the 500 dromedaries, and as immunization is not financially supported by the government, it is unlikely that any of the animals had been vaccinated against FMDV. Therefore, the absence of seropositivity suggests no previous exposure of the animals to the virus. As FMDV is extremely contagious, this is a strong hint that the Kebili governorate is free of FMD. However, Algeria has experienced outbreaks of FMDV [60], and the open borders and transboundary livestock movements is a constant risk of disease introduction.
Despite several vaccination campaigns and other countermeasures, Tunisia had four major episodes of FMD outbreaks between 1989 and 2017. However, as the FMDV serotypes found in Tunisia are mainly associated with foreign countries, the introduction of FMDV was most likely due to illegal trade [61].

3.5. High Serological Evidence for Various Zoonotic Agents in Dromedary Camels of Southern Tunisia

Including the data from our previous MERS-CoV study in the statistics, the majority of dromedaries (87.4%) were seropositive for at least one of the five pathogens (Figure 2C). With 62.6%, more than half of the animals had reactive antibodies against two of the tested zoonotic agents: RVFV + MERS-CoV (0.4%), C. burnetii + MERS-CoV (60.8%), C. burnetii + Brucella (0.2%) and Brucella + MERS-CoV (1.2%). Furthermore, with 5.8% (C. burnetii + Brucella + MERS-CoV) and 0.6% (C. burnetii + RVFV + MERS-CoV), 6.4% of dromedary camels sampled were already exposed to three of the five pathogens tested in our studies. Thus, only 12.6% of camel sera were either seronegative for all five pathogens (10.2%) or within the borderline range (2.4%), emphasizing the immense importance of dromedary camels as reservoirs for zoonotic diseases.

4. Conclusions

The illegal and uncontrolled trade of animals both at national and transboundary levels leads to a high risk of the introduction of zoonotic pathogens. Combined with a lack of hygiene and disease management strategies, this poses the danger of the sustained transmission of these pathogens between Tunisian camels, but also potentially to their keepers.
In this serosurvey, we found a high seroprevalence of anti-C. burnetii IgG in camels from southern Tunisia. Furthermore, we found evidence of Brucella spp. and RVFV circulation, albeit the seropositivity was low. In addition, the same set of samples previously revealed an endemic distribution of MERS-CoV in dromedary camels in this region [31]. In total, almost 90% of the tested animals were seropositive for at least one and up to three of the five pathogens we screened for, hence emphasizing the risk of zoonotic disease transmission to humans by their livestock.
For future surveillance studies, we therefore aim to also screen for active infections to address the limitation of our present study. Furthermore, from a biosecurity perspective, knowledge about geographical distribution and phylogeny of different pathogens is mandatory in order to assess the provenance of a pathogen and the likelihood of a bioterroristic background in the event of a local disease outbreak.
Due to the lack of infrastructure and limited options of livestock vaccination and health screening, reducing the pathogen circulation within the herds poses a difficult task. Hence, raising awareness of the presence and the significance of these microorganisms in the local farming community fosters more hygienic handling practices for both humans and animals, thereby reducing the mutual transmission of pathogens.
Easy-to-conduct measures such as the heat treatment of milk and meat products or hygiene management during the birth season with the removal of potentially contaminated afterbirth tissues could have considerable impacts when practiced routinely.

Author Contributions

S.E., S.H., R.W., M.B.M. and K.S. designed the study. R.E., A.G., H.B.Y., M.D. and M.B.R. collected the dromedary sera in Tunisia; S.E. and M.D. performed the experiments. S.E. analyzed the data and prepared figures and tables. S.E. wrote the original draft. S.E., R.E., R.W., S.H. and K.S. reviewed and edited the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This work is part of Medical Biological Defense Research Program of the Bundeswehr Medical Service and was supported by the Enable and Enhance Initiative of the federal government of Germany (Ertüchtigungsinitiative der Deutschen Bundesregierung, grant no. OR12-370.43 ERT TUN IMB).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

We thank the Deutsche Gesellschaft für Internationale Zusammenarbeit for their support in planning and organizing the study. Furthermore, we gratefully acknowledge the camel keepers in Tunisia for their cooperation and all Tunisian veterinaries and technical assistants of the MHT that supported the mission. Thanks are also due to Maximilian Pankla for technical assistance. Lastly, we thank Daniel Lang for sharing his expertise on statistical analysis of the data.

