A Dual Role for Macrophages in Modulating Lung Tissue Damage/Repair during L2 Toxocara canis Infection
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Description: Figure S1. Collagen deposition. Paraffin sections of 4µm thick from lung tissue, were Masson´s Trichrome stained and observed in microscopy light. Images were photographed with the 20x objective at 7, 28 and 60 dpi in WT, STAT1-/-, and STAT6-/- mice and collagen deposition was evaluated. Figure S2. : M1 and M2 markers after macrophages. WT, STAT1-/- and STAT6-/- mice were treated either with PBS-liposomes or clodronate-liposomes intratracheally to deplete macrophages, before and after being infected with 500 L2 T. canis larvae. The animals were euthanized at 4 dpi, lungs cells were collected, and flow cytometry was used to determine M1 or M2 activation. Representative dot plots and their respective percentage of F4/80+CD86+ (a), F4/80+IL-4Rα+ (b) and F4/80+MMR+ (c) double-positive cells are shown. Each dot plot represents an individual mouse. One-way ANOVA and Tukey's multiple comparison test. *P<0.05 comparing WT versus STAT1-/- and STAT6-/- mice. Figure S3. Numbers of cells in lung tissue. From inflammatory infiltrate in H&E stained sections the number of lymphocytes (a), macrophages (b) and PMN (c) was quantified in light microscopy (100x objective) images in WT (white bars), STAT1-/- (gray bars) and STAT6-/- (black bars) mice. Data are shown from two independent experiments as mean ± SEM (n = 6 per group) . Two-way ANOVA with Tukey multi-comparison test. *P<0.05 comparing WT versus STAT1-/- and STAT6-/-, and STAT1-/- versus STAT6-/- mice, at the same time point of infection.
Faz-López, B.; Mayoral-Reyes, H.; Hernández-Pando, R.; Martínez-Labat, P.; McKay, D.M.; Medina-Andrade, I.; Olguín, J.E.; Terrazas, L.I. A Dual Role for Macrophages in Modulating Lung Tissue Damage/Repair during L2 Toxocara canis Infection. Pathogens 2019, 8, 280. https://doi.org/10.3390/pathogens8040280
Faz-López B, Mayoral-Reyes H, Hernández-Pando R, Martínez-Labat P, McKay DM, Medina-Andrade I, Olguín JE, Terrazas LI. A Dual Role for Macrophages in Modulating Lung Tissue Damage/Repair during L2 Toxocara canis Infection. Pathogens. 2019; 8(4):280. https://doi.org/10.3390/pathogens8040280Chicago/Turabian Style
Faz-López, Berenice, Héctor Mayoral-Reyes, Rogelio Hernández-Pando, Pablo Martínez-Labat, Derek M. McKay, Itzel Medina-Andrade, Jonadab E. Olguín, and Luis I. Terrazas. 2019. "A Dual Role for Macrophages in Modulating Lung Tissue Damage/Repair during L2 Toxocara canis Infection" Pathogens 8, no. 4: 280. https://doi.org/10.3390/pathogens8040280