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Molecular Detection and Characterization of Borrelia garinii (Spirochaetales: Borreliaceae) in Ixodes nipponensis (Ixodida: Ixodidae) Parasitizing a Dog in Korea

1
College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea
2
Department of Parasitology and Tropical Medicine, School of Medicine, Kyungpook National University, Daegu 41944, Korea
3
Department of Biology, School of Arts and Sciences, University of San Carlos, Talamban Campus, Cebu 6000, Philippines
4
College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea
5
Cardiovascular Research Institute, Kyungpook National University, Daegu 41566, Korea
*
Author to whom correspondence should be addressed.
Pathogens 2019, 8(4), 289; https://doi.org/10.3390/pathogens8040289
Received: 12 November 2019 / Revised: 1 December 2019 / Accepted: 4 December 2019 / Published: 6 December 2019
(This article belongs to the Section Animal Pathogens)
The present study aimed to detect and characterize Borrelia spp. in ticks attached to dogs in Korea. Overall, 562 ticks (276 pools) attached to dogs were collected and tested for Borrelia infection by PCR targeting the 5S-23S rRNA intergenic spacer region (rrf-rrl). One tick larva (pool level, 0.4%; individual level, 0.2%) was confirmed by sequencing Borrelia garinii, a zoonotic pathogen. For molecular characterization, the outer surface protein A (ospA) and flagellin genes were analyzed. Phylogenetic ospA analysis distinguished B. garinii from B. bavariensis, which has been recently identified as a novel Borrelia species. On the other hand, phylogenetic analysis showed that single gene analysis involving rrf-rrl or flagellin was not sufficient to differentiate B. garinii from B. bavariensis. In addition, the B. garinii-infected tick was identified as Ixodes nipponensis by sequencing according to mitochondrial 16S rRNA and the second transcribed spacer region. To our knowledge, this is the first study to report the molecular detection of B. garinii in I. nipponensis parasitizing a dog in Korea. Continuous monitoring of tick-borne pathogens in ticks attached to animals is required to avoid disease distribution and possible transmission to humans. View Full-Text
Keywords: Borrelia garinii; dog; Ixodes nipponensis; Lyme borreliosis; differential diagnosis; tick-borne pathogen Borrelia garinii; dog; Ixodes nipponensis; Lyme borreliosis; differential diagnosis; tick-borne pathogen
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Lee, S.-H.; Goo, Y.-K.; Geraldino, P.J.L.; Kwon, O.-D.; Kwak, D. Molecular Detection and Characterization of Borrelia garinii (Spirochaetales: Borreliaceae) in Ixodes nipponensis (Ixodida: Ixodidae) Parasitizing a Dog in Korea. Pathogens 2019, 8, 289.

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