Genome Mining and Comparative Analysis of Streptococcus intermedius Causing Brain Abscess in a Child
AbstractStreptococcus intermedius (SI) is associated with prolonged hospitalization and low survival rates. The genetic mechanisms involved in brain abscess development and genome evolution in comparison to other members of the Streptococcus anginosus group are understudied. We performed a whole-genome comparative analysis of an SI isolate, LAU_SINT, associated with brain abscess following sinusitis with all SI genomes in addition to S. constellatus and S. anginosus. Selective pressure on virulence factors, phages, pan-genome evolution and single-nucleotide polymorphism analysis were assessed. The structural details of the type seven secretion system (T7SS) was elucidated and compared with different organisms. ily and nanA were both abundant and conserved. Nisin resistance determinants were found in 47% of the isolates. Pan-genome and SNPs-based analysis didn’t reveal significant geo-patterns. Our results showed that two SC isolates were misidentified as SI. We propose the presence of four T7SS modules (I–IV) located on various genomic islands. We detected a variety of factors linked to metal ions binding on the GIs carrying T7SS. This is the first detailed report characterizing the T7SS and its link to nisin resistance and metal ions binding in SI. These and yet uncharacterized T7SS transmembrane proteins merit further studies and could represent potential therapeutic targets. View Full-Text
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Issa, E.; Salloum, T.; Panossian, B.; Ayoub, D.; Abboud, E.; Tokajian, S. Genome Mining and Comparative Analysis of Streptococcus intermedius Causing Brain Abscess in a Child. Pathogens 2019, 8, 22.
Issa E, Salloum T, Panossian B, Ayoub D, Abboud E, Tokajian S. Genome Mining and Comparative Analysis of Streptococcus intermedius Causing Brain Abscess in a Child. Pathogens. 2019; 8(1):22.Chicago/Turabian Style
Issa, Elio; Salloum, Tamara; Panossian, Balig; Ayoub, David; Abboud, Edmond; Tokajian, Sima. 2019. "Genome Mining and Comparative Analysis of Streptococcus intermedius Causing Brain Abscess in a Child." Pathogens 8, no. 1: 22.
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