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Open AccessArticle

Innovative Alternatives for Continuous In Vitro Culture of Babesia bigemina in Medium Free of Components of Animal Origin

1
Babesia Unit-CENID-Salud Animal e Inocuidad, INIFAP, Carr. Fed. Cuernavaca-Cuautla No. 8534, Col. Progreso, Jiutepec, Morelos C.P. 62550, Mexico
2
Animal Disease Research Unit, Agricultural Research Service, US Department of Agriculture, Pullman, WA 99164-7040, USA
*
Authors to whom correspondence should be addressed.
Pathogens 2020, 9(5), 343; https://doi.org/10.3390/pathogens9050343
Received: 4 April 2020 / Revised: 29 April 2020 / Accepted: 29 April 2020 / Published: 1 May 2020
(This article belongs to the Special Issue Animal Parasitic Diseases)
In this study, we report Babesia bigemina proliferation in culture medium free of components of animal origin supplemented with a lipid mixture. Babesia bigemina continuously proliferated in VP-SFM with a higher percent parasitized erythrocyte as compare to using other animal component-free culture media. Compared with Advanced DMEM/F12 (ADMEM/F12), VP-SFM had a similar percent parasitized erythrocyte (PPE). Supplementation of VP-SF with a lipid acid mixture improved B. bigemina proliferation in vitro culture, with a maximum PPE of 11.3%. Growth of B. bigemina in a perfusion bioreactor using VP-SFM medium supplemented with lipid mixture resulted in a PPE above 28%. In conclusion, we demonstrated that B. bigemina proliferated in an animal component-free medium supplemented with the fatty acid mixture. This innovation to B. bigemina in vitro culture method presented herein is an important source of biological material for live vaccine production and understanding the mechanisms and molecules involved in parasite attachment and invasion of bovine erythrocytes. View Full-Text
Keywords: Babesia bigemina; in vitro culture; animal component-free medium; CD-lipids Babesia bigemina; in vitro culture; animal component-free medium; CD-lipids
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Álvarez Martínez, J.A.; Figueroa Millán, J.V.; Ueti, M.W.; Rojas-Martínez, C. Innovative Alternatives for Continuous In Vitro Culture of Babesia bigemina in Medium Free of Components of Animal Origin. Pathogens 2020, 9, 343.

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