A Modular Hepatitis E Virus Replicon System for Studies on the Role of ORF1-Encoded Polyprotein Domains
Abstract
:1. Introduction
2. Results
2.1. Construction of Luciferase Reporter Replicons Based on Different HEV-3 Strains
2.2. Rabbit HEV-Based Replicons Generate Low Luciferase Activity
2.3. Construction and Evaluation of Chimeric ORF1 HEV Replicons
3. Discussion
4. Materials and Methods
4.1. HEV Strains, Plasmids, and Cell Culture
4.2. PCR and Cloning
4.3. In Vitro Transcription and Transfection
4.4. Luciferase Reporter Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Replicon Nucleic Acid Sequence Identity | ORF1 Nucleic Acid Sequence Identity | ORF1 Amino Acid Sequence Identity | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
rab81 | rab52 | 47832mc | rab81 | rab52 | 47832mc | rab81 | rab52 | 47832mc | |||
rab52 | 77.7% | rab52 | 74.4% | rab52 | 86.7% | ||||||
47832mc | 78.4% | 77.5% | 47832mc | 75.1% | 73.5% | 47832mc | 86.5% | 85.3% | |||
p6 | 78.5% | 77.1% | 83.5% | p6 | 75.5% | 73.4% | 81.3% | p6 | 87% | 84.9% | 92.2% |
Nucleotide Sequence Identity | |||||||||||
MYP | VXH | RJ | |||||||||
rab81 | rab52 | 47832mc | rab81 | rab52 | 47832mc | rab81 | rab52 | 47832mc | |||
rab52 | 76.9% | rab52 | 68.6% | rab52 | 79% | ||||||
47832mc | 79.1% | 78.4% | 47832mc | 67.7% | 63.7% | 47832mc | 79.9% | 81.4% | |||
p6 | 80.4% | 78.5% | 84.9% | p6 | 67.7% | 63.7% | 75.3% | p6 | 79.4% | 80.6% | 84.7% |
Amino Acid Sequence Identity | |||||||||||
MYP | VXH | RJ | |||||||||
rab81 | rab52 | 47832mc | rab81 | rab52 | 47832mc | rab81 | rab52 | 47832mc | |||
rab52 | 88.5% | rab52 | 81% | rab52 | 91.9% | ||||||
47832mc | 90.9% | 91.1% | 47832mc | 77.7% | 72.8% | 47832mc | 92.4% | 95.5% | |||
p6 | 91.2% | 90.2% | 97.4% | p6 | 79.2% | 72.9% | 82% | p6 | 91.9% | 95% | 99% |
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Cierniak, F.; Ulrich, R.G.; Groschup, M.H.; Eiden, M. A Modular Hepatitis E Virus Replicon System for Studies on the Role of ORF1-Encoded Polyprotein Domains. Pathogens 2022, 11, 355. https://doi.org/10.3390/pathogens11030355
Cierniak F, Ulrich RG, Groschup MH, Eiden M. A Modular Hepatitis E Virus Replicon System for Studies on the Role of ORF1-Encoded Polyprotein Domains. Pathogens. 2022; 11(3):355. https://doi.org/10.3390/pathogens11030355
Chicago/Turabian StyleCierniak, Filip, Rainer G. Ulrich, Martin H. Groschup, and Martin Eiden. 2022. "A Modular Hepatitis E Virus Replicon System for Studies on the Role of ORF1-Encoded Polyprotein Domains" Pathogens 11, no. 3: 355. https://doi.org/10.3390/pathogens11030355