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Biochemical Characterization of Prion Strains in Bank Voles

Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena, Rome 299-00164, Italy
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Pathogens 2013, 2(3), 446-456; https://doi.org/10.3390/pathogens2030446
Received: 22 May 2013 / Revised: 25 June 2013 / Accepted: 26 June 2013 / Published: 2 July 2013
(This article belongs to the Special Issue Prions)
Prions exist as different strains exhibiting distinct disease phenotypes. Currently, the identification of prion strains is still based on biological strain typing in rodents. However, it has been shown that prion strains may be associated with distinct PrPSc biochemical types. Taking advantage of the availability of several prion strains adapted to a novel rodent model, the bank vole, we investigated if any prion strain was actually associated with distinctive PrPSc biochemical characteristics and if it was possible to univocally identify strains through PrPSc biochemical phenotypes. We selected six different vole-adapted strains (three human-derived and three animal-derived) and analyzed PrPSc from individual voles by epitope mapping of protease resistant core of PrPSc (PrPres) and by conformational stability and solubility assay. Overall, we discriminated five out of six prion strains, while two different scrapie strains showed identical PrPSc types. Our results suggest that the biochemical strain typing approach here proposed was highly discriminative, although by itself it did not allow us to identify all prion strains analyzed. View Full-Text
Keywords: prion; prion strain; TSE; PrPSc; bank vole; CJD; scrapie; BSE; conformational stability; GdnHCl prion; prion strain; TSE; PrPSc; bank vole; CJD; scrapie; BSE; conformational stability; GdnHCl
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Pirisinu, L.; Marcon, S.; Di Bari, M.A.; D'Agostino, C.; Agrimi, U.; Nonno, R. Biochemical Characterization of Prion Strains in Bank Voles. Pathogens 2013, 2, 446-456.

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