Next Article in Journal
Inhibition of Asaia in Adult Mosquitoes Causes Male-Specific Mortality and Diverse Transcriptome Changes
Previous Article in Journal
Organic Amendments Modulate Soil Microbiota and Reduce Virus Disease Incidence in the TSWV-Tomato Pathosystem

This is an early access version, the complete PDF, HTML, and XML versions will be available soon.

Open AccessArticle

Identification of Haemaphysalis longicornis Genes Differentially Expressed in Response to Babesia microti Infection

1
The Collaboration Unit for Field Epidemiology of State Key Laboratory for Infectious Disease Prevention and Control, Jiangxi Provincial Key Laboratory of Animal-Origin and Vector-Borne Diseases, Nanchang Center for Disease Control and Prevention, Honggutan New District, Nanchang, Jiangxi 330038, China
2
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, Japan
*
Authors to whom correspondence should be addressed.
Pathogens 2020, 9(5), 378; https://doi.org/10.3390/pathogens9050378
Received: 14 April 2020 / Revised: 2 May 2020 / Accepted: 12 May 2020 / Published: 14 May 2020
(This article belongs to the Section Human Pathogens)
Haemaphysalis longicornis is a tick and a vector of various pathogens, including the human pathogenetic Babesia microti. The objective of this study was to identify female H. longicornis genes differentially expressed in response to infection with B. microti Gray strain by using a suppression subtractive hybridization (SSH) procedure. A total of 302 randomly selected clones were sequenced and analyzed in the forward subtracted SSH cDNA library related to Babesia infection, and 110 clones in the reverse cDNA library. Gene ontology assignments and sequence analyses of tick sequences in the forward cDNA library showed that 14 genes were related to response to stimulus or/and immune system process, and 7 genes had the higher number of standardized sequences per kilobase (SPK). Subsequent real-time PCR detection showed that eight genes including those encoding for Obg-like ATPase 1 (ola1), Calreticulin (crt), vitellogenin 1 (Vg1) and Vg2 were up-regulated in fed ticks. Compared to uninfected ticks, infected ticks had six up-regulated genes, including ola1, crt and Vg2. Functional analysis of up-regulated genes in fed or Babesia-infected ticks by RNA interference showed that knockdown of crt and Vg2 in infected ticks and knockdown of ola1 in uninfected ticks accelerated engorgement. In contrast, Vg1 knockdown in infected ticks had delayed engorgement. Knockdown of crt and Vg1 in infected ticks decreased engorged female weight. Vg2 knockdown reduced B. microti infection levels by 51% when compared with controls. The results reported here increase our understanding of roles of H. longicornis genes in blood feeding and B. microti infection.
Keywords: Haemaphysalis longicornis; Babesia microti; vector-pathogen interactions; RNA interference; vaccine Haemaphysalis longicornis; Babesia microti; vector-pathogen interactions; RNA interference; vaccine
MDPI and ACS Style

Zheng, W.; Umemiya-Shirafuji, R.; Chen, S.; Okado, K.; Adjou Moumouni, P.F.; Suzuki, H.; Yang, S.; Liu, M.; Xuan, X. Identification of Haemaphysalis longicornis Genes Differentially Expressed in Response to Babesia microti Infection. Pathogens 2020, 9, 378.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop