Monocytes from Uninfected Neonates Born to Trypanosoma cruzi-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Blood Obtention
2.2. Purification of Cord Blood Mononuclear Cells and Monocytes
2.3. T. cruzi Infection of Monocytes
2.4. TNF-α Production by Monocytes
2.5. Phenotypic Characterization of Monocytes by Flow Cytometry
2.6. Depletion of T. cruzi-Specific Antibodies by Affinity Chromatography
3. Results
3.1. Monocytes from M+B- Newborns Are Primed to Produce Higher TNF-α Levels
3.2. Susceptibility of M+B- Monocytes to T. cruzi Infection
3.3. Autologous Cord Plasma Limits Intracellular T. cruzi Multiplication
3.4. M+B- Monocytes Are Occasionally Able to Completely Control Intracellular Infection
3.5. T. cruzi–Specific Antibodies Present in the Plasma of M+B- Newborns Improve the Control of the Infection
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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M-B- | M+B- | ||
---|---|---|---|
CD54 | % (a) | 67.1 ± 6.2 (10) | 56.1 ± 8.0 (16) |
MFI (b) | 14.1 ± 1.1 | 12.2 ± 2.2 | |
HLA-DR | % (a) | 98.0 ± 0.3 (10) | 90.6 ± 4.9 (16) |
MFI (b) | 95.9 ± 6.8 | 86.2 ± 12.4 | |
TLR2 | % (a) | 98.1 ± 0.5 (9) | 96.2 ± 1.5 (11) |
MFI (b) | 58.8 ± 0.5 | 52.3 ± 4.4 | |
TLR4 | % (a) | 76.0 ± 4.8 (9) | 75.6 ± 5.1 (11) |
MFI (b) | 30.8 ± 1.5 | 28.6 ± 2.0 |
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Flores, A.; Alonso-Vega, C.; Hermann, E.; Torrico, M.-C.; Montaño Villarroel, N.A.; Torrico, F.; Carlier, Y.; Truyens, C. Monocytes from Uninfected Neonates Born to Trypanosoma cruzi-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies. Pathogens 2023, 12, 1103. https://doi.org/10.3390/pathogens12091103
Flores A, Alonso-Vega C, Hermann E, Torrico M-C, Montaño Villarroel NA, Torrico F, Carlier Y, Truyens C. Monocytes from Uninfected Neonates Born to Trypanosoma cruzi-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies. Pathogens. 2023; 12(9):1103. https://doi.org/10.3390/pathogens12091103
Chicago/Turabian StyleFlores, Amilcar, Cristina Alonso-Vega, Emmanuel Hermann, Mary-Cruz Torrico, Nair Alaide Montaño Villarroel, Faustino Torrico, Yves Carlier, and Carine Truyens. 2023. "Monocytes from Uninfected Neonates Born to Trypanosoma cruzi-Infected Mothers Display Upregulated Capacity to Produce TNF-α and to Control Infection in Association with Maternally Transferred Antibodies" Pathogens 12, no. 9: 1103. https://doi.org/10.3390/pathogens12091103