Performance of MALDI-TOF Mass Spectrometry (VITEK MS) in the Identification of Salmonella Species
Abstract
:1. Introduction
2. Materials and Methods
2.1. Clinical Isolates
2.2. Final Identification by Serotyping
2.3. Identification by VITEK MS and VITEK2 Systems
2.4. Database and Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Najjar, Z.; Furlong, C.; Stephens, N.; Shadbolt, C.; Maywood, P.; Conaty, S.; Hogg, G. An outbreak of Salmonella Infantis gastroenteritis in a residential aged care facility associated with thickened fluids. Epidemiol. Infect. 2012, 140, 2264–2272. [Google Scholar] [CrossRef] [PubMed]
- Alzwghaibi, A.B.; Yahyaraeyat, R.; Fasaei, B.N.; Langeroudi, A.G.; Salehi, T.Z. Rapid molecular identification and differentiation of common Salmonella serovars isolated from poultry, domestic animals and foodstuff using multiplex PCR assay. Arch. Microbiol. 2018, 200, 1009–1016. [Google Scholar] [CrossRef] [PubMed]
- Reis, R.O.D.; Souza, M.N.; Cecconi, M.C.P.; Timm, L.; Ikuta, N.; Simon, D.; Wolf, J.M.; Lunge, V.R. Increasing prevalence and dissemination of invasive nontyphoidal Salmonella serotype Typhimurium with multidrug resistance in hospitalized patients from southern Brazil. Braz. J. Infect. Dis. 2018, 22, 424–432. [Google Scholar] [CrossRef] [PubMed]
- Deng, J.K.; Fu, L.; Wang, R.L.; Yu, N.; Ding, X.X.; Jiang, L.X.; Fang, Y.P.; Jiang, C.H.; Lin, L.J.; Wang, Y.; et al. Comparison of MALDI-TOF MS, gene sequencing and the Vitek 2 for identification of seventy-three clinical isolates of enteropathogens. J. Thorac. Dis. 2014, 6, 539–544. [Google Scholar] [CrossRef] [PubMed]
- Tsuchida, S.; Nakayama, T. MALDI-Based Mass Spectrometry in Clinical Testing: Focus on Bacterial Identification. Appl. Sci. 2022, 12, 2814. [Google Scholar] [CrossRef]
- Richter, S.S.; Sercia, L.; Branda, J.A.; Burnham, C.A.; Bythrow, M.; Ferraro, M.J.; Garner, O.B.; Ginocchio, C.C.; Jennemann, R.; Lewinski, M.A.; et al. Identification of Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the VITEK MS system. Eur. J. Clin. Microbiol. Infect. Dis. 2013, 32, 1571–1578. [Google Scholar] [CrossRef] [PubMed]
- Lee, M.; Chung, H.S.; Moon, H.W.; Lee, S.H.; Lee, K. Comparative evaluation of two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems, Vitek MS and Microflex LT, for the identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. J. Microbiol. Methods 2015, 113, 13–15. [Google Scholar] [CrossRef] [PubMed]
- Lee, W.; Kim, M.; Yong, D.; Jeong, S.H.; Lee, K.; Chong, Y. Evaluation of VITEK mass spectrometry (MS), a matrix-assisted laser desorption ionization time-of-flight MS system for identification of anaerobic bacteria. Ann. Lab. Med. 2015, 35, 69–75. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Kim, S.Y.; Park, J.S.; Hong, Y.J.; Kim, T.S.; Hong, K.; Song, K.H.; Lee, H.; Kim, E.S.; Kim, H.B.; Park, K.U.; et al. Microarray-Based Nucleic Acid Assay and MALDI-TOF MS Analysis for the Detection of Gram-Negative Bacteria in Direct Blood Cultures. Am. J. Clin. Pathol. 2019, 151, 143–153. [Google Scholar] [CrossRef] [PubMed]
- Shin, J.H.; Kim, S.H.; Lee, D.; Lee, S.Y.; Chun, S.; Lee, J.H.; Won, E.J.; Choi, H.J.; Choi, H.W.; Kee, S.J.; et al. Performance Evaluation of VITEK MS for the Identification of a Wide Spectrum of Clinically Relevant Filamentous Fungi Using a Korean Collection. Ann. Lab. Med. 2021, 41, 214–220. [Google Scholar] [CrossRef] [PubMed]
- Dieckmann, R.; Malorny, B. Rapid screening of epidemiologically important Salmonella enterica subsp. enterica serovars by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry. Appl. Environ. Microbiol. 2011, 77, 4136–4146. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Sparbier, K.; Weller, U.; Boogen, C.; Kostrzewa, M. Rapid detection of Salmonella sp. by means of a combination of selective enrichment broth and MALDI-TOF MS. Eur. J. Clin. Microbiol. Infect. Dis. 2012, 31, 767–773. [Google Scholar] [CrossRef] [PubMed]
- Fukuyama, Y.; Ojima-Kato, T.; Nagai, S.; Shima, K.; Funatsu, S.; Yamada, Y.; Tamura, H.; Nomura, S.; Ogata, K.; Sekiya, S.; et al. Improved MALDI-MS method for the highly sensitive and reproducible detection of biomarker peaks for the proteotyping of Salmonella serotypes. J. Mass Spectrom. 2019, 54, 966–975. [Google Scholar] [CrossRef] [PubMed]
- Kim, S.H.; Park, E.H.; Hwang, I.Y.; Lee, H.M.; Song, A.M.; Lee, M.A. Serotyping and Antimicrobial Susceptibility of Salmonella Isolated in Korea in 2015. Ann. Lab. Med. 2019, 22, 55–60. [Google Scholar] [CrossRef]
- Kim, S.H.; Sung, G.H.; Park, E.H.; Hwang, I.Y.; Kim, G.R.; Song, S.A.; Lee, H.K.; Uh, Y.; Kim, Y.A.; Jeong, S.H.; et al. Serotype Distribution and Antimicrobial Resistance of Salmonella Isolates in Korea between 2016 and 2017. Ann. Lab. Med. 2022, 42, 268–273. [Google Scholar] [CrossRef] [PubMed]
- Ling, T.K.; Liu, Z.K.; Cheng, A.F. Evaluation of the VITEK 2 system for rapid direct identification and susceptibility testing of gram-negative bacilli from positive blood cultures. J. Clin. Microbiol. 2003, 41, 4705–4707. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Guo, L.; Ye, L.; Zhao, Q.; Ma, Y.; Yang, J.; Luo, Y. Comparative study of MALDI-TOF MS and VITEK 2 in bacteria identification. J. Thorac. Dis. 2014, 6, 534–538. [Google Scholar] [CrossRef] [PubMed]
- Wattal, C.; Oberoi, J.K.; Goel, N.; Raveendran, R.; Khanna, S. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for rapid identification of micro-organisms in the routine clinical microbiology laboratory. Eur. J. Clin. Microbiol. Infect. Dis. 2017, 36, 807–812. [Google Scholar] [CrossRef] [PubMed]
- Bastin, B.; Bird, P.; Benzinger, M.J.; Crowley, E.; Agin, J.; Goins, D.; Sohier, D.; Timke, M.; Shi, G.; Kostrzewa, M. Confirmation and Identification of Salmonella spp., Cronobacter spp., and Other Gram-Negative Organisms by the Bruker MALDI Biotyper Method: Collaborative Study Method Extension to Include Campylobacter Species, Revised First Action 2017.09. J. AOAC Int. 2019, 102, 1595–1616. [Google Scholar] [CrossRef] [PubMed]
System Type | Database List |
---|---|
VITEK2 system | Salmonella group |
Salmonella ser. Gallinarum | |
Salmonella ser. Paratyphi A | |
Salmonella ser. Typhi | |
Salmonella enterica ssp. arizonae | |
Salmonella enterica ssp. diarizonae | |
VITEK MS v3.0 | Salmonella group |
Salmonella ser. Gallinarum | |
Salmonella ser. Paratyphi A | |
Salmonella ser. Typhi | |
Salmonella enterica ssp. arizonae/diarizonae | |
VITEK MS v3.2 | Salmonella enterica ssp. enterica |
Salmonella enterica ssp. arizonae/diarizonae |
Serotype | VITEK MS v3.0 | VITEK MS v3.2 |
---|---|---|
(n = 1167) | (n = 222) | |
N | N | |
I4,[5],12:i:- | 220 | 38 |
Enteritidis | 171 | 65 |
Bareilly | 121 | 21 |
Typhimurium | 87 | 10 |
Infantis | 83 | 22 |
Thompson | 56 | 1 |
Agona | 53 | 6 |
Typhi | 51 | 13 |
Montevideo | 34 | 4 |
Livingstone | 34 | 0 |
Stanley | 26 | 1 |
