Prevalence of Pathogenic Leptospira spp. in Non-Volant Small Mammals of Hutan Lipur Sekayu, Terengganu, Malaysia
Abstract
:1. Introduction
2. Methods
2.1. Study Area
2.2. Animal Trapping and Sampling Collection
2.3. Ethical Statement
2.4. DNA Extraction
2.5. PCR Detection of Pathogenic Leptospira spp. and Sequencing
2.6. Data Analysis
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Order and Family | Scientific Name | Common Name | Total Individuals Captured | Total Positive Individuals (%) |
---|---|---|---|---|
Scandentia Tupaiidae | Tupaia glis | Common Treeshrew | 10 | 2 (20) |
Rodentia Muridae | Rattus tiomanicus | Malaysian Wood Rat | 22 | 4 (18.2) |
Rattus rattus | Black Rat | 1 | 0 (0) | |
Maxomys rajah | Rajah Spiny Rat | 4 | 1 (25) | |
Maxomys whiteheadi | Whitehead’s Spiny Rat | 1 | 1 (100) | |
Sundamys muelleri | Muller’s Giant Sunda Rat | 2 | 1 (50) | |
Rodentia Sciuridae | Collasciurus notatus | Plaintain Squirrel | 4 | 0 (0) |
Sundasciurus tenuis | Slender Squirrel | 1 | 0 (0) | |
Total | 45 | 9 (20) |
Lane | Species | Percent ID | Query Cover | Accession No. |
---|---|---|---|---|
1 (Batch 1) (R. tiomanicus) | Leptospira interrogans | 98.9% | 99% | CP044513.1 |
1 (Batch 2) M. whiteheadi | Leptospira interrogans | 97.6% | 99% | KY356922.1 |
2 (R. tiomanicus) | Leptospira interrogans | 98% | 99% | KY356922.1 |
3 (S. muelleri) | Uncultured Leptospira sp. | 97.5 | 98% | MG831575.1 |
5 (R. tiomanicus) | Uncultured Leptospira sp. | 98.3% | 97% | MG831575.1 |
6 (R. tiomanicus) | Leptospira interrogans | 95.9% | 99% | KY356922.1 |
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Shafie, N.J.; Halim, N.S.A.; Awoniyi, A.M.; Zalipah, M.N.; Md-Nor, S.; Nazri, M.U.I.A.; Costa, F. Prevalence of Pathogenic Leptospira spp. in Non-Volant Small Mammals of Hutan Lipur Sekayu, Terengganu, Malaysia. Pathogens 2022, 11, 1300. https://doi.org/10.3390/pathogens11111300
Shafie NJ, Halim NSA, Awoniyi AM, Zalipah MN, Md-Nor S, Nazri MUIA, Costa F. Prevalence of Pathogenic Leptospira spp. in Non-Volant Small Mammals of Hutan Lipur Sekayu, Terengganu, Malaysia. Pathogens. 2022; 11(11):1300. https://doi.org/10.3390/pathogens11111300
Chicago/Turabian StyleShafie, Nur Juliani, Najma Syahmin Abdul Halim, Adedayo Michael Awoniyi, Mohamed Nor Zalipah, Shukor Md-Nor, Mohd Ulul Ilmie Ahmad Nazri, and Federico Costa. 2022. "Prevalence of Pathogenic Leptospira spp. in Non-Volant Small Mammals of Hutan Lipur Sekayu, Terengganu, Malaysia" Pathogens 11, no. 11: 1300. https://doi.org/10.3390/pathogens11111300