Conflicts of Interest

The authors declare no conflict of interest. Opinions, interpretations, conclusions and recommendations are those of the authors and are not necessarily endorsed by any governmental agency, department or other institutions. The funders had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Appendix A

Table
Table A1. Summary for semi-quantitative result interpretation for all ELISA kits used in this study. For all assays, extinction (OD) was measured at 450 nm. S = sample; Cal = calibrator; NC = negative control; PC = positive control.
Table A1. Summary for semi-quantitative result interpretation for all ELISA kits used in this study. For all assays, extinction (OD) was measured at 450 nm. S = sample; Cal = calibrator; NC = negative control; PC = positive control.
ELISAResultFormula
NegativeBorderlinePositive
Anti-Brucella ELISA camelRatio < 0.8Ratio ≥ 0.8– < 1.1Ratio ≥ 1.1 Ratio = OD S OD Cal
ID Screen®
Q Fever Indirect		S/P% ≤ 40%S/P% > 40%– ≤ 50%S/P% > 50% S P % = OD sample − OD NC OD PC − OD NC × 100
ID Screen®
RVF Competition		S/N% > 50%S/N% < 40%– ≤ 50%S/N% ≤ 40% S N % = OD sample OD NC × 100
PrioCHECK® FMDV NSPI = < 50%/PI = ≥ 50% PI = 100 − ( OD sample OD NC ) × 100

