Brucellosis Seropositivity Using Three Serological Tests and Associated Risk Factors in Abattoir Workers in Gauteng Province, South Africa
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Study Design
2.3. Sampling Method and Recruitment
2.4. Study Population
2.5. Sample Size of Abattoir Workers
2.6. Pre-Testing of the Questionnaire and Data Collection
2.7. Data Collection
2.8. Collection of Blood Samples
2.9. Serological Tests Conducted
2.10. Statistical Analysis
2.11. Ethical Approval
3. Results
3.1. Description of Participants
3.2. Analysis of Seropositivity by Different Tests among the Abattoir Workers
3.3. Descriptive Statistics and Univariable Analysis of Factors among the Respondents Predictive of Brucella Seropositivity
4. Discussion
5. Conclusions
6. Limitations of this Study
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Test | No. of Sera Tested | No. (%) * Positive | 95% CI * |
---|---|---|---|
RBT | 103 | 13 (12.6) | 6.89–20.62 |
BrucellaCapt | 103 | 9 (8.7) | 4.07–15.94 |
IgG-ELISA | 103 | 18 (17.5) | 10.70–26.21 |
RBT/BrucellaCapt | 103 | 7 (6.8) | 2.78–13.50 |
RBT/IgG-ELISA | 103 | 10 (9.7) | 4.75–17.13 |
BrucellaCapt/IgG-ELISA | 103 | 9 (8.7) | 4.07–15.94 |
RBT/BrucellaCapt/IgG-ELISA | 103 | 7 (6.8) | 2.78–13.50 |
Variable | Category | Number of Humans Sampled | Number Positive | Percentage of Seropositive | Odds Ratio (95% CI *) | p-Value |
---|---|---|---|---|---|---|
Abattoir type | High-throughput Low-throughput | 55 48 | 7 14 | 12.7 29.2 | 2.79 (0.94, 9.11) | 0.039 |
Gender | Female Male | 16 87 | 1 20 | 6.3 23.0 | 4.44(0.61, 197.62) | 0.183 |
Age category | 18–30 years 31–60 years | 32 71 | 5 16 | 15.6 22.5 | 1.56 (0.48, 6.05) | 0.421 |
Marital status | Married Single | 42 61 | 8 13 | 19.0 21.3 | 1.15 (0.39, 3.57) | 0.779 |
Job description | Other Slaughterer/offal handling | 28 75 | 4 17 | 14.3 22.7 | 1.75 (0.49, 7.89) | 0.420 |
Days at work | 1 to 4 days 5 to 7 days | 4 99 | 2 19 | 0.5 19.2 | 0.24 (0.017, 3.53) | 0.184 |
Years at work | More than 1 year Up to 1 year | 82 21 | 16 5 | 19.5 23.8 | 1.29 (0.32, 4.44) | 0.663 |
Working on farms other than abattoirs? | No Yes | 65 38 | 16 5 | 24.6 13.2 | 0.47 (0.12, 1.51) | 0.164 |
Do you consume unpasteurized milk? | No Yes | 77 26 | 16 5 | 20.8 19.2 | 0.91 (0.23, 3.03) | 0.865 |
Do you slaughter animals at home? | No Yes | 54 49 | 8 13 | 14.8 26.5 | 2.06 (0.70, 6.41) | 0.141 |
Do you eat raw or undercooked meat? | No Yes | 91 12 | 20 1 | 22.0 8.3 | 0.33 (0.007, 2.49) | 0.451 |
Do you always wear your PPE? | No Yes | 6 97 | 0 21 | 0.0 21.6 | Infinity | 0.342 |
Do you know of brucellosis? | No Yes | 76 27 | 18 3 | 23.7 11.1 | 0.41 (0.07, 1.59) | 0.265 |
Do you think you can get brucellosis from animals? | No Yes | 81 22 | 10 0 | 12.3 0 | 0.33 (0.03, 1.57) | 0.231 |
Have you had cut/hand-cut injuries on duty? | No Yes | 26 77 | 6 15 | 23.1 19.5 | 0.81 (0.25, 2.89) | 0.694 |
Have you ever been diagnosed with brucellosis? | No Yes | 101 2 | 20 1 | 19.8 50 | 3.98 (0.05, 320.83) | 0.368 |
Have you ever had animal blood splashed on your face? | No Yes | 23 80 | 3 18 | 13.0 22.5 | 1.92 (0.48,11.25) | 0.393 |
Multivariable Analysis | |||
---|---|---|---|
Variable | Category | Odds Ratio (95% CI *) | p-Value |
Abattoir | High-throughput (ref) | ||
Low-throughput | 5.32 (0.64, 43.93) | 0.03 | |
Gender | Female (ref) | ||
Male | 3.11 (1.12, 8.67) | 0.12 |
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Kolo, F.B.; Adesiyun, A.A.; Fasina, F.O.; Harris, B.N.; Rossouw, J.; Byaruhanga, C.; Geyer, H.D.W.; Blumberg, L.; Frean, J.; van Heerden, H. Brucellosis Seropositivity Using Three Serological Tests and Associated Risk Factors in Abattoir Workers in Gauteng Province, South Africa. Pathogens 2024, 13, 64. https://doi.org/10.3390/pathogens13010064
Kolo FB, Adesiyun AA, Fasina FO, Harris BN, Rossouw J, Byaruhanga C, Geyer HDW, Blumberg L, Frean J, van Heerden H. Brucellosis Seropositivity Using Three Serological Tests and Associated Risk Factors in Abattoir Workers in Gauteng Province, South Africa. Pathogens. 2024; 13(1):64. https://doi.org/10.3390/pathogens13010064
Chicago/Turabian StyleKolo, Francis B., Abiodun A. Adesiyun, Folorunso O. Fasina, Bernice N. Harris, Jennifer Rossouw, Charles Byaruhanga, Hermanus De Wet Geyer, Lucille Blumberg, John Frean, and Henriette van Heerden. 2024. "Brucellosis Seropositivity Using Three Serological Tests and Associated Risk Factors in Abattoir Workers in Gauteng Province, South Africa" Pathogens 13, no. 1: 64. https://doi.org/10.3390/pathogens13010064
APA StyleKolo, F. B., Adesiyun, A. A., Fasina, F. O., Harris, B. N., Rossouw, J., Byaruhanga, C., Geyer, H. D. W., Blumberg, L., Frean, J., & van Heerden, H. (2024). Brucellosis Seropositivity Using Three Serological Tests and Associated Risk Factors in Abattoir Workers in Gauteng Province, South Africa. Pathogens, 13(1), 64. https://doi.org/10.3390/pathogens13010064