Next Article in Journal
Infection with Helicobacter pylori Induces Epithelial to Mesenchymal Transition in Human Cholangiocytes
Previous Article in Journal
Multilocus Genotyping Reveals New Molecular Markers for Differentiating Distinct Genetic Lineages among “Candidatus Phytoplasma Solani” Strains Associated with Grapevine Bois Noir
Open AccessArticle

A Quantitative Live-Cell Superresolution Imaging Framework for Measuring the Mobility of Single Molecules at Sites of Virus Assembly

Molecular and Cellular Biophysics Program, Department of Biological Sciences, University of Denver, Denver, CO 80210, USA
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(11), 972; https://doi.org/10.3390/pathogens9110972
Received: 23 September 2020 / Revised: 13 November 2020 / Accepted: 18 November 2020 / Published: 21 November 2020
The insurgence of superresolution microscopy into the fields of virology and microbiology has begun to enable the mapping of molecular assemblies critical for host–pathogen interfaces that organize on a scale below the resolution limit of the light microscope. It is, however, challenging to completely understand the molecular interactions between host and pathogen from strictly time-invariant observations. Herein, we describe a method using simultaneous dual-color superresolution microscopy to gain both structural and dynamic information about HIV-1 assembly. Specifically, we demonstrate the reconstruction of single virus assembly sites using live-cell photo-activated localization microscopy (PALM) while concurrently assessing the sub-viral mobility of the HIV-1 envelope glycoprotein during interaction with the viral lattice. We propose that our method is broadly applicable to elucidating pathogen and host protein–protein interactions through quantification of the dynamics of these proteins at the nanoscale. View Full-Text
Keywords: HIV-1; virus assembly; Env; Gag; single particle tracking; superresolution HIV-1; virus assembly; Env; Gag; single particle tracking; superresolution
Show Figures

Figure 1

MDPI and ACS Style

Groves, N.S.; Bruns, M.M.; van Engelenburg, S.B. A Quantitative Live-Cell Superresolution Imaging Framework for Measuring the Mobility of Single Molecules at Sites of Virus Assembly. Pathogens 2020, 9, 972.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop