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Genomic Comparison of Salmonella Enteritidis Strains Isolated from Laying Hens and Humans in the Abruzzi Region during 2018

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Bacteriology and Diary Production Hygiene department, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’, Campo Boario, 64100 Teramo, Italy
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Food Microbiology department, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Via Appia Nuova 1411, 00178 Rome, Italy
3
Epidemiology and Risk Analysis department, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’, Campo Boario, 64100 Teramo, Italy
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(5), 349; https://doi.org/10.3390/pathogens9050349
Received: 31 March 2020 / Revised: 29 April 2020 / Accepted: 1 May 2020 / Published: 5 May 2020
Salmonellosis is a major cause of bacterial foodborne infection. Since 2016, an increased number of cases of gastroenteritis caused by Salmonella enterica serovar Enteritidis linked to eggs produced in Poland has been reported in Europe. In Italy, S. Enteritidis is one of the three most commonly reported serotypes, associated mainly with the consumption of contaminated eggs and derived products. In our work, we analysed 61 strains of S. Enteritidis obtained from humans and farms in the Abruzzi region, Italy, in 2018. We used Multiple-Loci Variable-Number Tandem Repeat (VNTR) analysis (MLVA)-based typing and Whole-Genome Sequencing (WGS) tools to identify closely related strains and perform cluster analysis. We found two clusters of genetically similar strains. The first one was present in the local farms and isolated from human cases and had single-linkage distance of no more than two core genes and less than five Single-Nucleotide Polymorphisms (SNPs). The second cluster contained strains isolated from humans and from a dessert (tiramisù) sample that shared identical core genome and were assigned the same SNP address. Cluster 2 isolates were found to be genetically similar to an S. Enteritidis strain from a multi-country outbreak linked to Polish eggs. View Full-Text
Keywords: Salmonella Enteritidis; salmonellosis; cgMLST; SNP address; epidemiology Salmonella Enteritidis; salmonellosis; cgMLST; SNP address; epidemiology
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Di Marcantonio, L.; Janowicz, A.; Zilli, K.; Romantini, R.; Bilei, S.; Paganico, D.; Persiani, T.; Di Donato, G.; Di Giannatale, E. Genomic Comparison of Salmonella Enteritidis Strains Isolated from Laying Hens and Humans in the Abruzzi Region during 2018. Pathogens 2020, 9, 349.

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