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Volume 11
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Article

Genome-Wide Association Study for Haemonchus contortus Resistance in Morada Nova Sheep

by
Simone Cristina Méo Niciura
1,*,
Magda Vieira Benavides
2,
Cintia Hiromi Okino
1,
Adriana Mercia Guaratini Ibelli
3,
Alessandro Pelegrine Minho
1,
Sergio Novita Esteves
1 and
Ana Carolina de Souza Chagas
1
1
Embrapa Pecuária Sudeste, São Carlos 13560-970, SP, Brazil
2
Embrapa Pecuária Sul, Bagé 96401-970, RS, Brazil
3
Embrapa Suínos e Aves, Concórdia 89715-899, SC, Brazil
*
Author to whom correspondence should be addressed.
Pathogens 2022, 11(8), 939; https://doi.org/10.3390/pathogens11080939
Submission received: 14 July 2022 / Revised: 15 August 2022 / Accepted: 17 August 2022 / Published: 19 August 2022
(This article belongs to the Special Issue Recent Advances in the Control of Ecto and Endo-Parasites in Ruminant from a Sustainable Perspective)
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Abstract

Among the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case–control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, OAR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification.
Keywords: gastrointestinal nematode control; genotyping; GWAS; molecular markers; ovine; parasite resistance gastrointestinal nematode control; genotyping; GWAS; molecular markers; ovine; parasite resistance

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MDPI and ACS Style

Niciura, S.C.M.; Benavides, M.V.; Okino, C.H.; Ibelli, A.M.G.; Minho, A.P.; Esteves, S.N.; Chagas, A.C.d.S. Genome-Wide Association Study for Haemonchus contortus Resistance in Morada Nova Sheep. Pathogens 2022, 11, 939. https://doi.org/10.3390/pathogens11080939

AMA Style

Niciura SCM, Benavides MV, Okino CH, Ibelli AMG, Minho AP, Esteves SN, Chagas ACdS. Genome-Wide Association Study for Haemonchus contortus Resistance in Morada Nova Sheep. Pathogens. 2022; 11(8):939. https://doi.org/10.3390/pathogens11080939

Chicago/Turabian Style

Niciura, Simone Cristina Méo, Magda Vieira Benavides, Cintia Hiromi Okino, Adriana Mercia Guaratini Ibelli, Alessandro Pelegrine Minho, Sergio Novita Esteves, and Ana Carolina de Souza Chagas. 2022. "Genome-Wide Association Study for Haemonchus contortus Resistance in Morada Nova Sheep" Pathogens 11, no. 8: 939. https://doi.org/10.3390/pathogens11080939

APA Style

Niciura, S. C. M., Benavides, M. V., Okino, C. H., Ibelli, A. M. G., Minho, A. P., Esteves, S. N., & Chagas, A. C. d. S. (2022). Genome-Wide Association Study for Haemonchus contortus Resistance in Morada Nova Sheep. Pathogens, 11(8), 939. https://doi.org/10.3390/pathogens11080939

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