Next Article in Journal
Evaluation of Gastrointestinal Endoparasites in Cattle in Central Spain: Focus on Calicophoron daubneyi with Coprological, Epidemiological, and Anthelmintic Insights
Previous Article in Journal
Prevalence of Rotavirus in Diarrheic Piglets on RVA-Vaccinated and Non-Vaccinated Farms
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Pathogens
Volume 14
Issue 10
10.3390/pathogens14101056
pathogens-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Comparative Efficacy of Horse and Chicken Serum for the In Vitro Cultivation of Mycoplasma hyorhinis Clinical Isolates

by
Yi-Chia Li
1,2,3,
Yu-Wei Tseng
1,
Wei-Hao Lin
1,2,3,
Chao-Nan Lin
1,2,3 and
Ming-Tang Chiou
1,2,3,*
1
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912301, Taiwan
2
Animal Disease Diagnostic Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912301, Taiwan
3
Sustainable Swine Research Center, National Pingtung University of Science and Technology, Pingtung 912301, Taiwan
*
Author to whom correspondence should be addressed.
Pathogens 2025, 14(10), 1056; https://doi.org/10.3390/pathogens14101056
Submission received: 31 August 2025 / Revised: 1 October 2025 / Accepted: 17 October 2025 / Published: 19 October 2025
Browse Figures
Versions Notes

Abstract

Mycoplasma hyorhinis is an important respiratory pathogen in swine, yet optimal culture conditions for high-yield propagation remain undefined. This study compared horse serum (HS) and chicken serum (CS) at graded concentrations (10%, 20%, 30%) for their ability to support in vitro growth of four clinical M. hyorhinis isolates (strains A, B, C, and D). Cultures were prepared in modified Friis medium, and growth performance was assessed by final titer (color changing unit, CCU/mL) and time-to-detection at 102 and 104 CCU/mL. All media supported growth, but HS consistently outperformed CS in both yield and growth kinetics. The highest titers (109 CCU/mL) and shortest detection times (3.6–6 days) were observed in 20% HS for most strains. Increasing HS concentration to 30% reduced yield for several strains, suggesting a concentration-dependent inhibitory effect. CS demonstrated limited but strain-dependent growth support, with comparable performance to HS for strain B at lower thresholds. These findings identify 20% HS as an optimal supplement for efficient M. hyorhinis cultivation, while highlighting the potential of CS as a cost-effective alternative under certain conditions, with implications for diagnostic reagent production and vaccine development.
Keywords: Mycoplasma hyorhinis; horse serum; chicken serum; culture medium optimization; CCU (color changing unit) Mycoplasma hyorhinis; horse serum; chicken serum; culture medium optimization; CCU (color changing unit)

Share and Cite

MDPI and ACS Style

Li, Y.-C.; Tseng, Y.-W.; Lin, W.-H.; Lin, C.-N.; Chiou, M.-T. Comparative Efficacy of Horse and Chicken Serum for the In Vitro Cultivation of Mycoplasma hyorhinis Clinical Isolates. Pathogens 2025, 14, 1056. https://doi.org/10.3390/pathogens14101056

AMA Style

Li Y-C, Tseng Y-W, Lin W-H, Lin C-N, Chiou M-T. Comparative Efficacy of Horse and Chicken Serum for the In Vitro Cultivation of Mycoplasma hyorhinis Clinical Isolates. Pathogens. 2025; 14(10):1056. https://doi.org/10.3390/pathogens14101056

Chicago/Turabian Style

Li, Yi-Chia, Yu-Wei Tseng, Wei-Hao Lin, Chao-Nan Lin, and Ming-Tang Chiou. 2025. "Comparative Efficacy of Horse and Chicken Serum for the In Vitro Cultivation of Mycoplasma hyorhinis Clinical Isolates" Pathogens 14, no. 10: 1056. https://doi.org/10.3390/pathogens14101056

APA Style

Li, Y.-C., Tseng, Y.-W., Lin, W.-H., Lin, C.-N., & Chiou, M.-T. (2025). Comparative Efficacy of Horse and Chicken Serum for the In Vitro Cultivation of Mycoplasma hyorhinis Clinical Isolates. Pathogens, 14(10), 1056. https://doi.org/10.3390/pathogens14101056

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop