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Article

Influence of Different Light Regimes on the Mycoparasitic Activity and 6-Pentyl-α-pyrone Biosynthesis in Two Strains of Trichoderma atroviride

1
Department of Microbiology, University of Innsbruck, 6020 Innsbruck, Austria
2
Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), 1180 Tulln, Austria
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(10), 860; https://doi.org/10.3390/pathogens9100860
Received: 8 September 2020 / Revised: 14 October 2020 / Accepted: 19 October 2020 / Published: 21 October 2020
(This article belongs to the Section Plant Pathogens)
The ascomycete Trichoderma atroviride is well known for its mycoparasitic lifestyle. Similar to other organisms, light is an important cue for T. atroviride. However, besides triggering of conidiation, little is known on the physiological responses of T. atroviride to light. In this study, we analyzed how cultivation under different light wavelengths and regimes impacted the behavior of two T. atroviride wild-type strains: IMI206040 and P1. While colony extension of both strains was slightly affected by light, massive differences in their photoconidation responses became evident. T. atroviride P1 colonies conidiated under all conditions tested including growth in complete darkness, while IMI206040 required white, blue or green light to trigger asexual reproduction. Interestingly, deletion of the stress-activated MAP kinase-encoding gene tmk3 abolished the ability of strain P1 to conidiate in red and yellow light as well as in darkness. Furthermore, light-dependent differences in the mycoparasitic activity and in the biosynthesis of the secondary metabolite 6-pentyl-α-pyrone (6-PP) became evident. 6-PP production was highest upon dark incubation, while light, especially exposure to white light as light/dark cycles, had an inhibitory effect on its biosynthesis. We conclude that the response of T. atroviride to light is strain-dependent and impacts differentiation, mycoparasitism, and 6-PP production; hence, this should be considered in experiments testing the mycoparasitic activity of these fungi. View Full-Text
Keywords: Trichoderma atroviride; mycoparasitism; secondary metabolites; 6-pentyl-α-pyrone; Tmk3 MAP kinase; light; dark Trichoderma atroviride; mycoparasitism; secondary metabolites; 6-pentyl-α-pyrone; Tmk3 MAP kinase; light; dark
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MDPI and ACS Style

Moreno-Ruiz, D.; Fuchs, A.; Missbach, K.; Schuhmacher, R.; Zeilinger, S. Influence of Different Light Regimes on the Mycoparasitic Activity and 6-Pentyl-α-pyrone Biosynthesis in Two Strains of Trichoderma atroviride. Pathogens 2020, 9, 860. https://doi.org/10.3390/pathogens9100860

AMA Style

Moreno-Ruiz D, Fuchs A, Missbach K, Schuhmacher R, Zeilinger S. Influence of Different Light Regimes on the Mycoparasitic Activity and 6-Pentyl-α-pyrone Biosynthesis in Two Strains of Trichoderma atroviride. Pathogens. 2020; 9(10):860. https://doi.org/10.3390/pathogens9100860

Chicago/Turabian Style

Moreno-Ruiz, Dubraska, Alessandro Fuchs, Kristina Missbach, Rainer Schuhmacher, and Susanne Zeilinger. 2020. "Influence of Different Light Regimes on the Mycoparasitic Activity and 6-Pentyl-α-pyrone Biosynthesis in Two Strains of Trichoderma atroviride" Pathogens 9, no. 10: 860. https://doi.org/10.3390/pathogens9100860

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