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Article

Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material

1
Department of Urology, University of California Los Angeles, Los Angeles, CA 90024, USA
2
Vancouver Prostate Centre, Department of Urologic Sciences, University of British Columbia, Vancouver, BC V6H 3Z6, Canada
3
The Stone Centre at Vancouver General Hospital, Department of Urologic Sciences, University of British Columbia, Vancouver, BC V6H 3Z6, Canada
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(9), 764; https://doi.org/10.3390/pathogens9090764
Received: 2 June 2020 / Revised: 3 September 2020 / Accepted: 15 September 2020 / Published: 18 September 2020
(This article belongs to the Section Human Pathogens)
Despite routine implementation in urology, indwelling ureteral stents pose as a nidus for infection. Conditioning film accumulates on stents, which prime pathogen adhesion, promoting infectious biofilm formation. However, the extent to which conditioning film components play a role in facilitating bacterial adhesion and biofilm formation remains largely unknown. Here, we examined the interaction of previously identified stent-bound conditioning film components (fibrinogen, uromodulin, and albumin) with bacterial uropathogens. Cytoscopically removed stents were incubated with common uropathogens (Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus). Immunofluorescent double staining was performed to study the localization of uropathogens relative to stent-bound conditioning film proteins. Conditioning film components were identified on the external stent surface with some deposition in the inner lumen. Bacteria co-localized with fibrinogen, uromodulin, and albumin, suggesting a potential mechanism for stent-associated infections. Here, we determine strong co-localization between common uropathogenic bacterial species with prominent conditioning film components on ureteral stents. Further functional validation of interactions amongst these uropathogens and conditioning film proteins may enhance clinical management for stent-associated infections and development of improved stent technologies. View Full-Text
Keywords: urinary tract infection; ureteral stent; biofilm urinary tract infection; ureteral stent; biofilm
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MDPI and ACS Style

Scotland, K.B.; Kung, S.H.; Chew, B.H.; Lange, D. Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material. Pathogens 2020, 9, 764. https://doi.org/10.3390/pathogens9090764

AMA Style

Scotland KB, Kung SH, Chew BH, Lange D. Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material. Pathogens. 2020; 9(9):764. https://doi.org/10.3390/pathogens9090764

Chicago/Turabian Style

Scotland, Kymora B., Sonia H. Kung, Ben H. Chew, and Dirk Lange. 2020. "Uropathogens Preferrentially Interact with Conditioning Film Components on the Surface of Indwelling Ureteral Stents Rather than Stent Material" Pathogens 9, no. 9: 764. https://doi.org/10.3390/pathogens9090764

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