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Open AccessArticle

First Whole Genome Sequence of Anaplasma platys, an Obligate Intracellular Rickettsial Pathogen of Dogs

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Centro de Biología Celular y Molecular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT AIP), Ciudad del Saber, Panama City 0801, Panama
2
School of Veterinary Medicine, Ross University, Basseterre KN 0101, Saint Kitts and Nevis
*
Author to whom correspondence should be addressed.
Present Address: College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
Pathogens 2020, 9(4), 277; https://doi.org/10.3390/pathogens9040277
Received: 13 March 2020 / Revised: 2 April 2020 / Accepted: 8 April 2020 / Published: 10 April 2020
(This article belongs to the Section Animal Pathogens)
We have assembled the first genome draft of Anaplasma platys, an obligate intracellular rickettsia, and the only known bacterial pathogen infecting canine platelets. A. platys is a not-yet-cultivated bacterium that causes infectious cyclic canine thrombocytopenia, a potentially fatal disease in dogs. Despite its global distribution and veterinary relevance, no genome sequence has been published so far for this pathogen. Here, we used a strategy based on metagenome assembly to generate a draft of the A. platys genome using the blood of an infected dog. The assembled draft is similar to other Anaplasma genomes in size, gene content, and synteny. Notable differences are the apparent absence of rbfA, a gene encoding a 30S ribosome-binding factor acting as a cold-shock protein, as well as two genes involved in biotin metabolism. We also observed differences associated with expanded gene families, including those encoding outer membrane proteins, a type IV secretion system, ankyrin repeat-containing proteins, and proteins with predicted intrinsically disordered regions. Several of these families have members highly divergent in sequence, likely to be associated with survival and interactions within the host and the vector. The sequence of the A. platys genome can benefit future studies regarding invasion, survival, and pathogenesis of Anaplasma species, while paving the way for the better design of treatment and prevention strategies against these neglected intracellular pathogens. View Full-Text
Keywords: Anaplasma platys; dogs; whole genome sequence; tick-borne pathogens Anaplasma platys; dogs; whole genome sequence; tick-borne pathogens
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Llanes, A.; Rajeev, S. First Whole Genome Sequence of Anaplasma platys, an Obligate Intracellular Rickettsial Pathogen of Dogs. Pathogens 2020, 9, 277.

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