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Open AccessCommunication

Identification of lncRNAs Involved in PCV2 Infection of PK-15 Cells

1
College of Animal Sciences, Zhejiang University, Hangzhou 310058, China
2
Henan Provincial Engineering and Technology Center of Animal Disease Diagnosis and Integrated Control, Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, 1638 Wolong Road, Wolong District, Nanyang 473061, China
3
Guangdong Provincial Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510640, China
*
Author to whom correspondence should be addressed.
These authors contribute equally to the work.
Pathogens 2020, 9(6), 479; https://doi.org/10.3390/pathogens9060479
Received: 8 May 2020 / Revised: 12 June 2020 / Accepted: 16 June 2020 / Published: 17 June 2020
(This article belongs to the Section Animal Pathogens)
Porcine circovirus type 2 (PCV2) can cause severe disease in infected pigs, resulting in massive economic loss for the swine industry. Transcriptomic and proteomic approaches have been widely employed to identify the underlying molecular mechanisms of the PCV2 infection. Numerous differentially expressed mRNAs, miRNAs, and proteins, together with their associated signaling pathways, have been identified during PCV2 infection, paving the way for analysis of their biological functions. Long noncoding RNAs (lncRNAs) are important regulators of multiple biological processes. However, little is known regarding their role in the PCV2 infection. Hence, in our study, RNA-seq was performed by infecting PK-15 cells with PCV2. Analysis of the differentially expressed genes (DEGs) suggested that the cytoskeleton, apoptosis, cell division, and protein phosphorylation were significantly disturbed. Then, using stringent parameters, six lncRNAs were identified. Additionally, potential targets of the lncRNAs were predicted using both cis- and trans-prediction methods. Interestingly, we found that the HOXB (Homeobox B) gene cluster was probably the target of the lncRNA LOC106505099. Enrichment analysis of the target genes showed that numerous developmental processes were altered during PCV2 infection. Therefore, our study revealed that lncRNAs might affect porcine embryonic development through the regulation of the HOXB genes. View Full-Text
Keywords: PCV2; lncRNA; pig; RNA-seq PCV2; lncRNA; pig; RNA-seq
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MDPI and ACS Style

He, J.; Leng, C.; Pan, J.; Li, A.; Zhang, H.; Cong, F.; Wang, H. Identification of lncRNAs Involved in PCV2 Infection of PK-15 Cells. Pathogens 2020, 9, 479.

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