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Microorganisms 10 00727 g001 550
Figure 1. Schematic overview of pathogen transmission pathways. Rift Valley fever virus (RVFV, green), Foot-and-mouth disease virus (FMDV, purple), Brucella spp. (pink) and Coxiella burnetii (yellow) can be transmitted from dromedary camels both directly and indirectly. Furthermore, RVFV and C. burnetii can be transmitted by mosquitos and tick bites, respectively.
Figure 1. Schematic overview of pathogen transmission pathways. Rift Valley fever virus (RVFV, green), Foot-and-mouth disease virus (FMDV, purple), Brucella spp. (pink) and Coxiella burnetii (yellow) can be transmitted from dromedary camels both directly and indirectly. Furthermore, RVFV and C. burnetii can be transmitted by mosquitos and tick bites, respectively.
Microorganisms 10 00727 g001
Microorganisms 10 00727 g002 550
Figure 2. Sampling locations and results of serological screening. (A) Map of Tunisia with its different governorates. The four main sampling sites in Kebili (brown): Bazma, Mahrouga, Douz and Ksar Ghilane are highlighted with red dots. (B) Seropositivity of animals for the different pathogens sorted by sampling area. (C) Venn diagram depicting the ELISA results. Yellow = Coxiella burnetii; pink = Brucella spp.; green = RVFV; blue = MERS-CoV; purple = FMDV; light gray = borderline; dark gray = negative. Dotted line: 50%. The numbers for MERS-CoV seroprevalence were adapted from our previous study [31].
Figure 2. Sampling locations and results of serological screening. (A) Map of Tunisia with its different governorates. The four main sampling sites in Kebili (brown): Bazma, Mahrouga, Douz and Ksar Ghilane are highlighted with red dots. (B) Seropositivity of animals for the different pathogens sorted by sampling area. (C) Venn diagram depicting the ELISA results. Yellow = Coxiella burnetii; pink = Brucella spp.; green = RVFV; blue = MERS-CoV; purple = FMDV; light gray = borderline; dark gray = negative. Dotted line: 50%. The numbers for MERS-CoV seroprevalence were adapted from our previous study [31].
Microorganisms 10 00727 g002
Table
Table 1. Seropositive dromedary camels by zoonotic diseases and different sampling parameters. Gray shaded lines: Male animals kept enclosed for transport and patrol purposes.
Table 1. Seropositive dromedary camels by zoonotic diseases and different sampling parameters. Gray shaded lines: Male animals kept enclosed for transport and patrol purposes.
Sampling
Parameter		No.
Dromedaries		No. Seropositive Dromedaries (%)
Coxiella burnetiiBrucella spp.Rift Valley Fever Virus
500368 (73.6)37 (7.4)5 (1.0)
Sex
Male13068 (52.3)5 (3.8)5 (3.8)
Female370300 (81.1)32 (8.6)0
Age
Juvenile4513 (28.9)00
0–6 months4000
6–24 months4113 (31.7)00
Adult455355 (78.0)37 (8.1)5 (1.1)
2–6 years8040 (50.0)4 (5.0)0
6–12 years179142 (79.3)18 (10.1)4 (2.2)
12–25 years190167 (87.9)14 (7.4)1 (0.5)
>25 years66 (100)1 (16.7)0
Sampling locations
Ksar Ghilane (∑ = 6)211144 (68.2)5 (2.4)0
Site 1289 (32.1)00
Site 2207 (35.0)2 (10.0)0
Site 33022 (73.3)00
Site 42019 (95.0)00
Site 57354 (74.0)3 (4.1)0
Site 64033 (82.5)00
Bazma (∑ = 7)167143 (85.6)20 (12.0)2 (1.2)
Site 12521 (84.0)00
Site 23028 (93.3)2 (6.7)0
Site 31514 (93.3)5 (33.3)0
Site 41515 (100)3 (20.0)0
Site 52118 (85.7)2 (9.5)0
Site 61614 (87.5)4 (25.0)0
Site 74533 (73.3)4 (8.9)2 (4.4)
Douz (∑ = 5)5322 (41.5)3 (5.7)3 (5.7)
Site 1a43 (75.0)01 (25.0)
Site 1b41 (25.0)00
Site 22410 (41.7)2 (8.3)1 (4.2)
Site 3187 (38.9)1 (5.6)1 (5.6)
Site 431 (33.3)00
Mahrouga (∑ = 2)6958 (84.1)9 (13.0)0
Site 14235 (83.3)5 (11.9)0
Site 22723 (85.2)4 (14.8)0
Animal husbandry
Free-roaming382306 (80.1)28 (7.3)0
Kept enclosed11862 (52.5)9 (7.6)5 (4.2)
Bold and italicized headings describe the superior category of the tested variables. Bold subheading describe the respective subordinate categories.
Table
Table 2. Univariate and multivariate analysis of anti-C. burnetii IgG seropositivity. Borderline results were considered negative for statistical analyses.
Table 2. Univariate and multivariate analysis of anti-C. burnetii IgG seropositivity. Borderline results were considered negative for statistical analyses.
VariableUnivariate Multivariate c
OR (95% CI)p ValueOR (95% CI)p Value
Sex
Female3.91 (2.5–5.94)<0.0001 (****) a0.7846 (0.21–2.50)0.6962 (ns)
Age
Adult (>2 y)8.74 (4.50–17.71)<0.0001 (****) a16.06 (7.49–36.92)<0.0001 (****)
Husbandry
Free roaming3.64 (2.356–5.60)<0.0001 (****) a14.4 (3.85–61.77)0.0001 (***)
Location Site <0.0001 (****) b
Ksar Ghilane 0.35 (0.20–0.61)0.0002 (***)
Bazma 3.62 (2.10–6.46)<0.0001 (****)
Douz 0.54 (0.25–1.14)0.1067 (ns)
Mahrouga 0.93 (0.44–2.10)0.8481 (ns)
a Fisher’s exact test; b Chi-square test; c multiple logistic regression. Bold and italicized headings describe the superior category of the tested variables. **** p < 0.0001; *** p ≤ 0.001; ns not significant (p > 0.05)
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Eckstein, S.; Ehmann, R.; Gritli, A.; Ben Rhaiem, M.; Ben Yahia, H.; Diehl, M.; Wölfel, R.; Handrick, S.; Ben Moussa, M.; Stoecker, K. Viral and Bacterial Zoonotic Agents in Dromedary Camels from Southern Tunisia: A Seroprevalence Study. Microorganisms 2022, 10, 727. https://doi.org/10.3390/microorganisms10040727

AMA Style

Eckstein S, Ehmann R, Gritli A, Ben Rhaiem M, Ben Yahia H, Diehl M, Wölfel R, Handrick S, Ben Moussa M, Stoecker K. Viral and Bacterial Zoonotic Agents in Dromedary Camels from Southern Tunisia: A Seroprevalence Study. Microorganisms. 2022; 10(4):727. https://doi.org/10.3390/microorganisms10040727

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Eckstein, Simone, Rosina Ehmann, Abderraouf Gritli, Mohamed Ben Rhaiem, Houcine Ben Yahia, Manuel Diehl, Roman Wölfel, Susann Handrick, Mohamed Ben Moussa, and Kilian Stoecker. 2022. "Viral and Bacterial Zoonotic Agents in Dromedary Camels from Southern Tunisia: A Seroprevalence Study" Microorganisms 10, no. 4: 727. https://doi.org/10.3390/microorganisms10040727

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