Virchow | 20 | 2 |
Panama | 20 | 2 |
Newport | 16 | 6 |
Saintpaul | 15 | 2 |
Paratyphi A | 14 | 6 |
Mbandaka | 14 | 1 |
Braenderup | 11 | 4 |
Othmarschen | 10 | 3 |
Rissen | 9 | 3 |
Paratyphi B | 7 | 2 |
Others † | 95 | 10 |
VITEK MS (N) | MALDI-TOF VITEK MS Results | n (%) |
---|---|---|
v3.0 (1167) | Salmonella group | 1157 (99.1) |
Salmonella ser. Paratyphi A | 3 (0.3) | |
Salmonella ser. Paratyphi A/Salmonella group | 3 (0.3) | |
Salmonella ser. Typhi/Salmonella group | 3 (0.3) | |
Salmonella enterica ssp. arizonae/Salmonella enterica ssp. diarizonae | 1 (0.1) | |
v3.2 (222) | Salmonella enterica ssp. enterica | 221 (99.5) |
Salmonella enterica ssp. enterica/Salmonella enterica ssp. arizonae/Salmonella enterica ssp. diarizonae | 1 (0.5) |
VITEK MS | Serotype (n) | N | VITEK MSs | VITEK2 System |
---|---|---|---|---|
v3.0 | Paratyphi A (14) | 8 | Salmonella group | Salmonella ser. Paratyphi A |
3 | Salmonella ser. Paratyphi A | Salmonella ser. Paratyphi A | ||
3 | Salmonella ser. Paratyphi A / Salmonella group | Salmonella ser. Paratyphi A | ||
Paratyphi B (7) | 7 | Salmonella group | Salmonella group | |
Typhi (51) | 48 | Salmonella group | Salmonella ser. Typhi | |
3 | Salmonella ser. Typhi Salmonella group | Salmonella ser. Typhi | ||
IIIb 47:r:z (1) | 1 | Salmonella group | Salmonella enterica ssp. diarizonae | |
IIIb 48:k:z (1) | 1 | Salmonella enterica ssp. arizonae/Salmonella enterica ssp. diarizonae | Salmonella enterica ssp. diarizonae | |
v3.2 | Paratyphi A (6) | 6 | Salmonella enterica ssp. enterica | Salmonella ser. Paratyphi A |
Paratyphi B (2) | 2 | Salmonella enterica ssp. enterica | Salmonella group | |
Typhi (13) | 13 | Salmonella enterica ssp. enterica | Salmonella ser. Typhi | |
IIIb 47:r:z (1) | 1 | Salmonella enterica ssp. enterica | Salmonella enterica ssp. diarizonae | |
IIIb 48:k:z (2) | 1 | Salmonella enterica ssp. enterica | Salmonella enterica ssp. diarizonae | |
1 | Salmonella enterica ssp. enterica/Salmonella enterica ssp. arizonae/Salmonella enterica ssp. diarizonae | Salmonella enterica ssp. diarizonae |
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Kim, G.R.; Kim, S.H.; Kim, E.-Y.; Park, E.H.; Hwang, I.Y.; Jeong, S.H.; Kim, H.S.; Kim, Y.A.; Uh, Y.; Shin, K.S.; et al. Performance of MALDI-TOF Mass Spectrometry (VITEK MS) in the Identification of Salmonella Species. Microorganisms 2022, 10, 1974. https://doi.org/10.3390/microorganisms10101974
Kim GR, Kim SH, Kim E-Y, Park EH, Hwang IY, Jeong SH, Kim HS, Kim YA, Uh Y, Shin KS, et al. Performance of MALDI-TOF Mass Spectrometry (VITEK MS) in the Identification of Salmonella Species. Microorganisms. 2022; 10(10):1974. https://doi.org/10.3390/microorganisms10101974
Chicago/Turabian StyleKim, Gyu Ri, Si Hyun Kim, Eun-Young Kim, Eun Hee Park, In Yeong Hwang, Seok Hoon Jeong, Hyun Soo Kim, Young Ah Kim, Young Uh, Kyeong Seob Shin, and et al. 2022. "Performance of MALDI-TOF Mass Spectrometry (VITEK MS) in the Identification of Salmonella Species" Microorganisms 10, no. 10: 1974. https://doi.org/10.3390/microorganisms10101974
APA StyleKim, G. R., Kim, S. H., Kim, E.-Y., Park, E. H., Hwang, I. Y., Jeong, S. H., Kim, H. S., Kim, Y. A., Uh, Y., Shin, K. S., Kim, Y. R., Ryoo, N., Shin, J. H., & Shin, J. H. (2022). Performance of MALDI-TOF Mass Spectrometry (VITEK MS) in the Identification of Salmonella Species. Microorganisms, 10(10), 1974. https://doi.org/10.3390/microorganisms10101974