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Open AccessArticle

Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition

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Microbiology Program, Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
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Division of Neonatology, Departments of Pediatrics and Cell, Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
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Department of Biological Sciences, College of Science, Technology, Engineering and Mathematics, Alabama State University, Montgomery, AL 36104, USA
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Center for NanoBiotechnology Research (CNBR), Alabama State University, Montgomery, AL 36104, USA
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Department of Microbiology, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
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Division of Cardiovascular Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 297; https://doi.org/10.3390/pathogens8040297
Received: 1 November 2019 / Revised: 29 November 2019 / Accepted: 10 December 2019 / Published: 14 December 2019
The packaging of molecular constituents inside extracellular vesicles (EVs) allows them to participate in intercellular communication and the transfer of biological molecules, however the role of EVs during bacterial infection is poorly understood. The goal of this study was to examine the effects of Pseudomonas aeruginosa (P. aeruginosa) infection on the biogenesis and composition of EVs derived from the mouse microglia cell line, BV-2. BV-2 cells were cultured in exosome-free media and infected with 0, 1.3 × 104, or 2.6 × 104 colony forming units per milliliter P. aeruginosa for 72 h. The results indicated that compared with the control group, BV-2 cell viability significantly decreased after P. aeruginosa infection and BV-2-derived EVs concentration decreased significantly in the P. aeruginosa-infected group. P. aeruginosa infection significantly decreased chemokine ligand 4 messenger RNA in BV-2-derived infected EVs, compared with the control group (p ≤ 0.05). This study also revealed that heat shock protein 70 (p ≤ 0.05) and heat shock protein 90β (p ≤ 0.001) levels of expression within EVs increased after P. aeruginosa infection. EV treatment with EVs derived from P. aeruginosa infection reduced cell viability of BV-2 cells. P. aeruginosa infection alters the expression of specific proteins and mRNA in EVs. Our study suggests that P. aeruginosa infection modulates EV biogenesis and composition, which may influence bacterial pathogenesis and infection. View Full-Text
Keywords: Extracellular vesicles; exosome; biogenesis; microglia; Pseudomonas aeruginosa Extracellular vesicles; exosome; biogenesis; microglia; Pseudomonas aeruginosa
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MDPI and ACS Style

Jones, L.B.; Kumar, S.; Bell, C.R.; Peoples, V.A.; Crenshaw, B.J.; Coats, M.T.; Scoffield, J.A.; Rowe, G.C.; Sims, B.; Matthews, Q.L. Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition. Pathogens 2019, 8, 297.

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