Next Article in Journal
Sustainable and Ecofriendly Approach of Managing Soil Born Bacterium Ralstonia solanacearum (Smith) Using Dried Powder of Conyza canadensis
Previous Article in Journal
Transcriptional Alteration of Gene Biomarkers in Hemocytes of Wild Ostrea edulis with Molecular Evidence of Infections with Bonamia spp. and/or Marteilia refringens Parasites
Open AccessArticle

Validation of a Laboratory-Developed Triplex Molecular Assay for Simultaneous Detection of Gastrointestinal Adenovirus and Rotavirus in Stool Specimens

1
Public Health Ontario Laboratory, 661 University Avenue, Toronto, ON M5G 1M1, Canada
2
Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 2V8, Canada
3
The Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(5), 326; https://doi.org/10.3390/pathogens9050326
Received: 14 March 2020 / Revised: 20 April 2020 / Accepted: 23 April 2020 / Published: 27 April 2020
Enteric viral pathogens causing gastroenteritis include adenovirus and rotavirus, among others. Rotavirus is the leading cause of severe diarrhea in infants and young children worldwide. Among the adenoviruses known to cause gastroenteritis are those of species F (serotypes 40, 41). Here, we describe the development and validation of a laboratory-developed gastrointestinal triplex rRT-PCR (triplex) assay that targets adenovirus and rotavirus. Stool specimens were tested from patients across Ontario. Specimens were previously tested for adenovirus and/or rotavirus by electron microscopy (EM) or immunochromatographic test (ICT). Triplex sensitivity, specificity, positive and negative predictive values compared to Seegene assay (a commercial assay used here as the standard reference method) were 100%, 97.8%, 86.0%, 100% for adenovirus, and 99.1%, 98.4%, 96.3%. 99.6% for rotavirus, respectively. The triplex assay had a 95.2% and 97.3% overall percent agreements (OPAs) when compared to EM for adenovirus or rotavirus detection, respectively, and an OPA of 90.9% when compared to rotavirus ICT for rotavirus detection. Triplex assay exhibited similar performance to the Seegene assay for both adenovirus and rotavirus and detected more adenovirus and rotavirus than traditional testing methods. The high performance along with lower cost and reduced turnaround time makes the triplex assay a desirable testing method for a clinical microbiology laboratory. View Full-Text
Keywords: multiplex; enteric; gastrointestinal; adenovirus; rotavirus; validation multiplex; enteric; gastrointestinal; adenovirus; rotavirus; validation
Show Figures

Figure 1

MDPI and ACS Style

Higgins, R.R.; Peci, A.; Cardona, M.; Gubbay, J.B. Validation of a Laboratory-Developed Triplex Molecular Assay for Simultaneous Detection of Gastrointestinal Adenovirus and Rotavirus in Stool Specimens. Pathogens 2020, 9, 326.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop